Autologous fibrin gel

Bactericidal properties in contaminated hepatic injury

Scott A. Dulchavsky, Evan R. Geller, James Maurer, P. R. Kennedy, George T. Tortora, Subir R. Maitra

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Fibrin glue is an effective hemostatic agent in a variety of clinical situations; its utility is limited by potential transmission of viral infection. We studied the bactericidal properties of fibrin gel (FG) in a murine contaminated hepatic injury model and in vitro by agar plate culture method. Intra-abdominal abscess formation and adhesion rate were assessed following controlled liver injury in association with abdominal contamination with 107 Bacteroides fragilis and hepatorrhaphy (H, n = 15) or FG (n = 12). Animals treated by hepatorrhaphy had a significantly greater intra-abdominal abscess rate (15/15 vs. 4/12, p <0.05) and adhesion rate (14/15 vs. 6/12, p <0.05) than animals treated with FG. Fibrin gel is bactericidal to Bacteroides fragilis, Enterobacter faecium, Escherichia coli, and Staphylococcus aureus but has no effect against Klebsiella pneumoniae or Pseudomonas aeruginosa; the plasma component appears active. Fibrin gel demonstrates significant improvement in adhesion formation and intra-abdominal abscess rate when compared with suture hepatorrhaphy. Fibrin gel appears protective in contaminated hepatic injury.

Original languageEnglish (US)
Pages (from-to)991-995
Number of pages5
JournalJournal of Trauma - Injury, Infection and Critical Care
Volume31
Issue number7
StatePublished - 1991
Externally publishedYes

Fingerprint

Fibrin
Gels
Abdominal Abscess
Liver
Wounds and Injuries
Bacteroides fragilis
Enterobacter
Fibrin Tissue Adhesive
Klebsiella pneumoniae
Hemostatics
Virus Diseases
Pseudomonas aeruginosa
Sutures
Agar
Staphylococcus aureus
Escherichia coli

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Surgery

Cite this

Dulchavsky, S. A., Geller, E. R., Maurer, J., Kennedy, P. R., Tortora, G. T., & Maitra, S. R. (1991). Autologous fibrin gel: Bactericidal properties in contaminated hepatic injury. Journal of Trauma - Injury, Infection and Critical Care, 31(7), 991-995.

Autologous fibrin gel : Bactericidal properties in contaminated hepatic injury. / Dulchavsky, Scott A.; Geller, Evan R.; Maurer, James; Kennedy, P. R.; Tortora, George T.; Maitra, Subir R.

In: Journal of Trauma - Injury, Infection and Critical Care, Vol. 31, No. 7, 1991, p. 991-995.

Research output: Contribution to journalArticle

Dulchavsky, SA, Geller, ER, Maurer, J, Kennedy, PR, Tortora, GT & Maitra, SR 1991, 'Autologous fibrin gel: Bactericidal properties in contaminated hepatic injury', Journal of Trauma - Injury, Infection and Critical Care, vol. 31, no. 7, pp. 991-995.
Dulchavsky SA, Geller ER, Maurer J, Kennedy PR, Tortora GT, Maitra SR. Autologous fibrin gel: Bactericidal properties in contaminated hepatic injury. Journal of Trauma - Injury, Infection and Critical Care. 1991;31(7):991-995.
Dulchavsky, Scott A. ; Geller, Evan R. ; Maurer, James ; Kennedy, P. R. ; Tortora, George T. ; Maitra, Subir R. / Autologous fibrin gel : Bactericidal properties in contaminated hepatic injury. In: Journal of Trauma - Injury, Infection and Critical Care. 1991 ; Vol. 31, No. 7. pp. 991-995.
@article{d22e729db3754bbfbb4eda2afcf3e2a1,
title = "Autologous fibrin gel: Bactericidal properties in contaminated hepatic injury",
abstract = "Fibrin glue is an effective hemostatic agent in a variety of clinical situations; its utility is limited by potential transmission of viral infection. We studied the bactericidal properties of fibrin gel (FG) in a murine contaminated hepatic injury model and in vitro by agar plate culture method. Intra-abdominal abscess formation and adhesion rate were assessed following controlled liver injury in association with abdominal contamination with 107 Bacteroides fragilis and hepatorrhaphy (H, n = 15) or FG (n = 12). Animals treated by hepatorrhaphy had a significantly greater intra-abdominal abscess rate (15/15 vs. 4/12, p <0.05) and adhesion rate (14/15 vs. 6/12, p <0.05) than animals treated with FG. Fibrin gel is bactericidal to Bacteroides fragilis, Enterobacter faecium, Escherichia coli, and Staphylococcus aureus but has no effect against Klebsiella pneumoniae or Pseudomonas aeruginosa; the plasma component appears active. Fibrin gel demonstrates significant improvement in adhesion formation and intra-abdominal abscess rate when compared with suture hepatorrhaphy. Fibrin gel appears protective in contaminated hepatic injury.",
author = "Dulchavsky, {Scott A.} and Geller, {Evan R.} and James Maurer and Kennedy, {P. R.} and Tortora, {George T.} and Maitra, {Subir R.}",
year = "1991",
language = "English (US)",
volume = "31",
pages = "991--995",
journal = "Journal of Trauma and Acute Care Surgery",
issn = "2163-0755",
publisher = "Lippincott Williams and Wilkins",
number = "7",

}

TY - JOUR

T1 - Autologous fibrin gel

T2 - Bactericidal properties in contaminated hepatic injury

AU - Dulchavsky, Scott A.

AU - Geller, Evan R.

AU - Maurer, James

AU - Kennedy, P. R.

AU - Tortora, George T.

AU - Maitra, Subir R.

PY - 1991

Y1 - 1991

N2 - Fibrin glue is an effective hemostatic agent in a variety of clinical situations; its utility is limited by potential transmission of viral infection. We studied the bactericidal properties of fibrin gel (FG) in a murine contaminated hepatic injury model and in vitro by agar plate culture method. Intra-abdominal abscess formation and adhesion rate were assessed following controlled liver injury in association with abdominal contamination with 107 Bacteroides fragilis and hepatorrhaphy (H, n = 15) or FG (n = 12). Animals treated by hepatorrhaphy had a significantly greater intra-abdominal abscess rate (15/15 vs. 4/12, p <0.05) and adhesion rate (14/15 vs. 6/12, p <0.05) than animals treated with FG. Fibrin gel is bactericidal to Bacteroides fragilis, Enterobacter faecium, Escherichia coli, and Staphylococcus aureus but has no effect against Klebsiella pneumoniae or Pseudomonas aeruginosa; the plasma component appears active. Fibrin gel demonstrates significant improvement in adhesion formation and intra-abdominal abscess rate when compared with suture hepatorrhaphy. Fibrin gel appears protective in contaminated hepatic injury.

AB - Fibrin glue is an effective hemostatic agent in a variety of clinical situations; its utility is limited by potential transmission of viral infection. We studied the bactericidal properties of fibrin gel (FG) in a murine contaminated hepatic injury model and in vitro by agar plate culture method. Intra-abdominal abscess formation and adhesion rate were assessed following controlled liver injury in association with abdominal contamination with 107 Bacteroides fragilis and hepatorrhaphy (H, n = 15) or FG (n = 12). Animals treated by hepatorrhaphy had a significantly greater intra-abdominal abscess rate (15/15 vs. 4/12, p <0.05) and adhesion rate (14/15 vs. 6/12, p <0.05) than animals treated with FG. Fibrin gel is bactericidal to Bacteroides fragilis, Enterobacter faecium, Escherichia coli, and Staphylococcus aureus but has no effect against Klebsiella pneumoniae or Pseudomonas aeruginosa; the plasma component appears active. Fibrin gel demonstrates significant improvement in adhesion formation and intra-abdominal abscess rate when compared with suture hepatorrhaphy. Fibrin gel appears protective in contaminated hepatic injury.

UR - http://www.scopus.com/inward/record.url?scp=0026339479&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026339479&partnerID=8YFLogxK

M3 - Article

VL - 31

SP - 991

EP - 995

JO - Journal of Trauma and Acute Care Surgery

JF - Journal of Trauma and Acute Care Surgery

SN - 2163-0755

IS - 7

ER -