Autolysis of Escherichia coli and Bacillus subtilis cells in low gravity

M. A. Kacena, E. E. Smith, P. Todd

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The role of gravity in the autolysis of Bacillus subtilis and Escherichia coli was studied by growing cells on Earth and in microgravity on Space Station Mir. Autolysis analysis was completed by examining the death phase or exponential decay of cells for approximately 4 months following the stationary phase. Consistent with published findings, the stationary-phase cell population was 170% and 90% higher in flight B. subtilis and E. coli cultures, respectively, than in ground cultures. Although both flight autolysis curves began at higher cell densities than control curves, the rate of autolysis in flight cultures was identical to that of their respective ground control rates.

Original languageEnglish (US)
Pages (from-to)437-439
Number of pages3
JournalApplied Microbiology and Biotechnology
Volume52
Issue number3
DOIs
StatePublished - Sep 1 1999

Fingerprint

Hypogravity
Autolysis
Bacilli
Bacillus subtilis
Escherichia coli
Gravitation
Microgravity
Space stations
Earth (planet)
Cells
Weightlessness
Cell Count
Population

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Autolysis of Escherichia coli and Bacillus subtilis cells in low gravity. / Kacena, M. A.; Smith, E. E.; Todd, P.

In: Applied Microbiology and Biotechnology, Vol. 52, No. 3, 01.09.1999, p. 437-439.

Research output: Contribution to journalArticle

@article{1118edf3035c4e11ad05a9fb64197bdf,
title = "Autolysis of Escherichia coli and Bacillus subtilis cells in low gravity",
abstract = "The role of gravity in the autolysis of Bacillus subtilis and Escherichia coli was studied by growing cells on Earth and in microgravity on Space Station Mir. Autolysis analysis was completed by examining the death phase or exponential decay of cells for approximately 4 months following the stationary phase. Consistent with published findings, the stationary-phase cell population was 170{\%} and 90{\%} higher in flight B. subtilis and E. coli cultures, respectively, than in ground cultures. Although both flight autolysis curves began at higher cell densities than control curves, the rate of autolysis in flight cultures was identical to that of their respective ground control rates.",
author = "Kacena, {M. A.} and Smith, {E. E.} and P. Todd",
year = "1999",
month = "9",
day = "1",
doi = "10.1007/s002530051543",
language = "English (US)",
volume = "52",
pages = "437--439",
journal = "Applied Microbiology and Biotechnology",
issn = "0175-7598",
publisher = "Springer Verlag",
number = "3",

}

TY - JOUR

T1 - Autolysis of Escherichia coli and Bacillus subtilis cells in low gravity

AU - Kacena, M. A.

AU - Smith, E. E.

AU - Todd, P.

PY - 1999/9/1

Y1 - 1999/9/1

N2 - The role of gravity in the autolysis of Bacillus subtilis and Escherichia coli was studied by growing cells on Earth and in microgravity on Space Station Mir. Autolysis analysis was completed by examining the death phase or exponential decay of cells for approximately 4 months following the stationary phase. Consistent with published findings, the stationary-phase cell population was 170% and 90% higher in flight B. subtilis and E. coli cultures, respectively, than in ground cultures. Although both flight autolysis curves began at higher cell densities than control curves, the rate of autolysis in flight cultures was identical to that of their respective ground control rates.

AB - The role of gravity in the autolysis of Bacillus subtilis and Escherichia coli was studied by growing cells on Earth and in microgravity on Space Station Mir. Autolysis analysis was completed by examining the death phase or exponential decay of cells for approximately 4 months following the stationary phase. Consistent with published findings, the stationary-phase cell population was 170% and 90% higher in flight B. subtilis and E. coli cultures, respectively, than in ground cultures. Although both flight autolysis curves began at higher cell densities than control curves, the rate of autolysis in flight cultures was identical to that of their respective ground control rates.

UR - http://www.scopus.com/inward/record.url?scp=0032854415&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032854415&partnerID=8YFLogxK

U2 - 10.1007/s002530051543

DO - 10.1007/s002530051543

M3 - Article

C2 - 10531656

AN - SCOPUS:0032854415

VL - 52

SP - 437

EP - 439

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 0175-7598

IS - 3

ER -