Automated analysis of viral integration sites in gene therapy research using the SeqMap web resource

B. Peters, S. Dirscherl, J. Dantzer, J. Nowacki, S. Cross, X. Li, Kenneth Cornetta, M. C. Dinauer, S. D. Mooney

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Research in gene therapy involving genome-integrating vectors now often includes analysis of vector integration sites across the genome using methods such as ligation-mediated PCR (LM-PCR) or linear amplification-mediated PCR (LAM-PCR). To help researchers analyze these sites and the functions of nearby genes, we have developed SeqMap (http://seqmap.compbio.iupui.edu/) a secure, web-based comprehensive vector integration site management tool that automatically analyzes and annotates large numbers of vector integration sites derived from LM-PCR experiments in human and model organisms upon a common genome database. We believe the use of this resource will enable better reproducibility and understanding of this important data.

Original languageEnglish
Pages (from-to)1294-1298
Number of pages5
JournalGene Therapy
Volume15
Issue number18
DOIs
StatePublished - 2008

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Virus Integration
Genetic Therapy
Genome
Polymerase Chain Reaction
Ligation
Research
Research Personnel
Databases
Genes

ASJC Scopus subject areas

  • Genetics

Cite this

Peters, B., Dirscherl, S., Dantzer, J., Nowacki, J., Cross, S., Li, X., ... Mooney, S. D. (2008). Automated analysis of viral integration sites in gene therapy research using the SeqMap web resource. Gene Therapy, 15(18), 1294-1298. https://doi.org/10.1038/gt.2008.99

Automated analysis of viral integration sites in gene therapy research using the SeqMap web resource. / Peters, B.; Dirscherl, S.; Dantzer, J.; Nowacki, J.; Cross, S.; Li, X.; Cornetta, Kenneth; Dinauer, M. C.; Mooney, S. D.

In: Gene Therapy, Vol. 15, No. 18, 2008, p. 1294-1298.

Research output: Contribution to journalArticle

Peters, B, Dirscherl, S, Dantzer, J, Nowacki, J, Cross, S, Li, X, Cornetta, K, Dinauer, MC & Mooney, SD 2008, 'Automated analysis of viral integration sites in gene therapy research using the SeqMap web resource', Gene Therapy, vol. 15, no. 18, pp. 1294-1298. https://doi.org/10.1038/gt.2008.99
Peters, B. ; Dirscherl, S. ; Dantzer, J. ; Nowacki, J. ; Cross, S. ; Li, X. ; Cornetta, Kenneth ; Dinauer, M. C. ; Mooney, S. D. / Automated analysis of viral integration sites in gene therapy research using the SeqMap web resource. In: Gene Therapy. 2008 ; Vol. 15, No. 18. pp. 1294-1298.
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