Autonomous and nonautonomous regulation of Wnt-mediated neuronal polarity by the C. elegans Ror kinase CAM-1

Shih Chieh Jason Chien, Mark Gurling, Changsung Kim, Teresa Craft, Wayne Forrester, Gian Garriga

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Wnts are a conserved family of secreted glycoproteins that regulate various developmental processes in metazoans. Three of the five Caenorhabditis elegans Wnts, CWN-1, CWN-2 and EGL-20, and the sole Wnt receptor of the Ror kinase family, CAM-1, are known to regulate the anterior polarization of the mechanosensory neuron ALM. Here we show that CAM-1 and the Frizzled receptor MOM-5 act in parallel pathways to control ALM polarity. We also show that CAM-1 has two functions in this process: an autonomous signaling function that promotes anterior polarization and a nonautonomous Wnt-antagonistic function that inhibits anterior polarization. These antagonistic activities can account for the weak ALM phenotypes displayed by cam-1 mutants. Our observations suggest that CAM-1 could function as a Wnt receptor in many developmental processes, but the analysis of cam-1 mutants may fail to reveal CAM-1's role as a receptor in these processes because of its Wnt-antagonistic activity. In this model, loss of CAM-1 results in increased levels of Wnts that act through other Wnt receptors, masking CAM-1's autonomous role as a Wnt receptor.

Original languageEnglish (US)
Pages (from-to)55-65
Number of pages11
JournalDevelopmental Biology
Volume404
Issue number1
DOIs
StatePublished - Aug 1 2015

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Wnt Receptors
Phosphotransferases
Frizzled Receptors
Caenorhabditis elegans
Glycoproteins
Phenotype
Neurons

Keywords

  • C. elegans
  • CAM-1
  • Neuronal polarity
  • Ror kinase

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Molecular Biology

Cite this

Autonomous and nonautonomous regulation of Wnt-mediated neuronal polarity by the C. elegans Ror kinase CAM-1. / Chien, Shih Chieh Jason; Gurling, Mark; Kim, Changsung; Craft, Teresa; Forrester, Wayne; Garriga, Gian.

In: Developmental Biology, Vol. 404, No. 1, 01.08.2015, p. 55-65.

Research output: Contribution to journalArticle

Chien, Shih Chieh Jason ; Gurling, Mark ; Kim, Changsung ; Craft, Teresa ; Forrester, Wayne ; Garriga, Gian. / Autonomous and nonautonomous regulation of Wnt-mediated neuronal polarity by the C. elegans Ror kinase CAM-1. In: Developmental Biology. 2015 ; Vol. 404, No. 1. pp. 55-65.
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