Autonomous murine T-cell progenitor production in the extra-embryonic yolk sac before HSC emergence

Momoko Yoshimoto, Prashanth Porayette, Nicole L. Glosson, Simon J. Conway, Nadia Carlesso, Angelo A. Cardoso, Mark H. Kaplan, Mervin C. Yoder

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

The extra-embryonic yolk sac (YS) is the first hematopoietic site in the mouse embryo and is thought to generate only primitive erythroid and myeloerythroid progenitor cells before definitive HSC emergence within the embryo on E10.5. Here, we have shown the existence of T cell-restricted progenitors in the E9.5 YS that directly engraft in recipient immunodeficient mice. T-cell progenitors were also produced in vitro from both YS and para-aortic splanchnopleura hemogenic endothelial cells, and these T-cell progenitors repopulated the thymus and differentiated into mature T-cell subsets in vivo on transplantation. Our data confirm that the YS produces T-lineage-restricted progenitors that are available to colonize the thymus and provide new insight into the YS as a definitive hematopoietic site in the mouse embryo.

Original languageEnglish (US)
Pages (from-to)5706-5714
Number of pages9
JournalBlood
Volume119
Issue number24
DOIs
StatePublished - Jun 14 2012

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Yolk Sac
T-cells
T-Lymphocytes
Thymus
Embryonic Structures
Thymus Gland
Hemangioblasts
Endothelial cells
Erythroid Precursor Cells
T-Lymphocyte Subsets
Transplantation

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Autonomous murine T-cell progenitor production in the extra-embryonic yolk sac before HSC emergence. / Yoshimoto, Momoko; Porayette, Prashanth; Glosson, Nicole L.; Conway, Simon J.; Carlesso, Nadia; Cardoso, Angelo A.; Kaplan, Mark H.; Yoder, Mervin C.

In: Blood, Vol. 119, No. 24, 14.06.2012, p. 5706-5714.

Research output: Contribution to journalArticle

Yoshimoto, Momoko ; Porayette, Prashanth ; Glosson, Nicole L. ; Conway, Simon J. ; Carlesso, Nadia ; Cardoso, Angelo A. ; Kaplan, Mark H. ; Yoder, Mervin C. / Autonomous murine T-cell progenitor production in the extra-embryonic yolk sac before HSC emergence. In: Blood. 2012 ; Vol. 119, No. 24. pp. 5706-5714.
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AU - Carlesso, Nadia

AU - Cardoso, Angelo A.

AU - Kaplan, Mark H.

AU - Yoder, Mervin C.

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