Autoradiographic demonstration of high affinity nuclear binding and finite binding capacity of [3H]estradiol in mouse vaginal cells

Gerald R. Cunha, John M. Shannon, Ken D. Vanderslice, Kathleen McCormick, Robert M. Bigsby

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Vaginae from adult C57B1/6J mice were analyzed for nuclear estrogen-binding sites by biochemical as well as in vitro steroid autoradiographic methods. Finite binding capacity (saturability) and high affinity binding were demonstrated in stromal cell nuclei (Kd = 1.0 nM) by autoradiographic methods and in nuclear extracts of vaginal homogenates (Kd = 1.9 nM) by biochemical techniques. The results of this study demonstrate that all criteria considered definitive for estrogen receptors can be met by autoradiographic analysis, which makes feasible the assessment of estrogen receptor activity in the individual cell types that comprise fetal and neonatal estrogen target organs.

Original languageEnglish (US)
Pages (from-to)1427-1430
Number of pages4
Issue number4
StatePublished - Oct 1983


ASJC Scopus subject areas

  • Endocrinology

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