Autoradiographic demonstration of high affinity nuclear binding and finite binding capacity of [3H]estradiol in mouse vaginal cells

G. R. Cunha, J. M. Shannon, K. D. Vanderslice, K. McCormick, Robert Bigsby

Research output: Contribution to journalArticle

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Abstract

Vaginae from adult C57Bl/6J mice were analyzed for nuclear estrogen-binding sites by biochemical as well as in vitro steroid autoradiographic methods. Finite binding capacity (saturability) and high affinity binding were demonstrated in stromal cell nuclei (K(d) = 1.0nM) by autoradiographic methods and in nuclear extracts of vaginal homogenates (K(d) = 1.9 nM) by biochemical techniques. The results of this study demonstrate that all criteria considered definitive for estrogen receptors can be met by autoradiographic analysis, which makes feasible the assessment of estrogen receptor activity in the individual cell types that comprise fetal and neonatal estrogen target organs.

Original languageEnglish (US)
Pages (from-to)1427-1430
Number of pages4
JournalEndocrinology
Volume113
Issue number4
StatePublished - 1983
Externally publishedYes

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Estrogen Receptors
Estradiol
Estrogens
Vagina
Stromal Cells
Cell Nucleus
Steroids
Binding Sites
In Vitro Techniques

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Autoradiographic demonstration of high affinity nuclear binding and finite binding capacity of [3H]estradiol in mouse vaginal cells. / Cunha, G. R.; Shannon, J. M.; Vanderslice, K. D.; McCormick, K.; Bigsby, Robert.

In: Endocrinology, Vol. 113, No. 4, 1983, p. 1427-1430.

Research output: Contribution to journalArticle

Cunha, G. R. ; Shannon, J. M. ; Vanderslice, K. D. ; McCormick, K. ; Bigsby, Robert. / Autoradiographic demonstration of high affinity nuclear binding and finite binding capacity of [3H]estradiol in mouse vaginal cells. In: Endocrinology. 1983 ; Vol. 113, No. 4. pp. 1427-1430.
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AU - McCormick, K.

AU - Bigsby, Robert

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