Autoradiographic localization of leptin binding in the choroid plexus of ob/ob and db/db mice

Richard B. Lynn, Gao Yuan Cao, Robert Considine, Thomas M. Hyde, Jose F. Caro

Research output: Contribution to journalArticle

109 Citations (Scopus)

Abstract

The obese gene product, leptin, is synthesized in adipose tissue and is a circulating factor regulating body weight. To identify the location of leptin receptors in the brain we have performed an autoradiographic study of the binding of [125I]leptin to frozen sections of mouse brain. Dense specific binding of [125I]leptin was found only in the choroid plexus which is located in the dorsal part of the third ventricle and lateral ventricles. Specific binding of [125I]leptin was found in the ob/ob and db/db mice. These findings further our understanding of the sites and mechanism of action of leptin on brain centers regulating body weight.

Original languageEnglish (US)
Pages (from-to)884-889
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume219
Issue number3
DOIs
StatePublished - Feb 27 1996
Externally publishedYes

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Choroid Plexus
Leptin
Brain
Body Weight
Leptin Receptors
Third Ventricle
Lateral Ventricles
Frozen Sections
Adipose Tissue
Tissue

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Autoradiographic localization of leptin binding in the choroid plexus of ob/ob and db/db mice. / Lynn, Richard B.; Cao, Gao Yuan; Considine, Robert; Hyde, Thomas M.; Caro, Jose F.

In: Biochemical and Biophysical Research Communications, Vol. 219, No. 3, 27.02.1996, p. 884-889.

Research output: Contribution to journalArticle

Lynn, Richard B. ; Cao, Gao Yuan ; Considine, Robert ; Hyde, Thomas M. ; Caro, Jose F. / Autoradiographic localization of leptin binding in the choroid plexus of ob/ob and db/db mice. In: Biochemical and Biophysical Research Communications. 1996 ; Vol. 219, No. 3. pp. 884-889.
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