Autoreactivity to glucose regulated protein 78 links emphysema and osteoporosis in smokers

Jessica Bon, Rehan Kahloon, Yingze Zhang, Jianmin Xue, Carl R. Fuhrman, Jiangning Tan, Mathew Burger, Daniel J. Kass, Eva Csizmadia, Leo Otterbein, Divay Chandra, Arpit Bhargava, Joseph M. Pilewski, G. David Roodman, Frank C. Sciurba, Steven R. Duncan

Research output: Contribution to journalArticle

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Abstract

RATIONALE: Emphysema and osteoporosis are epidemiologically associated diseases of cigarette smokers. The causal mechanism(s) linking these illnesses is unknown. We hypothesized autoimmune responses may be involved in both disorders.

OBJECTIVES: To discover an antigen-specific autoimmune response associated with both emphysema and osteoporosis among smokers.

METHODS: Replicate nonbiased discovery assays indicated that autoimmunity to glucose regulated protein 78 (GRP78), an endoplasmic reticulum chaperone and cell surface signaling receptor, is present in many smokers. Subject assessments included spirometry, chest CT scans, dual x-ray absorptiometry, and immunoblots for anti-GRP78 IgG. Anti-GRP78 autoantibodies were isolated from patient plasma by affinity chromatography, leukocyte functions assessed by flow cytometry, and soluble metabolites and mediators measured by immunoassays.

MEASUREMENTS AND MAIN RESULTS: Circulating anti-GRP78 IgG autoantibodies were detected in plasma specimens from 86 (32%) of the 265 smoking subjects. Anti-GRP78 autoantibodies were singularly prevalent among subjects with radiographic emphysema (OR 3.1, 95%CI 1.7-5.7, p = 0.003). Anti-GRP78 autoantibodies were also associated with osteoporosis (OR 4.7, 95%CI 1.7-13.3, p = 0.002), and increased circulating bone metabolites (p = 0.006). Among emphysematous subjects, GRP78 protein was an autoantigen of CD4 T-cells, stimulating lymphocyte proliferation (p = 0.0002) and IFN-gamma production (p = 0.03). Patient-derived anti-GRP78 autoantibodies had avidities for osteoclasts and macrophages, and increased macrophage NFkB phosphorylation (p = 0.005) and productions of IL-8, CCL-2, and MMP9 (p = 0.005, 0.007, 0.03, respectively).

CONCLUSIONS: Humoral and cellular GRP78 autoimmune responses in smokers have numerous biologically-relevant pro-inflammatory and other deleterious actions, and are associated with emphysema and osteoporosis. These findings may have relevance for the pathogenesis of smoking-associated diseases, and development of biomarker immunoassays and/or novel treatments for these disorders.

Original languageEnglish
Pages (from-to)e105066
JournalPLoS One
Volume9
Issue number9
DOIs
StatePublished - 2014

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osteoporosis
Emphysema
Osteoporosis
glucose
autoantibodies
Autoantibodies
autoimmunity
Autoimmunity
proteins
Macrophages
Metabolites
Immunoassay
immunoassays
Smoking
macrophages
Plasmas
Affinity chromatography
glucose-regulated proteins
metabolites
autoantigens

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Bon, J., Kahloon, R., Zhang, Y., Xue, J., Fuhrman, C. R., Tan, J., ... Duncan, S. R. (2014). Autoreactivity to glucose regulated protein 78 links emphysema and osteoporosis in smokers. PLoS One, 9(9), e105066. https://doi.org/10.1371/journal.pone.0105066

Autoreactivity to glucose regulated protein 78 links emphysema and osteoporosis in smokers. / Bon, Jessica; Kahloon, Rehan; Zhang, Yingze; Xue, Jianmin; Fuhrman, Carl R.; Tan, Jiangning; Burger, Mathew; Kass, Daniel J.; Csizmadia, Eva; Otterbein, Leo; Chandra, Divay; Bhargava, Arpit; Pilewski, Joseph M.; Roodman, G. David; Sciurba, Frank C.; Duncan, Steven R.

In: PLoS One, Vol. 9, No. 9, 2014, p. e105066.

Research output: Contribution to journalArticle

Bon, J, Kahloon, R, Zhang, Y, Xue, J, Fuhrman, CR, Tan, J, Burger, M, Kass, DJ, Csizmadia, E, Otterbein, L, Chandra, D, Bhargava, A, Pilewski, JM, Roodman, GD, Sciurba, FC & Duncan, SR 2014, 'Autoreactivity to glucose regulated protein 78 links emphysema and osteoporosis in smokers', PLoS One, vol. 9, no. 9, pp. e105066. https://doi.org/10.1371/journal.pone.0105066
Bon, Jessica ; Kahloon, Rehan ; Zhang, Yingze ; Xue, Jianmin ; Fuhrman, Carl R. ; Tan, Jiangning ; Burger, Mathew ; Kass, Daniel J. ; Csizmadia, Eva ; Otterbein, Leo ; Chandra, Divay ; Bhargava, Arpit ; Pilewski, Joseph M. ; Roodman, G. David ; Sciurba, Frank C. ; Duncan, Steven R. / Autoreactivity to glucose regulated protein 78 links emphysema and osteoporosis in smokers. In: PLoS One. 2014 ; Vol. 9, No. 9. pp. e105066.
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abstract = "RATIONALE: Emphysema and osteoporosis are epidemiologically associated diseases of cigarette smokers. The causal mechanism(s) linking these illnesses is unknown. We hypothesized autoimmune responses may be involved in both disorders.OBJECTIVES: To discover an antigen-specific autoimmune response associated with both emphysema and osteoporosis among smokers.METHODS: Replicate nonbiased discovery assays indicated that autoimmunity to glucose regulated protein 78 (GRP78), an endoplasmic reticulum chaperone and cell surface signaling receptor, is present in many smokers. Subject assessments included spirometry, chest CT scans, dual x-ray absorptiometry, and immunoblots for anti-GRP78 IgG. Anti-GRP78 autoantibodies were isolated from patient plasma by affinity chromatography, leukocyte functions assessed by flow cytometry, and soluble metabolites and mediators measured by immunoassays.MEASUREMENTS AND MAIN RESULTS: Circulating anti-GRP78 IgG autoantibodies were detected in plasma specimens from 86 (32{\%}) of the 265 smoking subjects. Anti-GRP78 autoantibodies were singularly prevalent among subjects with radiographic emphysema (OR 3.1, 95{\%}CI 1.7-5.7, p = 0.003). Anti-GRP78 autoantibodies were also associated with osteoporosis (OR 4.7, 95{\%}CI 1.7-13.3, p = 0.002), and increased circulating bone metabolites (p = 0.006). Among emphysematous subjects, GRP78 protein was an autoantigen of CD4 T-cells, stimulating lymphocyte proliferation (p = 0.0002) and IFN-gamma production (p = 0.03). Patient-derived anti-GRP78 autoantibodies had avidities for osteoclasts and macrophages, and increased macrophage NFkB phosphorylation (p = 0.005) and productions of IL-8, CCL-2, and MMP9 (p = 0.005, 0.007, 0.03, respectively).CONCLUSIONS: Humoral and cellular GRP78 autoimmune responses in smokers have numerous biologically-relevant pro-inflammatory and other deleterious actions, and are associated with emphysema and osteoporosis. These findings may have relevance for the pathogenesis of smoking-associated diseases, and development of biomarker immunoassays and/or novel treatments for these disorders.",
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T1 - Autoreactivity to glucose regulated protein 78 links emphysema and osteoporosis in smokers

AU - Bon, Jessica

AU - Kahloon, Rehan

AU - Zhang, Yingze

AU - Xue, Jianmin

AU - Fuhrman, Carl R.

AU - Tan, Jiangning

AU - Burger, Mathew

AU - Kass, Daniel J.

AU - Csizmadia, Eva

AU - Otterbein, Leo

AU - Chandra, Divay

AU - Bhargava, Arpit

AU - Pilewski, Joseph M.

AU - Roodman, G. David

AU - Sciurba, Frank C.

AU - Duncan, Steven R.

PY - 2014

Y1 - 2014

N2 - RATIONALE: Emphysema and osteoporosis are epidemiologically associated diseases of cigarette smokers. The causal mechanism(s) linking these illnesses is unknown. We hypothesized autoimmune responses may be involved in both disorders.OBJECTIVES: To discover an antigen-specific autoimmune response associated with both emphysema and osteoporosis among smokers.METHODS: Replicate nonbiased discovery assays indicated that autoimmunity to glucose regulated protein 78 (GRP78), an endoplasmic reticulum chaperone and cell surface signaling receptor, is present in many smokers. Subject assessments included spirometry, chest CT scans, dual x-ray absorptiometry, and immunoblots for anti-GRP78 IgG. Anti-GRP78 autoantibodies were isolated from patient plasma by affinity chromatography, leukocyte functions assessed by flow cytometry, and soluble metabolites and mediators measured by immunoassays.MEASUREMENTS AND MAIN RESULTS: Circulating anti-GRP78 IgG autoantibodies were detected in plasma specimens from 86 (32%) of the 265 smoking subjects. Anti-GRP78 autoantibodies were singularly prevalent among subjects with radiographic emphysema (OR 3.1, 95%CI 1.7-5.7, p = 0.003). Anti-GRP78 autoantibodies were also associated with osteoporosis (OR 4.7, 95%CI 1.7-13.3, p = 0.002), and increased circulating bone metabolites (p = 0.006). Among emphysematous subjects, GRP78 protein was an autoantigen of CD4 T-cells, stimulating lymphocyte proliferation (p = 0.0002) and IFN-gamma production (p = 0.03). Patient-derived anti-GRP78 autoantibodies had avidities for osteoclasts and macrophages, and increased macrophage NFkB phosphorylation (p = 0.005) and productions of IL-8, CCL-2, and MMP9 (p = 0.005, 0.007, 0.03, respectively).CONCLUSIONS: Humoral and cellular GRP78 autoimmune responses in smokers have numerous biologically-relevant pro-inflammatory and other deleterious actions, and are associated with emphysema and osteoporosis. These findings may have relevance for the pathogenesis of smoking-associated diseases, and development of biomarker immunoassays and/or novel treatments for these disorders.

AB - RATIONALE: Emphysema and osteoporosis are epidemiologically associated diseases of cigarette smokers. The causal mechanism(s) linking these illnesses is unknown. We hypothesized autoimmune responses may be involved in both disorders.OBJECTIVES: To discover an antigen-specific autoimmune response associated with both emphysema and osteoporosis among smokers.METHODS: Replicate nonbiased discovery assays indicated that autoimmunity to glucose regulated protein 78 (GRP78), an endoplasmic reticulum chaperone and cell surface signaling receptor, is present in many smokers. Subject assessments included spirometry, chest CT scans, dual x-ray absorptiometry, and immunoblots for anti-GRP78 IgG. Anti-GRP78 autoantibodies were isolated from patient plasma by affinity chromatography, leukocyte functions assessed by flow cytometry, and soluble metabolites and mediators measured by immunoassays.MEASUREMENTS AND MAIN RESULTS: Circulating anti-GRP78 IgG autoantibodies were detected in plasma specimens from 86 (32%) of the 265 smoking subjects. Anti-GRP78 autoantibodies were singularly prevalent among subjects with radiographic emphysema (OR 3.1, 95%CI 1.7-5.7, p = 0.003). Anti-GRP78 autoantibodies were also associated with osteoporosis (OR 4.7, 95%CI 1.7-13.3, p = 0.002), and increased circulating bone metabolites (p = 0.006). Among emphysematous subjects, GRP78 protein was an autoantigen of CD4 T-cells, stimulating lymphocyte proliferation (p = 0.0002) and IFN-gamma production (p = 0.03). Patient-derived anti-GRP78 autoantibodies had avidities for osteoclasts and macrophages, and increased macrophage NFkB phosphorylation (p = 0.005) and productions of IL-8, CCL-2, and MMP9 (p = 0.005, 0.007, 0.03, respectively).CONCLUSIONS: Humoral and cellular GRP78 autoimmune responses in smokers have numerous biologically-relevant pro-inflammatory and other deleterious actions, and are associated with emphysema and osteoporosis. These findings may have relevance for the pathogenesis of smoking-associated diseases, and development of biomarker immunoassays and/or novel treatments for these disorders.

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