Axonal transport of British and Danish amyloid peptides via secretory vesicles.

Seung Il Choi, Ruben Vidal, Blas Frangione, Efrat Levy

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The ABri and ADan amyloid peptides deposited in familial British and Danish neurodegenerative disorders are generated by processing mutant forms of the precursor protein BRI2. Although the pathogenic process that leads to deposition of amyloid in the brains of patients has been studied extensively, the cellular processes and normal function of the precursor protein did not receive much attention. We observed in a variety of transfected cell lines the presence of two independent proteolytic processing events. In addition to the previously described cleavage, which results in the production of carboxyl-terminal approximately 3 kDa wild-type peptide or approximately 4 kDa ABri or ADan peptides, we describe a novel amino-terminal cleavage site within BRI2. Both cleavages occur within the cis- or medial-Golgi. Following cleavage, the BRI2-derived carboxyl-terminal peptides are transported via a regulated secretory pathway into secretory vesicles in neuronal cells. Worth noting is that expression of wild-type British or Danish mutants of BRI2, in mouse neuroblastoma N2a cells that do not express endogenous BRI2, induces elongation of neurites, which suggests a role for this protein in differentiation of neuronal cells.

Original languageEnglish (US)
Pages (from-to)373-375
Number of pages3
JournalFASEB Journal
Volume18
Issue number2
StatePublished - 2004
Externally publishedYes

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Axonal Transport
Secretory Vesicles
Amyloid
Abrus
Peptides
Protein Precursors
Cells
Secretory Pathway
Neurites
Processing
Neuroblastoma
Neurodegenerative Diseases
Cell Differentiation
Elongation
Brain
Cell Line
Proteins

Cite this

Axonal transport of British and Danish amyloid peptides via secretory vesicles. / Choi, Seung Il; Vidal, Ruben; Frangione, Blas; Levy, Efrat.

In: FASEB Journal, Vol. 18, No. 2, 2004, p. 373-375.

Research output: Contribution to journalArticle

Choi, SI, Vidal, R, Frangione, B & Levy, E 2004, 'Axonal transport of British and Danish amyloid peptides via secretory vesicles.', FASEB Journal, vol. 18, no. 2, pp. 373-375.
Choi, Seung Il ; Vidal, Ruben ; Frangione, Blas ; Levy, Efrat. / Axonal transport of British and Danish amyloid peptides via secretory vesicles. In: FASEB Journal. 2004 ; Vol. 18, No. 2. pp. 373-375.
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