Azidoacridines as photoaffinity probes for ionic channels in excitable membranes

Gerry S. Oxford, Richard A. Hudson

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Quinacrine and a photoactivatable congener, quinacrine azide, were applied to the intracellular membrane surface of voltage-clamped squid giant axons using internal perfusion techniques. Both compounds were found to reversibly block voltage-dependent sodium channels under dark conditions. Potassium channels were blocked to a lesser extent. Upon irradiation an irreversible block of sodium channels developed with quinacrine azide, but not with quinacrine. Quinacrine azide may thus represent a class of useful photoaffinity probes of voltage-dependent ionic channels.

Original languageEnglish (US)
Pages (from-to)1579-1584
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume104
Issue number4
DOIs
StatePublished - Feb 26 1982
Externally publishedYes

Fingerprint

Ion Channels
Quinacrine
Sodium Channels
Membranes
Electric potential
Decapodiformes
Intracellular Membranes
Potassium Channels
Axons
Perfusion
Irradiation
quinacrine azide

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Azidoacridines as photoaffinity probes for ionic channels in excitable membranes. / Oxford, Gerry S.; Hudson, Richard A.

In: Biochemical and Biophysical Research Communications, Vol. 104, No. 4, 26.02.1982, p. 1579-1584.

Research output: Contribution to journalArticle

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