B cell subpopulations separated by CD27 and crucial collaboration of CD27 + B cells and helper T cells in immunoglobulin production

Kazunaga Agematsu, Haruo Nagumo, Fen Chun Yang, Takayuki Nakazawa, Keitaro Fukushima, Susumu Ito, Kanji Sugita, Tetsuo Mori, Tetsuji Kobata, Chikao Morimoto, Atsushi Komiyama

Research output: Contribution to journalArticle

225 Citations (Scopus)

Abstract

B cell immunoglobulin production is regulated by helper T cells through direct interaction and secreted cytokines. In the present study, we functionally analyzed CD27 in cord and peripheral blood B cells. Adult peripheral blood B cells were separated into CD27 + and CD27 - cells, which differed in their morphology. Cord blood B cells did not express CD27, and CD27 expression on peripheral blood B cells increased with age. Only CD27 + B cells had the ability to produce immunoglobulin, which was increased by contact with a tumor necrosis factor-related transmembrane ligand, CD70. Adult peripheral blood CD27 + B cells can be further subdivided into two discrete subtypes: IgD- CD27 + and IgD + CD27 + B cells. IgD- CD27 + B cells produce IgG, IgM and IgA, whereas IgD + CD27 + B cells predominantly produce IgM. The addition of activated CD4 +CD45RO T cells expressing CD70 caused down-regulation of CD27 expression on activated B cells, and this down-modulation was completely blocked by anti-CD70 monoclonal antibody, indicating direct T-B cell contact via CD27/CD70. The triggering via CD27 and CD40 additively increased the immunoglobulin production under Staphylococcus aureus Cowan strain plus interleukin-2 stimulation. Taken together, our findings demonstrate that peripheral blood B cells are separated into subpopulations by CD27 and IgD expression and that CD27 + B cells produce large amounts of immunoglobulin by interaction with the CD70 molecule.

Original languageEnglish (US)
Pages (from-to)2073-2079
Number of pages7
JournalEuropean Journal of Immunology
Volume27
Issue number8
DOIs
StatePublished - Aug 1997
Externally publishedYes

Fingerprint

Helper-Inducer T-Lymphocytes
Immunoglobulins
B-Lymphocytes
Immunoglobulin D
Blood Cells
Fetal Blood
Immunoglobulin M
Immunoglobulin A
Interleukin-2
Staphylococcus aureus
Down-Regulation
Tumor Necrosis Factor-alpha
Immunoglobulin G
Monoclonal Antibodies

Keywords

  • CD27
  • Human
  • IgD
  • T cell-B cell collaboration

ASJC Scopus subject areas

  • Immunology

Cite this

B cell subpopulations separated by CD27 and crucial collaboration of CD27 + B cells and helper T cells in immunoglobulin production. / Agematsu, Kazunaga; Nagumo, Haruo; Yang, Fen Chun; Nakazawa, Takayuki; Fukushima, Keitaro; Ito, Susumu; Sugita, Kanji; Mori, Tetsuo; Kobata, Tetsuji; Morimoto, Chikao; Komiyama, Atsushi.

In: European Journal of Immunology, Vol. 27, No. 8, 08.1997, p. 2073-2079.

Research output: Contribution to journalArticle

Agematsu, K, Nagumo, H, Yang, FC, Nakazawa, T, Fukushima, K, Ito, S, Sugita, K, Mori, T, Kobata, T, Morimoto, C & Komiyama, A 1997, 'B cell subpopulations separated by CD27 and crucial collaboration of CD27 + B cells and helper T cells in immunoglobulin production', European Journal of Immunology, vol. 27, no. 8, pp. 2073-2079. https://doi.org/10.1002/eji.1830270835
Agematsu, Kazunaga ; Nagumo, Haruo ; Yang, Fen Chun ; Nakazawa, Takayuki ; Fukushima, Keitaro ; Ito, Susumu ; Sugita, Kanji ; Mori, Tetsuo ; Kobata, Tetsuji ; Morimoto, Chikao ; Komiyama, Atsushi. / B cell subpopulations separated by CD27 and crucial collaboration of CD27 + B cells and helper T cells in immunoglobulin production. In: European Journal of Immunology. 1997 ; Vol. 27, No. 8. pp. 2073-2079.
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