Bacterial communities of the coronal sulcus and distal urethra of adolescent males

David E. Nelson, Qunfeng Dong, Barbara van der Pol, Evelyn Toh, Baochang Fan, Barry Katz, Deming Mi, Ruichen Rong, George M. Weinstock, Erica Sodergren, J. Fortenberry

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Lactobacillus-dominated vaginal microbiotas are associated with reproductive health and STI resistance in women, whereas altered microbiotas are associated with bacterial vaginosis (BV), STI risk and poor reproductive outcomes. Putative vaginal taxa have been observed in male first-catch urine, urethral swab and coronal sulcus (CS) specimens but the significance of these observations is unclear. We used 16 S rRNA sequencing to characterize the microbiota of the CS and urine collected from 18 adolescent men over three consecutive months. CS microbiotas of most participants were more stable than their urine microbiotas and the composition of CS microbiotas were strongly influenced by circumcision. BV-associated taxa, including Atopobium, Megasphaera, Mobiluncus, Prevotella and Gemella, were detected in CS specimens from sexually experienced and inexperienced participants. In contrast, urine primarily contained taxa that were not abundant in CS specimens. Lactobacilllus and Streptococcus were major urine taxa but their abundance was inversely correlated. In contrast, Sneathia, Mycoplasma and Ureaplasma were only found in urine from sexually active participants. Thus, the CS and urine support stable and distinct bacterial communities. Finally, our results suggest that the penis and the urethra can be colonized by a variety of BV-associated taxa and that some of these colonizations result from partnered sexual activity.

Original languageEnglish
Article numbere36298
JournalPLoS One
Volume7
Issue number5
DOIs
StatePublished - May 11 2012

Fingerprint

urethra
Urethra
bacterial communities
Microbiota
urine
Health
Urine
Bacterial Vaginosis
Chemical analysis
Sexually Transmitted Diseases
Mobiluncus
Atopobium
Gemella
Megasphaera
Prevotella
Ureaplasma
Mycoplasma
penis
Reproductive Health
Streptococcus

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Bacterial communities of the coronal sulcus and distal urethra of adolescent males. / Nelson, David E.; Dong, Qunfeng; van der Pol, Barbara; Toh, Evelyn; Fan, Baochang; Katz, Barry; Mi, Deming; Rong, Ruichen; Weinstock, George M.; Sodergren, Erica; Fortenberry, J.

In: PLoS One, Vol. 7, No. 5, e36298, 11.05.2012.

Research output: Contribution to journalArticle

Nelson, DE, Dong, Q, van der Pol, B, Toh, E, Fan, B, Katz, B, Mi, D, Rong, R, Weinstock, GM, Sodergren, E & Fortenberry, J 2012, 'Bacterial communities of the coronal sulcus and distal urethra of adolescent males', PLoS One, vol. 7, no. 5, e36298. https://doi.org/10.1371/journal.pone.0036298
Nelson, David E. ; Dong, Qunfeng ; van der Pol, Barbara ; Toh, Evelyn ; Fan, Baochang ; Katz, Barry ; Mi, Deming ; Rong, Ruichen ; Weinstock, George M. ; Sodergren, Erica ; Fortenberry, J. / Bacterial communities of the coronal sulcus and distal urethra of adolescent males. In: PLoS One. 2012 ; Vol. 7, No. 5.
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