Bacterial growth in space flight: Logistic growth curve parameters for Escherichia coli and Bacillus subtilis

Melissa Kacena, G. A. Merrell, B. Manfredi, E. E. Smith, D. M. Klaus, P. Todd

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

Previous investigations have reported that bacterial suspension cultures grow to higher stationary concentrations in space flight than on Earth; however, none of these investigations included extensive ground controls under varied inertial conditions. This study includes extensive controls and cell-growth data taken at several times during lag phase, log phase, and stationary phase of Escherichia coli and Bacillus subtilis. The Marquardt- Levenberg, least-squares fitting algorithm was used to calculate kinetic growth parameters from the logistic bacterial growth equations for space- flight and control growth curves. Space-flight cultures grew to higher stationary-phase concentrations and had shorter lag-phase durations. Also, evidence was found for increased exponential growth rate in space.

Original languageEnglish (US)
Pages (from-to)229-234
Number of pages6
JournalApplied Microbiology and Biotechnology
Volume51
Issue number2
DOIs
StatePublished - 1999
Externally publishedYes

Fingerprint

Space Flight
Bacilli
Space flight
Bacillus subtilis
Escherichia coli
Logistics
Growth
Growth kinetics
Cell growth
Suspensions
Earth (planet)
Least-Squares Analysis

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology
  • Bioengineering

Cite this

Bacterial growth in space flight : Logistic growth curve parameters for Escherichia coli and Bacillus subtilis. / Kacena, Melissa; Merrell, G. A.; Manfredi, B.; Smith, E. E.; Klaus, D. M.; Todd, P.

In: Applied Microbiology and Biotechnology, Vol. 51, No. 2, 1999, p. 229-234.

Research output: Contribution to journalArticle

Kacena, Melissa ; Merrell, G. A. ; Manfredi, B. ; Smith, E. E. ; Klaus, D. M. ; Todd, P. / Bacterial growth in space flight : Logistic growth curve parameters for Escherichia coli and Bacillus subtilis. In: Applied Microbiology and Biotechnology. 1999 ; Vol. 51, No. 2. pp. 229-234.
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