BATF transgenic mice reveal a role for activator protein-1 in NKT cell development

Kristi L. Williams, Alfred J. Zullo, Mark Kaplan, Randy Brutkiewicz, Christopher D. Deppmann, Charles Vinson, Elizabeth J. Taparowsky

Research output: Contribution to journalArticle

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Abstract

The importance of regulated AP-1 activity during T cell development was assessed using transgenic mice overexpressing BATF, a basic leucine zipper transcription factor and an AP-1 inhibitor. BATF transgenic animals possess normal thymic cellularity and all major T cell subsets, but show impaired thymocyte proliferation in vitro and no induction of IL-2, IL-4, IL-5, IL-10, and IL-13 expression. Since NKT cells are largely responsible for cytokine production in the thymus, this population was examined by detection of the Vα14-Jα281 TCR, flow cytometry of NK1.1+ TCRβ+ cells, and analysis of cytokine production by heat-stable Aglow thymocytes and peripheral NKT cells stimulated in vivo. Results show a severe under-representation of NKT cells in BATF transgenic animals, providing the first evidence that the precise control of AP-1-mediated transcription is critical for the proper emergence of thymus-derived NKT cells in the mouse.

Original languageEnglish
Pages (from-to)2417-2426
Number of pages10
JournalJournal of Immunology
Volume170
Issue number5
StatePublished - Mar 1 2003

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Natural Killer T-Cells
Transcription Factor AP-1
Transgenic Mice
Genetically Modified Animals
Thymocytes
Thymus Gland
Basic-Leucine Zipper Transcription Factors
Cytokines
Interleukin-13
Thermogenesis
Interleukin-5
T-Lymphocyte Subsets
Interleukin-4
Interleukin-10
Interleukin-2
Flow Cytometry
T-Lymphocytes
Population

ASJC Scopus subject areas

  • Immunology

Cite this

Williams, K. L., Zullo, A. J., Kaplan, M., Brutkiewicz, R., Deppmann, C. D., Vinson, C., & Taparowsky, E. J. (2003). BATF transgenic mice reveal a role for activator protein-1 in NKT cell development. Journal of Immunology, 170(5), 2417-2426.

BATF transgenic mice reveal a role for activator protein-1 in NKT cell development. / Williams, Kristi L.; Zullo, Alfred J.; Kaplan, Mark; Brutkiewicz, Randy; Deppmann, Christopher D.; Vinson, Charles; Taparowsky, Elizabeth J.

In: Journal of Immunology, Vol. 170, No. 5, 01.03.2003, p. 2417-2426.

Research output: Contribution to journalArticle

Williams, KL, Zullo, AJ, Kaplan, M, Brutkiewicz, R, Deppmann, CD, Vinson, C & Taparowsky, EJ 2003, 'BATF transgenic mice reveal a role for activator protein-1 in NKT cell development', Journal of Immunology, vol. 170, no. 5, pp. 2417-2426.
Williams, Kristi L. ; Zullo, Alfred J. ; Kaplan, Mark ; Brutkiewicz, Randy ; Deppmann, Christopher D. ; Vinson, Charles ; Taparowsky, Elizabeth J. / BATF transgenic mice reveal a role for activator protein-1 in NKT cell development. In: Journal of Immunology. 2003 ; Vol. 170, No. 5. pp. 2417-2426.
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