BATF transgenic mice reveal a role for activator protein-1 in NKT cell development

Kristi L. Williams, Alfred J. Zullo, Mark H. Kaplan, Randy R. Brutkiewicz, Christopher D. Deppmann, Charles Vinson, Elizabeth J. Taparowsky

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

The importance of regulated AP-1 activity during T cell development was assessed using transgenic mice overexpressing BATF, a basic leucine zipper transcription factor and an AP-1 inhibitor. BATF transgenic animals possess normal thymic cellularity and all major T cell subsets, but show impaired thymocyte proliferation in vitro and no induction of IL-2, IL-4, IL-5, IL-10, and IL-13 expression. Since NKT cells are largely responsible for cytokine production in the thymus, this population was examined by detection of the Vα14-Jα281 TCR, flow cytometry of NK1.1+ TCRβ+ cells, and analysis of cytokine production by heat-stable Aglow thymocytes and peripheral NKT cells stimulated in vivo. Results show a severe under-representation of NKT cells in BATF transgenic animals, providing the first evidence that the precise control of AP-1-mediated transcription is critical for the proper emergence of thymus-derived NKT cells in the mouse.

Original languageEnglish (US)
Pages (from-to)2417-2426
Number of pages10
JournalJournal of Immunology
Volume170
Issue number5
DOIs
StatePublished - Mar 1 2003

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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