Bcl6 and Blimp-1 are reciprocal and antagonistic regulators of T follicular helper cell differentiation

Robert J. Johnston, Amanda C. Poholek, Daniel DiToro, Isharat Yusuf, Danelle Eto, Burton Barnett, Alexander Dent, Joe Craft, Shane Crotty

Research output: Contribution to journalArticle

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Abstract

Effective B cell-mediated immunity and antibody responses often require help from CD4+ T cells. It is thought that a distinct CD4+ effector T cell subset, called T follicular helper cells (TFH), provides this help; however, the molecular requirements for TFH differentiation are unknown. We found that expression of the transcription factor Bcl6 in CD4 + T cells is both necessary and sufficient for in vivo TFH differentiation and T cell help to B cells in mice. In contrast, the transcription factor Blimp-1, an antagonist of Bcl6, inhibits TFH differentiation and help, thereby preventing B cell germinal center and antibody responses. These findings demonstrate that TFH cells are required for proper B cell responses in vivo and that Bcl6 and Blimp-1 play central but opposing roles in TFH differentiation.

Original languageEnglish
Pages (from-to)1006-1010
Number of pages5
JournalScience
Volume325
Issue number5943
DOIs
StatePublished - 2009

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Cell Differentiation
B-Lymphocytes
Helper-Inducer T-Lymphocytes
T-Lymphocytes
Antibody Formation
Transcription Factors
Germinal Center
T-Lymphocyte Subsets
Cellular Immunity

ASJC Scopus subject areas

  • General

Cite this

Johnston, R. J., Poholek, A. C., DiToro, D., Yusuf, I., Eto, D., Barnett, B., ... Crotty, S. (2009). Bcl6 and Blimp-1 are reciprocal and antagonistic regulators of T follicular helper cell differentiation. Science, 325(5943), 1006-1010. https://doi.org/10.1126/science.1175870

Bcl6 and Blimp-1 are reciprocal and antagonistic regulators of T follicular helper cell differentiation. / Johnston, Robert J.; Poholek, Amanda C.; DiToro, Daniel; Yusuf, Isharat; Eto, Danelle; Barnett, Burton; Dent, Alexander; Craft, Joe; Crotty, Shane.

In: Science, Vol. 325, No. 5943, 2009, p. 1006-1010.

Research output: Contribution to journalArticle

Johnston, RJ, Poholek, AC, DiToro, D, Yusuf, I, Eto, D, Barnett, B, Dent, A, Craft, J & Crotty, S 2009, 'Bcl6 and Blimp-1 are reciprocal and antagonistic regulators of T follicular helper cell differentiation', Science, vol. 325, no. 5943, pp. 1006-1010. https://doi.org/10.1126/science.1175870
Johnston, Robert J. ; Poholek, Amanda C. ; DiToro, Daniel ; Yusuf, Isharat ; Eto, Danelle ; Barnett, Burton ; Dent, Alexander ; Craft, Joe ; Crotty, Shane. / Bcl6 and Blimp-1 are reciprocal and antagonistic regulators of T follicular helper cell differentiation. In: Science. 2009 ; Vol. 325, No. 5943. pp. 1006-1010.
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