OBJECTIVE: To determine whether single nucleotide polymorphisms (SNPs) of the brain-derived neurotrophic factor (BDNF) that have been associated with bipolar illness are associated with physiological dysfunction. Methods: Lymphoblastoid cell lines (n=30) obtained from bipolar I individuals carrying zero, one, or two copies of a BDNF SNP associated with bipolar illness (rs12273363) were utilized. Results: Proapoptotic stressors of serum deprivation alone, or serum deprivation combined with the sodium ionophore, monensin, did not alter intracellular proBDNF. Monensin treatment increased mature-BDNF (mBDNF) protein levels (P<0.05). There were no differences related to the presence of SNP or copy number. Conclusion: rs12273363 does not appear to have functional consequences that would involve its role in bipolar illness.
- bipolar disorder
- brain-derived neurotrophic factor
- intracellular sodium
- single nucleotide polymorphism
ASJC Scopus subject areas
- Psychiatry and Mental health
- Biological Psychiatry
BDNF expression in lymphoblastoid cell lines carrying BDNF SNPs associated with bipolar disorder. / Gao, Yonglin; Galante, Mathew; El-Mallakh, James; Nurnberger, John; Delamere, Nicholas A.; Lei, Zhenmin; El-Mallakh, Rif S.In: Psychiatric Genetics, Vol. 22, No. 5, 10.2012, p. 253-255.
Research output: Contribution to journal › Article
TY - JOUR
T1 - BDNF expression in lymphoblastoid cell lines carrying BDNF SNPs associated with bipolar disorder
AU - Gao, Yonglin
AU - Galante, Mathew
AU - El-Mallakh, James
AU - Nurnberger, John
AU - Delamere, Nicholas A.
AU - Lei, Zhenmin
AU - El-Mallakh, Rif S.
PY - 2012/10
Y1 - 2012/10
N2 - Lippincott Williams & Wilkins.
AB - Lippincott Williams & Wilkins.
KW - apoptosis
KW - bipolar disorder
KW - brain-derived neurotrophic factor
KW - intracellular sodium
KW - single nucleotide polymorphism
UR - http://www.scopus.com/inward/record.url?scp=84865804221&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84865804221&partnerID=8YFLogxK
U2 - 10.1097/YPG.0b013e328353ae66
DO - 10.1097/YPG.0b013e328353ae66
M3 - Article
C2 - 22517381
AN - SCOPUS:84865804221
VL - 22
SP - 253
EP - 255
JO - Psychiatric Genetics
JF - Psychiatric Genetics
SN - 0955-8829
IS - 5