BDNF expression in lymphoblastoid cell lines carrying BDNF SNPs associated with bipolar disorder

Yonglin Gao, Mathew Galante, James El-Mallakh, John Nurnberger, Nicholas A. Delamere, Zhenmin Lei, Rif S. El-Mallakh

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

OBJECTIVE: To determine whether single nucleotide polymorphisms (SNPs) of the brain-derived neurotrophic factor (BDNF) that have been associated with bipolar illness are associated with physiological dysfunction. Methods: Lymphoblastoid cell lines (n=30) obtained from bipolar I individuals carrying zero, one, or two copies of a BDNF SNP associated with bipolar illness (rs12273363) were utilized. Results: Proapoptotic stressors of serum deprivation alone, or serum deprivation combined with the sodium ionophore, monensin, did not alter intracellular proBDNF. Monensin treatment increased mature-BDNF (mBDNF) protein levels (P<0.05). There were no differences related to the presence of SNP or copy number. Conclusion: rs12273363 does not appear to have functional consequences that would involve its role in bipolar illness.

Original languageEnglish
Pages (from-to)253-255
Number of pages3
JournalPsychiatric Genetics
Volume22
Issue number5
DOIs
StatePublished - Oct 2012

Fingerprint

Brain-Derived Neurotrophic Factor
Bipolar Disorder
Single Nucleotide Polymorphism
Monensin
Cell Line
Sodium Ionophores
Nerve Growth Factors
Serum

Keywords

  • apoptosis
  • bipolar disorder
  • brain-derived neurotrophic factor
  • intracellular sodium
  • single nucleotide polymorphism

ASJC Scopus subject areas

  • Genetics(clinical)
  • Psychiatry and Mental health
  • Genetics
  • Biological Psychiatry

Cite this

BDNF expression in lymphoblastoid cell lines carrying BDNF SNPs associated with bipolar disorder. / Gao, Yonglin; Galante, Mathew; El-Mallakh, James; Nurnberger, John; Delamere, Nicholas A.; Lei, Zhenmin; El-Mallakh, Rif S.

In: Psychiatric Genetics, Vol. 22, No. 5, 10.2012, p. 253-255.

Research output: Contribution to journalArticle

Gao, Yonglin ; Galante, Mathew ; El-Mallakh, James ; Nurnberger, John ; Delamere, Nicholas A. ; Lei, Zhenmin ; El-Mallakh, Rif S. / BDNF expression in lymphoblastoid cell lines carrying BDNF SNPs associated with bipolar disorder. In: Psychiatric Genetics. 2012 ; Vol. 22, No. 5. pp. 253-255.
@article{84848d9251cc40f4a4d50faf55054688,
title = "BDNF expression in lymphoblastoid cell lines carrying BDNF SNPs associated with bipolar disorder",
abstract = "Lippincott Williams & Wilkins.",
keywords = "apoptosis, bipolar disorder, brain-derived neurotrophic factor, intracellular sodium, single nucleotide polymorphism",
author = "Yonglin Gao and Mathew Galante and James El-Mallakh and John Nurnberger and Delamere, {Nicholas A.} and Zhenmin Lei and El-Mallakh, {Rif S.}",
year = "2012",
month = "10",
doi = "10.1097/YPG.0b013e328353ae66",
language = "English",
volume = "22",
pages = "253--255",
journal = "Psychiatric Genetics",
issn = "0955-8829",
publisher = "Lippincott Williams and Wilkins",
number = "5",

}

TY - JOUR

T1 - BDNF expression in lymphoblastoid cell lines carrying BDNF SNPs associated with bipolar disorder

AU - Gao, Yonglin

AU - Galante, Mathew

AU - El-Mallakh, James

AU - Nurnberger, John

AU - Delamere, Nicholas A.

AU - Lei, Zhenmin

AU - El-Mallakh, Rif S.

PY - 2012/10

Y1 - 2012/10

N2 - Lippincott Williams & Wilkins.

AB - Lippincott Williams & Wilkins.

KW - apoptosis

KW - bipolar disorder

KW - brain-derived neurotrophic factor

KW - intracellular sodium

KW - single nucleotide polymorphism

UR - http://www.scopus.com/inward/record.url?scp=84865804221&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84865804221&partnerID=8YFLogxK

U2 - 10.1097/YPG.0b013e328353ae66

DO - 10.1097/YPG.0b013e328353ae66

M3 - Article

C2 - 22517381

AN - SCOPUS:84865804221

VL - 22

SP - 253

EP - 255

JO - Psychiatric Genetics

JF - Psychiatric Genetics

SN - 0955-8829

IS - 5

ER -