Benzo(a)pyrene, but not 2,3,7,8-tetrachlorodibenzo-p-dioxin, alters cell adhesion proteins in human uterine RL95-2 cells

Michelle A. McGarry, Grantley D. Charles, Theresa Medrano, Michael R. Bubb, Maria B. Grant, Martha Campbell-Thompson, Kathleen T. Shiverick

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

This study compared the effects of benzo(a)pyrene (BaP) and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), two aryl hydrocarbon receptor agonists, on cell attachment and adherens junction proteins in RL95-2 human uterine endometrial cells. Exposure to 10 μM BaP significantly decreased cell attachment to Matrigel, whereas 10 nM TCDD had no effect. Immunocytochemistry and Western immunoblot analysis showed that BaP, but not TCDD, produced a marked loss of plasma membrane epidermal growth factor receptor (EGF-R) localized along intercellular boundaries. BaP-treated cells exhibited significant decreases in β-catenin and cadherin protein levels, while vinculin levels remained unchanged relative to control. In contrast, TCDD treatment had no effect on the levels of β-catenin, cadherin, or vinculin. Further studies using the fluorescein labeled peptide phalloidin showed the presence of continuous subcortical actin filaments in control cells, whereas BaP-treated cells had subcortical actin aggregates. Thus, in contrast to TCDD, BaP produces a loss of cell attachment involving decreased localization of molecules important for cell-cell interactions in RL95-2 cells.

Original languageEnglish (US)
Pages (from-to)101-107
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume294
Issue number1
DOIs
StatePublished - 2002
Externally publishedYes

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Benzo(a)pyrene
Cell adhesion
Cell Adhesion
Vinculin
Catenins
Cadherins
Proteins
Actins
Cells
Phalloidine
Aryl Hydrocarbon Receptors
Cell membranes
Fluorescein
Epidermal Growth Factor Receptor
Adherens Junctions
Intercellular Junctions
1,4-dioxin
Polychlorinated Dibenzodioxins
Actin Cytoskeleton
Peptides

Keywords

  • β-catenin
  • Adhesion
  • Attachment
  • BaP
  • Cadherin
  • Cytoskeleton
  • EGF-R
  • Human uterine endometrium
  • TCDD

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Benzo(a)pyrene, but not 2,3,7,8-tetrachlorodibenzo-p-dioxin, alters cell adhesion proteins in human uterine RL95-2 cells. / McGarry, Michelle A.; Charles, Grantley D.; Medrano, Theresa; Bubb, Michael R.; Grant, Maria B.; Campbell-Thompson, Martha; Shiverick, Kathleen T.

In: Biochemical and Biophysical Research Communications, Vol. 294, No. 1, 2002, p. 101-107.

Research output: Contribution to journalArticle

McGarry, Michelle A. ; Charles, Grantley D. ; Medrano, Theresa ; Bubb, Michael R. ; Grant, Maria B. ; Campbell-Thompson, Martha ; Shiverick, Kathleen T. / Benzo(a)pyrene, but not 2,3,7,8-tetrachlorodibenzo-p-dioxin, alters cell adhesion proteins in human uterine RL95-2 cells. In: Biochemical and Biophysical Research Communications. 2002 ; Vol. 294, No. 1. pp. 101-107.
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