Bid activates multiple mitochondrial apoptotic mechanisms in primary hepatocytes after death receptor engagement

Yongge Zhao, Wen Xing Ding, Ting Qian, Simon Watkins, John J. Lemasters, Xiao-Ming Yin

Research output: Contribution to journalArticle

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Abstract

Background & Aims: Activation of Fas or tumor necrosis factor receptor 1 (TNF-R1) on hepatocytes leads to apoptosis, which requires mitochondria activation. The pro-death Bcl-2 family protein, Bid, mediates this pathway by inducing mitochondrial releases of cytochrome c and other apoptotic factors. How Bid activates mitochondria has been studied in vitro with isolated mitochondria. We intended to study the mechanisms in intact hepatocytes so that findings could be made in a proper cellular context and would be more physiologically relevant. Methods: Hepatocytes were isolated from wild-type and bid-deficient mice and treated with antiFas or TNF-α. Mechanisms of mitochondria activation were dissected with genetic, biochemical, and morphologic approaches. Results: bid-deficient hepatocytes were much more resistant to apoptosis. Bid was required for permeability transition and mitochondria depolarization in addition to the previously defined release of cytochrome c. Permeability transition inhibitors cyclosporin A and aristolochic acid could inhibit mitochondria activation effectively, but not as much as the deletion of the bid gene, and they could not inhibit Bak oligomerization. In addition, mitochondria depolarization also could be induced by caspases, whose activation was mainly dependent on Bid. Conclusions: Bid may activate mitochondria by 2 mechanisms, one is related to permeability transition and the other is related to Bak oligomerization. Bid can further affect mitochondria potentials by indirectly regulating caspase activity. This in vivo study provides novel findings not previously disclosed by in vitro studies, and indicates the importance of several mechanisms in contributing Bid-mediated mitochondria dysfunction that could be potential cellular targets of intervention.

Original languageEnglish (US)
Pages (from-to)854-867
Number of pages14
JournalGastroenterology
Volume125
Issue number3
DOIs
StatePublished - Sep 1 2003
Externally publishedYes

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Death Domain Receptors
Hepatocytes
Mitochondria
Permeability
Caspases
Cytochromes c
BH3 Interacting Domain Death Agonist Protein
Apoptosis
Tumor Necrosis Factor Receptors
Gene Deletion
Cyclosporine
Molecular Biology

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Bid activates multiple mitochondrial apoptotic mechanisms in primary hepatocytes after death receptor engagement. / Zhao, Yongge; Ding, Wen Xing; Qian, Ting; Watkins, Simon; Lemasters, John J.; Yin, Xiao-Ming.

In: Gastroenterology, Vol. 125, No. 3, 01.09.2003, p. 854-867.

Research output: Contribution to journalArticle

Zhao, Yongge ; Ding, Wen Xing ; Qian, Ting ; Watkins, Simon ; Lemasters, John J. ; Yin, Xiao-Ming. / Bid activates multiple mitochondrial apoptotic mechanisms in primary hepatocytes after death receptor engagement. In: Gastroenterology. 2003 ; Vol. 125, No. 3. pp. 854-867.
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