Bid is a positive regulator for donor-derived lymphoid cell regeneration in γ-irradiated recipients

Hongmei Shen, Hui Yu, Paulina H. Liang, Richard XuFeng, Yifang Song, Xiaoxia Hu, Xiaoyun Chen, Xiao-Ming Yin, Tao Cheng

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Objective: Hematopoietic regeneration is regulated by cell survival proteins, such as the Bcl-2 family. Bid, a BH3-only protein of the Bcl-2 family, has multiple cellular functions and is involved in a variety of physiological or pathological conditions. We attempted to define its role in hematopoietic cell repopulation under the stress condition of bone marrow transplantation. Materials and Methods: We performed conventional or competitive bone marrow transplantation with donor hematopoietic cells from Bid -/- or Bid +/+ mice. Flow cytometry was used for quantification of hematopoietic stem cells, hematopoietic progenitor cells, and differentiated cells in different lineages (T, B, and myeloid cells). Single cell culture and homing assays were performed to further evaluate hematopoietic stem cell functions. Hematopoietic progenitor cells were also measured by the colony-forming cell culture. Results: Contrary to the widely recognized role of Bid as a pro-apoptotic protein, the absence of Bid significantly reduced the reconstitution of donor hematopoietic cells in γ-irradiated recipients. Interestingly, however, numbers of hematopoietic stem cells and hematopoietic progenitor cells and their functions were not overtly altered. Instead, the regeneration of donor T and B cells was significantly impaired in the absence of Bid. Further analysis indicated an accumulation of the triple-negative T-cell population in the thymus, and pro-B cells in the bone marrow. Conclusions: Our current study demonstrates a positive impact of Bid on hematopoietic regeneration mainly due to its unique effects on donor lymphopoiesis in the transplant recipients.

Original languageEnglish (US)
JournalExperimental Hematology
Volume39
Issue number9
DOIs
StatePublished - Sep 2011
Externally publishedYes

Fingerprint

Hematopoietic Stem Cells
Regeneration
Lymphocytes
Tissue Donors
Bone Marrow Transplantation
T-Lymphocytes
B-Lymphocytes
Cell Culture Techniques
Lymphopoiesis
Apoptosis Regulatory Proteins
B-Lymphoid Precursor Cells
Myeloid Cells
Thymus Gland
Cell Survival
Flow Cytometry
Proteins
Bone Marrow
Population

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Hematology

Cite this

Bid is a positive regulator for donor-derived lymphoid cell regeneration in γ-irradiated recipients. / Shen, Hongmei; Yu, Hui; Liang, Paulina H.; XuFeng, Richard; Song, Yifang; Hu, Xiaoxia; Chen, Xiaoyun; Yin, Xiao-Ming; Cheng, Tao.

In: Experimental Hematology, Vol. 39, No. 9, 09.2011.

Research output: Contribution to journalArticle

Shen, Hongmei ; Yu, Hui ; Liang, Paulina H. ; XuFeng, Richard ; Song, Yifang ; Hu, Xiaoxia ; Chen, Xiaoyun ; Yin, Xiao-Ming ; Cheng, Tao. / Bid is a positive regulator for donor-derived lymphoid cell regeneration in γ-irradiated recipients. In: Experimental Hematology. 2011 ; Vol. 39, No. 9.
@article{b21d02a915af4356a6390cbd8bd65211,
title = "Bid is a positive regulator for donor-derived lymphoid cell regeneration in γ-irradiated recipients",
abstract = "Objective: Hematopoietic regeneration is regulated by cell survival proteins, such as the Bcl-2 family. Bid, a BH3-only protein of the Bcl-2 family, has multiple cellular functions and is involved in a variety of physiological or pathological conditions. We attempted to define its role in hematopoietic cell repopulation under the stress condition of bone marrow transplantation. Materials and Methods: We performed conventional or competitive bone marrow transplantation with donor hematopoietic cells from Bid -/- or Bid +/+ mice. Flow cytometry was used for quantification of hematopoietic stem cells, hematopoietic progenitor cells, and differentiated cells in different lineages (T, B, and myeloid cells). Single cell culture and homing assays were performed to further evaluate hematopoietic stem cell functions. Hematopoietic progenitor cells were also measured by the colony-forming cell culture. Results: Contrary to the widely recognized role of Bid as a pro-apoptotic protein, the absence of Bid significantly reduced the reconstitution of donor hematopoietic cells in γ-irradiated recipients. Interestingly, however, numbers of hematopoietic stem cells and hematopoietic progenitor cells and their functions were not overtly altered. Instead, the regeneration of donor T and B cells was significantly impaired in the absence of Bid. Further analysis indicated an accumulation of the triple-negative T-cell population in the thymus, and pro-B cells in the bone marrow. Conclusions: Our current study demonstrates a positive impact of Bid on hematopoietic regeneration mainly due to its unique effects on donor lymphopoiesis in the transplant recipients.",
author = "Hongmei Shen and Hui Yu and Liang, {Paulina H.} and Richard XuFeng and Yifang Song and Xiaoxia Hu and Xiaoyun Chen and Xiao-Ming Yin and Tao Cheng",
year = "2011",
month = "9",
doi = "10.1016/j.exphem.2011.06.004",
language = "English (US)",
volume = "39",
journal = "Experimental Hematology",
issn = "0301-472X",
publisher = "Elsevier Inc.",
number = "9",

}

TY - JOUR

T1 - Bid is a positive regulator for donor-derived lymphoid cell regeneration in γ-irradiated recipients

AU - Shen, Hongmei

AU - Yu, Hui

AU - Liang, Paulina H.

AU - XuFeng, Richard

AU - Song, Yifang

AU - Hu, Xiaoxia

AU - Chen, Xiaoyun

AU - Yin, Xiao-Ming

AU - Cheng, Tao

PY - 2011/9

Y1 - 2011/9

N2 - Objective: Hematopoietic regeneration is regulated by cell survival proteins, such as the Bcl-2 family. Bid, a BH3-only protein of the Bcl-2 family, has multiple cellular functions and is involved in a variety of physiological or pathological conditions. We attempted to define its role in hematopoietic cell repopulation under the stress condition of bone marrow transplantation. Materials and Methods: We performed conventional or competitive bone marrow transplantation with donor hematopoietic cells from Bid -/- or Bid +/+ mice. Flow cytometry was used for quantification of hematopoietic stem cells, hematopoietic progenitor cells, and differentiated cells in different lineages (T, B, and myeloid cells). Single cell culture and homing assays were performed to further evaluate hematopoietic stem cell functions. Hematopoietic progenitor cells were also measured by the colony-forming cell culture. Results: Contrary to the widely recognized role of Bid as a pro-apoptotic protein, the absence of Bid significantly reduced the reconstitution of donor hematopoietic cells in γ-irradiated recipients. Interestingly, however, numbers of hematopoietic stem cells and hematopoietic progenitor cells and their functions were not overtly altered. Instead, the regeneration of donor T and B cells was significantly impaired in the absence of Bid. Further analysis indicated an accumulation of the triple-negative T-cell population in the thymus, and pro-B cells in the bone marrow. Conclusions: Our current study demonstrates a positive impact of Bid on hematopoietic regeneration mainly due to its unique effects on donor lymphopoiesis in the transplant recipients.

AB - Objective: Hematopoietic regeneration is regulated by cell survival proteins, such as the Bcl-2 family. Bid, a BH3-only protein of the Bcl-2 family, has multiple cellular functions and is involved in a variety of physiological or pathological conditions. We attempted to define its role in hematopoietic cell repopulation under the stress condition of bone marrow transplantation. Materials and Methods: We performed conventional or competitive bone marrow transplantation with donor hematopoietic cells from Bid -/- or Bid +/+ mice. Flow cytometry was used for quantification of hematopoietic stem cells, hematopoietic progenitor cells, and differentiated cells in different lineages (T, B, and myeloid cells). Single cell culture and homing assays were performed to further evaluate hematopoietic stem cell functions. Hematopoietic progenitor cells were also measured by the colony-forming cell culture. Results: Contrary to the widely recognized role of Bid as a pro-apoptotic protein, the absence of Bid significantly reduced the reconstitution of donor hematopoietic cells in γ-irradiated recipients. Interestingly, however, numbers of hematopoietic stem cells and hematopoietic progenitor cells and their functions were not overtly altered. Instead, the regeneration of donor T and B cells was significantly impaired in the absence of Bid. Further analysis indicated an accumulation of the triple-negative T-cell population in the thymus, and pro-B cells in the bone marrow. Conclusions: Our current study demonstrates a positive impact of Bid on hematopoietic regeneration mainly due to its unique effects on donor lymphopoiesis in the transplant recipients.

UR - http://www.scopus.com/inward/record.url?scp=79961243711&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79961243711&partnerID=8YFLogxK

U2 - 10.1016/j.exphem.2011.06.004

DO - 10.1016/j.exphem.2011.06.004

M3 - Article

VL - 39

JO - Experimental Hematology

JF - Experimental Hematology

SN - 0301-472X

IS - 9

ER -