Biliary lipids and cholesterol crystal formation in leptin-deficient obese mice

Deborah A. Swartz-Basile, Matthew I. Goldblatt, Seong Ho Choi, Carol Svatek, Khoi Tran, Attila Nakeeb, Henry A. Pitt

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background. Obesity is often associated with increased biliary cholesterol secretion resulting in cholesterol gallstone formation. We have previously demonstrated that leptin-deficient C57Bl/6J Lep ob obese mice have abnormal biliary motility and are prone to cholesterol crystal formation. In addition, others have demonstrated that leptin-deficient mice when fed a lithogenic diet for eight weeks are not prone to gallstone formation. However, the biliary lipid and in vivo cholesterol crystal response of homozygous and heterozygous leptin-deficient mice to four weeks on a lithogenic diet has not been studied. Therefore, we tested the hypothesis that lithogenic diets influence gallbladder bile composition, serum lipids and cholesterol crystal formation in homozygous and heterozygous leptin-deficient mice compared to normal lean controls. Methods. 319 female lean control mice, 280 heterozygous lep ob obese mice and 117 homozygous lep ob obese mice were studied. Mice were fed either a lithogenic or control non-lithogenic chow diet for four weeks. Gallbladder volumes were measured, and bile was pooled to calculate cholesterol saturation indices. Serum cholesterol, glucose, and leptin levels were determined. Hepatic fat vacuoles were counted, and bile was observed microscopically for cholesterol crystal formation. Results. The lithogenic diet and mouse strain influenced body and liver weights, gallbladder volume, cholesterol crystal formation, serum cholesterol, glucose and leptin levels and hepatic fat vacuole numbers. However, only diet, not strain, altered biliary cholesterol saturation. Conclusion. The association among obesity, leptin, and gallstone formation may be primarily related to altered gallbladder motility and cholesterol crystal formation and only secondarily to biliary cholesterol saturation.

Original languageEnglish (US)
Pages (from-to)386-392
Number of pages7
JournalHPB
Volume8
Issue number5
DOIs
StatePublished - Oct 2006
Externally publishedYes

Fingerprint

Obese Mice
Leptin
Cholesterol
Lipids
Diet
Gallbladder
Gallstones
Bile
Vacuoles
Liver
Obesity
Serum
Fats
Glucose

Keywords

  • Bile
  • Cholesterol
  • Diabetes
  • Gallbladder
  • Obesity
  • Strain

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Swartz-Basile, D. A., Goldblatt, M. I., Ho Choi, S., Svatek, C., Tran, K., Nakeeb, A., & Pitt, H. A. (2006). Biliary lipids and cholesterol crystal formation in leptin-deficient obese mice. HPB, 8(5), 386-392. https://doi.org/10.1080/13651820600641233

Biliary lipids and cholesterol crystal formation in leptin-deficient obese mice. / Swartz-Basile, Deborah A.; Goldblatt, Matthew I.; Ho Choi, Seong; Svatek, Carol; Tran, Khoi; Nakeeb, Attila; Pitt, Henry A.

In: HPB, Vol. 8, No. 5, 10.2006, p. 386-392.

Research output: Contribution to journalArticle

Swartz-Basile, DA, Goldblatt, MI, Ho Choi, S, Svatek, C, Tran, K, Nakeeb, A & Pitt, HA 2006, 'Biliary lipids and cholesterol crystal formation in leptin-deficient obese mice', HPB, vol. 8, no. 5, pp. 386-392. https://doi.org/10.1080/13651820600641233
Swartz-Basile DA, Goldblatt MI, Ho Choi S, Svatek C, Tran K, Nakeeb A et al. Biliary lipids and cholesterol crystal formation in leptin-deficient obese mice. HPB. 2006 Oct;8(5):386-392. https://doi.org/10.1080/13651820600641233
Swartz-Basile, Deborah A. ; Goldblatt, Matthew I. ; Ho Choi, Seong ; Svatek, Carol ; Tran, Khoi ; Nakeeb, Attila ; Pitt, Henry A. / Biliary lipids and cholesterol crystal formation in leptin-deficient obese mice. In: HPB. 2006 ; Vol. 8, No. 5. pp. 386-392.
@article{4139bc01eb3b4094bddbda2521305e03,
title = "Biliary lipids and cholesterol crystal formation in leptin-deficient obese mice",
abstract = "Background. Obesity is often associated with increased biliary cholesterol secretion resulting in cholesterol gallstone formation. We have previously demonstrated that leptin-deficient C57Bl/6J Lep ob obese mice have abnormal biliary motility and are prone to cholesterol crystal formation. In addition, others have demonstrated that leptin-deficient mice when fed a lithogenic diet for eight weeks are not prone to gallstone formation. However, the biliary lipid and in vivo cholesterol crystal response of homozygous and heterozygous leptin-deficient mice to four weeks on a lithogenic diet has not been studied. Therefore, we tested the hypothesis that lithogenic diets influence gallbladder bile composition, serum lipids and cholesterol crystal formation in homozygous and heterozygous leptin-deficient mice compared to normal lean controls. Methods. 319 female lean control mice, 280 heterozygous lep ob obese mice and 117 homozygous lep ob obese mice were studied. Mice were fed either a lithogenic or control non-lithogenic chow diet for four weeks. Gallbladder volumes were measured, and bile was pooled to calculate cholesterol saturation indices. Serum cholesterol, glucose, and leptin levels were determined. Hepatic fat vacuoles were counted, and bile was observed microscopically for cholesterol crystal formation. Results. The lithogenic diet and mouse strain influenced body and liver weights, gallbladder volume, cholesterol crystal formation, serum cholesterol, glucose and leptin levels and hepatic fat vacuole numbers. However, only diet, not strain, altered biliary cholesterol saturation. Conclusion. The association among obesity, leptin, and gallstone formation may be primarily related to altered gallbladder motility and cholesterol crystal formation and only secondarily to biliary cholesterol saturation.",
keywords = "Bile, Cholesterol, Diabetes, Gallbladder, Obesity, Strain",
author = "Swartz-Basile, {Deborah A.} and Goldblatt, {Matthew I.} and {Ho Choi}, Seong and Carol Svatek and Khoi Tran and Attila Nakeeb and Pitt, {Henry A.}",
year = "2006",
month = "10",
doi = "10.1080/13651820600641233",
language = "English (US)",
volume = "8",
pages = "386--392",
journal = "HPB",
issn = "1365-182X",
publisher = "John Wiley and Sons Inc.",
number = "5",

}

TY - JOUR

T1 - Biliary lipids and cholesterol crystal formation in leptin-deficient obese mice

AU - Swartz-Basile, Deborah A.

AU - Goldblatt, Matthew I.

AU - Ho Choi, Seong

AU - Svatek, Carol

AU - Tran, Khoi

AU - Nakeeb, Attila

AU - Pitt, Henry A.

PY - 2006/10

Y1 - 2006/10

N2 - Background. Obesity is often associated with increased biliary cholesterol secretion resulting in cholesterol gallstone formation. We have previously demonstrated that leptin-deficient C57Bl/6J Lep ob obese mice have abnormal biliary motility and are prone to cholesterol crystal formation. In addition, others have demonstrated that leptin-deficient mice when fed a lithogenic diet for eight weeks are not prone to gallstone formation. However, the biliary lipid and in vivo cholesterol crystal response of homozygous and heterozygous leptin-deficient mice to four weeks on a lithogenic diet has not been studied. Therefore, we tested the hypothesis that lithogenic diets influence gallbladder bile composition, serum lipids and cholesterol crystal formation in homozygous and heterozygous leptin-deficient mice compared to normal lean controls. Methods. 319 female lean control mice, 280 heterozygous lep ob obese mice and 117 homozygous lep ob obese mice were studied. Mice were fed either a lithogenic or control non-lithogenic chow diet for four weeks. Gallbladder volumes were measured, and bile was pooled to calculate cholesterol saturation indices. Serum cholesterol, glucose, and leptin levels were determined. Hepatic fat vacuoles were counted, and bile was observed microscopically for cholesterol crystal formation. Results. The lithogenic diet and mouse strain influenced body and liver weights, gallbladder volume, cholesterol crystal formation, serum cholesterol, glucose and leptin levels and hepatic fat vacuole numbers. However, only diet, not strain, altered biliary cholesterol saturation. Conclusion. The association among obesity, leptin, and gallstone formation may be primarily related to altered gallbladder motility and cholesterol crystal formation and only secondarily to biliary cholesterol saturation.

AB - Background. Obesity is often associated with increased biliary cholesterol secretion resulting in cholesterol gallstone formation. We have previously demonstrated that leptin-deficient C57Bl/6J Lep ob obese mice have abnormal biliary motility and are prone to cholesterol crystal formation. In addition, others have demonstrated that leptin-deficient mice when fed a lithogenic diet for eight weeks are not prone to gallstone formation. However, the biliary lipid and in vivo cholesterol crystal response of homozygous and heterozygous leptin-deficient mice to four weeks on a lithogenic diet has not been studied. Therefore, we tested the hypothesis that lithogenic diets influence gallbladder bile composition, serum lipids and cholesterol crystal formation in homozygous and heterozygous leptin-deficient mice compared to normal lean controls. Methods. 319 female lean control mice, 280 heterozygous lep ob obese mice and 117 homozygous lep ob obese mice were studied. Mice were fed either a lithogenic or control non-lithogenic chow diet for four weeks. Gallbladder volumes were measured, and bile was pooled to calculate cholesterol saturation indices. Serum cholesterol, glucose, and leptin levels were determined. Hepatic fat vacuoles were counted, and bile was observed microscopically for cholesterol crystal formation. Results. The lithogenic diet and mouse strain influenced body and liver weights, gallbladder volume, cholesterol crystal formation, serum cholesterol, glucose and leptin levels and hepatic fat vacuole numbers. However, only diet, not strain, altered biliary cholesterol saturation. Conclusion. The association among obesity, leptin, and gallstone formation may be primarily related to altered gallbladder motility and cholesterol crystal formation and only secondarily to biliary cholesterol saturation.

KW - Bile

KW - Cholesterol

KW - Diabetes

KW - Gallbladder

KW - Obesity

KW - Strain

UR - http://www.scopus.com/inward/record.url?scp=33749160487&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33749160487&partnerID=8YFLogxK

U2 - 10.1080/13651820600641233

DO - 10.1080/13651820600641233

M3 - Article

C2 - 18333092

AN - SCOPUS:33749160487

VL - 8

SP - 386

EP - 392

JO - HPB

JF - HPB

SN - 1365-182X

IS - 5

ER -