Binary Fission of Pneumocystis carinii Trophozoites Grown in Vitro

J. D. RICHARDSON, S. F. QUEENER, M. BARTLETT, J. SMITH

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Trophozoites grown in vitro were shown to undergo binary fission by transmission electron microscopy (TEM). Standard fixation with subsequent embedding in Spurr was employed using 3% gluuraldehydc and 1% osmium tetroxide with 5% sucrose added to bom fixatives and 0.1 M cacodylate buffer washes. Trophozoites were grown on WI‐38 cells in vitro. Trophozoites were found in various stages of fission. The dividing trophozoite has daughter cells that arc rounder than the pleomorphic, non‐dividing trophozoites. Tubular forms external to the dividing trophozoites were decreased in number; tubular forms when present were concentrated around the forming septa. Nuclear material was sometimes, but not always, well defined in both daughter cells. There was no concentration of nuclear material at the poles. Vacuoles without membrane were present in the dividing forms. Separate nuclear regions were sometimes found in the dividing trophozoites. These observations suggest that binary fission does occur in culture; however, the significance of binary fission to the life cycle of Pneumocystis carinii (Pc) is not yet clear.

Original languageEnglish (US)
Pages (from-to)27S-29S
JournalThe Journal of Protozoology
Volume36
Issue number1
DOIs
StatePublished - Jan 1989

ASJC Scopus subject areas

  • Parasitology

Fingerprint Dive into the research topics of 'Binary Fission of Pneumocystis carinii Trophozoites Grown in Vitro'. Together they form a unique fingerprint.

  • Cite this