Binding of epidermal growth factor in rat pancreatic acini

Murray Korc, Lynn M. Matrisian, Stephen R. Planck, Bruce E. Magun

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Specific, saturable EGF receptors were demonstrated in isolated rat pancreatic acini. Binding of EGF to these receptors was one-half maximal at 20 min and maximal at 120 min. Scatchard analyses revealed a single order of binding sites with a Kd of 4.90 nM. Following binding, EGF was rapidly internalized and converted to two acidic species. EGF did not alter either basal amylase release or the rate of [3H]phenylalanine incorporation into TCA-precipitable protein. The finding of high affinity EGF receptors in pancreatic acinar cells supports the hypothesis that EGF participates in the long-term regulation of pancreatic exocrine function.

Original languageEnglish (US)
Pages (from-to)1066-1073
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume111
Issue number3
DOIs
StatePublished - Mar 29 1983
Externally publishedYes

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Epidermal Growth Factor
Rats
Acinar Cells
Amylases
Phenylalanine
Binding Sites
ErbB Receptors
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Binding of epidermal growth factor in rat pancreatic acini. / Korc, Murray; Matrisian, Lynn M.; Planck, Stephen R.; Magun, Bruce E.

In: Biochemical and Biophysical Research Communications, Vol. 111, No. 3, 29.03.1983, p. 1066-1073.

Research output: Contribution to journalArticle

Korc, Murray ; Matrisian, Lynn M. ; Planck, Stephen R. ; Magun, Bruce E. / Binding of epidermal growth factor in rat pancreatic acini. In: Biochemical and Biophysical Research Communications. 1983 ; Vol. 111, No. 3. pp. 1066-1073.
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