Binding of epidermal growth factor in rat pancreatic acini

Murray Korc, Lynn M. Matrisian, Stephen R. Planck, Bruce E. Magun

Research output: Contribution to journalArticle

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Abstract

Specific, saturable EGF receptors were demonstrated in isolated rat pancreatic acini. Binding of EGF to these receptors was one-half maximal at 20 min and maximal at 120 min. Scatchard analyses revealed a single order of binding sites with a Kd of 4.90 nM. Following binding, EGF was rapidly internalized and converted to two acidic species. EGF did not alter either basal amylase release or the rate of [3H]phenylalanine incorporation into TCA-precipitable protein. The finding of high affinity EGF receptors in pancreatic acinar cells supports the hypothesis that EGF participates in the long-term regulation of pancreatic exocrine function.

Original languageEnglish (US)
Pages (from-to)1066-1073
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume111
Issue number3
DOIs
StatePublished - Mar 29 1983

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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