Binding of medroxyprogesterone acetate in human breast cancer

P. C M Young, F. K. Keen, Lawrence Einhorn, B. M. Stanich, C. E. Ehrlich, R. E. Cleary

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Human mammary tumor cytosol contained macromolecules which bound 3H-MPA (3H-medroxyprogesterone acetate or 1,2-3H-6α-methyl-17α-acetoxy-pregn-4-ene-3,20-dione) and 3H-R5020 (6,7-3H-17,21-dimethyl-19-nor-pregna-4,9-diene-3,20-dione) similarly with high affinity (Ka ≈ 2 nM-1) and specificity. The progestin-binding components had sedimentation coefficients of about 4S and 7S in sucrose gradients and had approximately the same number of binding sites for MPA and R5020 as revealed by gradient centrifugation and saturation analysis. Among the steroids tested, these components had the highest affinities for progestins and were probably progesterone receptors of human breast cancer. A 4S component of human serum bound 3H-R5020 but not 3H-MPA. With 3H-MPA and 3H-estradiol used as the tracers, the concentrations of progesterone and estrogen receptors have been determined in 236 human breast cancers by saturation analysis. Our results on the receptor content and response of 31 of these tumors to endocrine therapy suggest that progesterone receptor may be a better marker of a hormonally responsive breast cancer.

Original languageEnglish
Pages (from-to)284-292
Number of pages9
JournalAmerican Journal of Obstetrics and Gynecology
Volume137
Issue number3
StatePublished - Jun 1 1980

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Medroxyprogesterone Acetate
Promegestone
Progesterone Receptors
Breast Neoplasms
Progestins
Centrifugation
Estrogen Receptors
Cytosol
Progesterone
Sucrose
Estradiol
Steroids
Binding Sites
Serum
Neoplasms
Therapeutics

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynecology

Cite this

Young, P. C. M., Keen, F. K., Einhorn, L., Stanich, B. M., Ehrlich, C. E., & Cleary, R. E. (1980). Binding of medroxyprogesterone acetate in human breast cancer. American Journal of Obstetrics and Gynecology, 137(3), 284-292.

Binding of medroxyprogesterone acetate in human breast cancer. / Young, P. C M; Keen, F. K.; Einhorn, Lawrence; Stanich, B. M.; Ehrlich, C. E.; Cleary, R. E.

In: American Journal of Obstetrics and Gynecology, Vol. 137, No. 3, 01.06.1980, p. 284-292.

Research output: Contribution to journalArticle

Young, PCM, Keen, FK, Einhorn, L, Stanich, BM, Ehrlich, CE & Cleary, RE 1980, 'Binding of medroxyprogesterone acetate in human breast cancer', American Journal of Obstetrics and Gynecology, vol. 137, no. 3, pp. 284-292.
Young PCM, Keen FK, Einhorn L, Stanich BM, Ehrlich CE, Cleary RE. Binding of medroxyprogesterone acetate in human breast cancer. American Journal of Obstetrics and Gynecology. 1980 Jun 1;137(3):284-292.
Young, P. C M ; Keen, F. K. ; Einhorn, Lawrence ; Stanich, B. M. ; Ehrlich, C. E. ; Cleary, R. E. / Binding of medroxyprogesterone acetate in human breast cancer. In: American Journal of Obstetrics and Gynecology. 1980 ; Vol. 137, No. 3. pp. 284-292.
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