Binding of tritiated dehydroretronecine to macromolecules

I. C. Hsu, Kent Robertson, R. C. Shumaker, J. R. Allen

Research output: Chapter in Book/Report/Conference proceedingChapter

21 Citations (Scopus)

Abstract

In vivo and in vitro experiments have shown that the pyrrolizidine alkaloid metabolite dehydroretronecine binds readily to macromolecules. In the in vivo experiment there was a preferential binding of dehydroretronecine to the gastric mucosa. Further extraction of the mucosa revealed a large percentage of the 3H was bound to the protein fraction and to a much lesser extent to DNA and RNA. The influence of pH on the binding of dehydroretronecine was substantiated in the in vitro experiment. Dehydroretronecine bound to calf thymus DNA and bovine serum albumin most readily under acidic conditions. These data suggest a direct correlation of the levels of dehydroretronecine binding to cellular macromolecules with the lesions that develop in affected organs.

Original languageEnglish (US)
Title of host publicationRES.COMMUN.CHEM.PATH.PHARMACOL.
Pages99-106
Number of pages8
Volume11
Edition1
StatePublished - 1975
Externally publishedYes

Fingerprint

Pyrrolizidine Alkaloids
Bovine Serum Albumin
Gastric Mucosa
Mucous Membrane
dehydroretronecine
RNA
DNA
Proteins
In Vitro Techniques
calf thymus DNA

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Hsu, I. C., Robertson, K., Shumaker, R. C., & Allen, J. R. (1975). Binding of tritiated dehydroretronecine to macromolecules. In RES.COMMUN.CHEM.PATH.PHARMACOL. (1 ed., Vol. 11, pp. 99-106)

Binding of tritiated dehydroretronecine to macromolecules. / Hsu, I. C.; Robertson, Kent; Shumaker, R. C.; Allen, J. R.

RES.COMMUN.CHEM.PATH.PHARMACOL.. Vol. 11 1. ed. 1975. p. 99-106.

Research output: Chapter in Book/Report/Conference proceedingChapter

Hsu, IC, Robertson, K, Shumaker, RC & Allen, JR 1975, Binding of tritiated dehydroretronecine to macromolecules. in RES.COMMUN.CHEM.PATH.PHARMACOL.. 1 edn, vol. 11, pp. 99-106.
Hsu IC, Robertson K, Shumaker RC, Allen JR. Binding of tritiated dehydroretronecine to macromolecules. In RES.COMMUN.CHEM.PATH.PHARMACOL.. 1 ed. Vol. 11. 1975. p. 99-106
Hsu, I. C. ; Robertson, Kent ; Shumaker, R. C. ; Allen, J. R. / Binding of tritiated dehydroretronecine to macromolecules. RES.COMMUN.CHEM.PATH.PHARMACOL.. Vol. 11 1. ed. 1975. pp. 99-106
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