Binding sites for peptidoglycan on mouse lymphocytes

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Binding of peptidoglycan (PG), a B-cell mitogen and polyclonal activator, to mouse lymphocytes was studied using resetting with PG-sensitized erythrocytes and a direct binding assay with 125I-labeled PG. Thirty-four percent of splenic lymphocytes formed PG rosettes, 62% of which were inhibited by preincubation of lymphocytes with free PG. Less than 1 or 3% of spleen cells formed rosettes with uncoated or albumin-coated red cells. The formation of rosettes was not inhibited by 0.1% azide and was not dependent on the presence of complement or immunoglobulins. The 125I-PG bound both specifically and nonspecifically to the lymphocytes. The binding was completed within 15-20 min, was proportional to the cell concentration, and was not inhibited by 0.1% azide or treatment of lymphocytes with formalin. The cells had one set of specific binding sites of low affinity (KD = 1.2-4.6 × 10-7 M ± 9% SE, based on competition experiments). The binding, however, was complex, probably involving interaction of multiple binding sites on PG with the cell surface. The EC50 (920 μg/ml) was similar to the optimal lymphocyte-activating concentration of PG (400-1000 μg/ml). The binding correlated with the ability of different PG preparations to stimulate lymphocytes, since only high Mr, PG (not low Mr PG preparations, muramyl dipeptide (MDP), or PG pentapeptide) had the ability to specifically bind to lymphocytes, to compete with PG binding, and to stimulate lymphocytes. Also, low Mr PG preparations, MDP, or PG pentapeptide did not inhibit the mitogenic stimulation of lymphocytes by high Mr PG. These results indicate the presence of specific binding sites for PG on the surface of murine lymphocytes and suggest that the binding of PG to these binding sites is involved in lymphocyte activation by PG.

Original languageEnglish
Pages (from-to)231-245
Number of pages15
JournalCellular Immunology
Volume109
Issue number1
DOIs
StatePublished - Oct 1 1987

Fingerprint

Peptidoglycan
Binding Sites
Lymphocytes
Acetylmuramyl-Alanyl-Isoglutamine
Azides
Lymphocyte Activation
Rosette Formation

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Binding sites for peptidoglycan on mouse lymphocytes. / Dziarski, Roman.

In: Cellular Immunology, Vol. 109, No. 1, 01.10.1987, p. 231-245.

Research output: Contribution to journalArticle

@article{2c78baaedbf04201aa8a25add8e7b14b,
title = "Binding sites for peptidoglycan on mouse lymphocytes",
abstract = "Binding of peptidoglycan (PG), a B-cell mitogen and polyclonal activator, to mouse lymphocytes was studied using resetting with PG-sensitized erythrocytes and a direct binding assay with 125I-labeled PG. Thirty-four percent of splenic lymphocytes formed PG rosettes, 62{\%} of which were inhibited by preincubation of lymphocytes with free PG. Less than 1 or 3{\%} of spleen cells formed rosettes with uncoated or albumin-coated red cells. The formation of rosettes was not inhibited by 0.1{\%} azide and was not dependent on the presence of complement or immunoglobulins. The 125I-PG bound both specifically and nonspecifically to the lymphocytes. The binding was completed within 15-20 min, was proportional to the cell concentration, and was not inhibited by 0.1{\%} azide or treatment of lymphocytes with formalin. The cells had one set of specific binding sites of low affinity (KD = 1.2-4.6 × 10-7 M ± 9{\%} SE, based on competition experiments). The binding, however, was complex, probably involving interaction of multiple binding sites on PG with the cell surface. The EC50 (920 μg/ml) was similar to the optimal lymphocyte-activating concentration of PG (400-1000 μg/ml). The binding correlated with the ability of different PG preparations to stimulate lymphocytes, since only high Mr, PG (not low Mr PG preparations, muramyl dipeptide (MDP), or PG pentapeptide) had the ability to specifically bind to lymphocytes, to compete with PG binding, and to stimulate lymphocytes. Also, low Mr PG preparations, MDP, or PG pentapeptide did not inhibit the mitogenic stimulation of lymphocytes by high Mr PG. These results indicate the presence of specific binding sites for PG on the surface of murine lymphocytes and suggest that the binding of PG to these binding sites is involved in lymphocyte activation by PG.",
author = "Roman Dziarski",
year = "1987",
month = "10",
day = "1",
doi = "10.1016/0008-8749(87)90307-8",
language = "English",
volume = "109",
pages = "231--245",
journal = "Cellular Immunology",
issn = "0008-8749",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Binding sites for peptidoglycan on mouse lymphocytes

AU - Dziarski, Roman

PY - 1987/10/1

Y1 - 1987/10/1

N2 - Binding of peptidoglycan (PG), a B-cell mitogen and polyclonal activator, to mouse lymphocytes was studied using resetting with PG-sensitized erythrocytes and a direct binding assay with 125I-labeled PG. Thirty-four percent of splenic lymphocytes formed PG rosettes, 62% of which were inhibited by preincubation of lymphocytes with free PG. Less than 1 or 3% of spleen cells formed rosettes with uncoated or albumin-coated red cells. The formation of rosettes was not inhibited by 0.1% azide and was not dependent on the presence of complement or immunoglobulins. The 125I-PG bound both specifically and nonspecifically to the lymphocytes. The binding was completed within 15-20 min, was proportional to the cell concentration, and was not inhibited by 0.1% azide or treatment of lymphocytes with formalin. The cells had one set of specific binding sites of low affinity (KD = 1.2-4.6 × 10-7 M ± 9% SE, based on competition experiments). The binding, however, was complex, probably involving interaction of multiple binding sites on PG with the cell surface. The EC50 (920 μg/ml) was similar to the optimal lymphocyte-activating concentration of PG (400-1000 μg/ml). The binding correlated with the ability of different PG preparations to stimulate lymphocytes, since only high Mr, PG (not low Mr PG preparations, muramyl dipeptide (MDP), or PG pentapeptide) had the ability to specifically bind to lymphocytes, to compete with PG binding, and to stimulate lymphocytes. Also, low Mr PG preparations, MDP, or PG pentapeptide did not inhibit the mitogenic stimulation of lymphocytes by high Mr PG. These results indicate the presence of specific binding sites for PG on the surface of murine lymphocytes and suggest that the binding of PG to these binding sites is involved in lymphocyte activation by PG.

AB - Binding of peptidoglycan (PG), a B-cell mitogen and polyclonal activator, to mouse lymphocytes was studied using resetting with PG-sensitized erythrocytes and a direct binding assay with 125I-labeled PG. Thirty-four percent of splenic lymphocytes formed PG rosettes, 62% of which were inhibited by preincubation of lymphocytes with free PG. Less than 1 or 3% of spleen cells formed rosettes with uncoated or albumin-coated red cells. The formation of rosettes was not inhibited by 0.1% azide and was not dependent on the presence of complement or immunoglobulins. The 125I-PG bound both specifically and nonspecifically to the lymphocytes. The binding was completed within 15-20 min, was proportional to the cell concentration, and was not inhibited by 0.1% azide or treatment of lymphocytes with formalin. The cells had one set of specific binding sites of low affinity (KD = 1.2-4.6 × 10-7 M ± 9% SE, based on competition experiments). The binding, however, was complex, probably involving interaction of multiple binding sites on PG with the cell surface. The EC50 (920 μg/ml) was similar to the optimal lymphocyte-activating concentration of PG (400-1000 μg/ml). The binding correlated with the ability of different PG preparations to stimulate lymphocytes, since only high Mr, PG (not low Mr PG preparations, muramyl dipeptide (MDP), or PG pentapeptide) had the ability to specifically bind to lymphocytes, to compete with PG binding, and to stimulate lymphocytes. Also, low Mr PG preparations, MDP, or PG pentapeptide did not inhibit the mitogenic stimulation of lymphocytes by high Mr PG. These results indicate the presence of specific binding sites for PG on the surface of murine lymphocytes and suggest that the binding of PG to these binding sites is involved in lymphocyte activation by PG.

UR - http://www.scopus.com/inward/record.url?scp=0023205022&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023205022&partnerID=8YFLogxK

U2 - 10.1016/0008-8749(87)90307-8

DO - 10.1016/0008-8749(87)90307-8

M3 - Article

C2 - 2820589

AN - SCOPUS:0023205022

VL - 109

SP - 231

EP - 245

JO - Cellular Immunology

JF - Cellular Immunology

SN - 0008-8749

IS - 1

ER -