Bioactive Lipids in Cancer, Inflammation and Related Diseases: Acute and Chronic Mild Traumatic Brain Injury Differentially Changes Levels of Bioactive Lipids in the CNS Associated with Headache

Emma Leishman, Phillip E. Kunkler, Joyce H. Hurley, Sally Miller, Heather B. Bradshaw

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Headache is a common complaint after mild traumatic brain injury (mTBI). Changes in the CNS lipidome were previously associated with acrolein-induced headache in rodents. mTBI caused similar headache-like symptoms in rats; therefore, we tested the hypothesis that mTBI might likewise alter the lipidome. Using a stereotaxic impactor, rats were given either a single mTBI or a series of 4 mTBIs 48 h apart. 72 h later for single mTBI and 7 days later for repeated mTBI, the trigeminal ganglia (TG), trigeminal nucleus (TNC), and cerebellum (CER) were isolated. Using HPLC/MS/MS, ~80 lipids were measured in each tissue and compared to sham controls. mTBI drove widespread alterations in lipid levels. Single mTBI increased arachidonic acid and repeated mTBI increased prostaglandins in all 3 tissue types. mTBI affected multiple TRPV agonists, including N-arachidonoyl ethanolamine (AEA), which increased in the TNC and CER after single mTBI. After repeated mTBI, AEA increased in the TG, but decreased in the TNC. Common to all tissue types in single and repeated mTBI was an increase the AEA metabolite, N-arachidonoyl glycine, a potent activator of microglial migration. Changes in the CNS lipidome associated with mTBI likely play a role in headache and in long-term neurodegenerative effects of repeated mTBI.

Original languageEnglish (US)
Title of host publicationAdvances in Experimental Medicine and Biology
PublisherSpringer New York LLC
Pages193-217
Number of pages25
DOIs
StatePublished - Jan 1 2019

Publication series

NameAdvances in Experimental Medicine and Biology
Volume1161
ISSN (Print)0065-2598
ISSN (Electronic)2214-8019

Keywords

  • Acyl amide
  • Acyl amino acid
  • BV-2
  • Docosahexaenoyl ethanolamine
  • Endocannabinoids
  • Lipidomics
  • Lipoamine
  • mTBI
  • N-arachidonoyl glycine
  • TRP

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Leishman, E., Kunkler, P. E., Hurley, J. H., Miller, S., & Bradshaw, H. B. (2019). Bioactive Lipids in Cancer, Inflammation and Related Diseases: Acute and Chronic Mild Traumatic Brain Injury Differentially Changes Levels of Bioactive Lipids in the CNS Associated with Headache. In Advances in Experimental Medicine and Biology (pp. 193-217). (Advances in Experimental Medicine and Biology; Vol. 1161). Springer New York LLC. https://doi.org/10.1007/978-3-030-21735-8_16