Bioactivity of novel self-assembled crystalline Nb2O5 microstructures in simulated and human salivas

Robert L. Karlinsey, Anderson Hara, Keewook Yi, Clif W. Duhn

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Crystalline, self-assembled niobium oxide microstructures formed in situ via potentiostatic anodization of niobium foil in an HF(aq) electrolyte solution are proposed as exceptional nucleators of Ca-P minerals, including hydroxyapatite. This material was tested for bioactivity through immersion in simulated and pooled human salivas. The simulated saliva formulation was based on mineral content found in stimulated human saliva and has a molar Ca/P ratio of 1:3.7. Oxide microstructures and mineral morphologies were examined using scanning electron microscopy. Differences in the mineral phase and morphology were attributed to the contrasting complexities of the two supersaturated solutions, with proteins and enzymes in human saliva most likely imparting a significant role. Dimensions of the niobium oxide microstructures and mineral deposits were characterized using profilometry. Energy dispersive spectroscopy, x-ray diffraction, Raman spectroscopy and electron microprobe analysis were utilized in identifying the nucleated mineral phases. Nucleation from human saliva resulted in mixed-phase mineral formations including amorphous calcium phosphate and poorly crystalline apatites. On the other hand, mineral nucleation from simulated saliva was more specific to hydroxyapatite. Based on these results, we demonstrate that a crystalline, self-assembled metal oxide is a unique and efficient nucleator of hydroxyapatite and other Ca-P minerals in supersaturated salivary solutions.

Original languageEnglish (US)
Article number003
Pages (from-to)16-23
Number of pages8
JournalBiomedical Materials (Bristol)
Volume1
Issue number1
DOIs
StatePublished - Mar 1 2006

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Bioactivity
Saliva
Minerals
Crystalline materials
Microstructure
Niobium
Oxides
Durapatite
Hydroxyapatite
Niobium oxide
Nucleation
Apatites
Profilometry
Mineral resources
Apatite
Calcium phosphate
Electron probe microanalysis
Raman Spectrum Analysis
Immersion
Metal foil

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Biophysics
  • Biomaterials

Cite this

Bioactivity of novel self-assembled crystalline Nb2O5 microstructures in simulated and human salivas. / Karlinsey, Robert L.; Hara, Anderson; Yi, Keewook; Duhn, Clif W.

In: Biomedical Materials (Bristol), Vol. 1, No. 1, 003, 01.03.2006, p. 16-23.

Research output: Contribution to journalArticle

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