Biochemical and genetic characterization of the porcine Prophet of Pit-1 pituitary transcription factor

Kyle W. Sloop, Amy McCutchan Schiller, Timothy P L Smith, John R. Blanton, Gary A. Rohrer, Bradley C. Meier, Simon Rhodes

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Prophet of Pit-1 (Prop-1) is a paired class homeodomain transcription factor that is specifically expressed in the pituitary gland. Mutations in the Prop-1 gene cause compound pituitary diseases in mouse models and human patients. We have cloned and analyzed the porcine ortholog of Prop-1. Analysis of cDNAs revealed that the porcine Prop-1 sequence is similar to the mouse and human proteins within the homeodomain and carboxyl terminus, but the amino terminus is poorly conserved. The Prop-1 gene consists of three exons and two introns and spans 3.8 kilobases of genomic DNA. In addition, we mapped Prop-1 to the q arm of pig chromosome two. During development, Prop-1 is expressed at the time of pituitary organogenesis. In the adult, expression was observed at low levels only in the pituitary gland. The porcine Prop-1 protein displays similar biochemical, DNA binding, and transcriptional activities to human PROP-1. We conclude that, although the structural divergence between the porcine and human PROP-1 molecules may indicate some distinct functions, the porcine Prop-1 gene encodes a pituitary transcription factor with similar overall activities to the human ortholog. (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)77-87
Number of pages11
JournalMolecular and Cellular Endocrinology
Volume168
Issue number1-2
DOIs
StatePublished - Oct 25 2000

Fingerprint

Transcription Factor Pit-1
Molecular Biology
Transcription Factors
Swine
Genes
DNA
Pituitary Gland
Chromosomes
Human Activities
Introns
Exons
Complementary DNA
Pituitary Diseases
Homeodomain Proteins
Molecules
Organogenesis
Proteins
Mutation

Keywords

  • Growth
  • Homeodomain
  • Mapping
  • Paired
  • Trophic hormone

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Biochemical and genetic characterization of the porcine Prophet of Pit-1 pituitary transcription factor. / Sloop, Kyle W.; McCutchan Schiller, Amy; Smith, Timothy P L; Blanton, John R.; Rohrer, Gary A.; Meier, Bradley C.; Rhodes, Simon.

In: Molecular and Cellular Endocrinology, Vol. 168, No. 1-2, 25.10.2000, p. 77-87.

Research output: Contribution to journalArticle

Sloop, Kyle W. ; McCutchan Schiller, Amy ; Smith, Timothy P L ; Blanton, John R. ; Rohrer, Gary A. ; Meier, Bradley C. ; Rhodes, Simon. / Biochemical and genetic characterization of the porcine Prophet of Pit-1 pituitary transcription factor. In: Molecular and Cellular Endocrinology. 2000 ; Vol. 168, No. 1-2. pp. 77-87.
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