Biodegradable alginate microspheres as a delivery system for naked DNA

Neeraj Aggarwal, Harm HogenEsch, Peixuan Guo, Adam North, Mark Suckow, Suresh K. Mittal

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

Sodium alginate is a naturally occurring polysaccharide that can easily be polymerized into a solid matrix to form microspheres. These biodegradable microspheres were used to encapsulate plasmid DNA containing the bacterial ß-galactosidase (LacZ) gene under the control of either the cytomegalovirus (CMV) immediate-early promoter or the Rous sarcoma virus (RSV) early promoter. Mice inoculated orally with microspheres containing plasmid DNA expressed LacZ in the intestine, spleen and liver. Inoculation of mice with microspheres containing both the plasmid DNA and bovine adenovirus type 3 (BAd3) resulted in a significant increase in LacZ expression compared to those inoculated with microspheres containing only the plasmid DNA. Our results suggest that adenoviruses are capable of augumenting transgene expression by plasmid DNA both in vitro and in vivo.

Original languageEnglish (US)
Pages (from-to)153-156
Number of pages4
JournalCanadian Journal of Veterinary Research
Volume63
Issue number2
StatePublished - Apr 1999

ASJC Scopus subject areas

  • veterinary(all)

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