Biodegradable alginate microspheres as a delivery system for naked DNA

Neeraj Aggarwal, Harm HogenEsch, Peixuan Guo, Adam North, Mark Suckow, Suresh K. Mittal

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Sodium alginate is a naturally occurring polysaccharide that can easily be polymerized into a solid matrix to form microspheres. These biodegradable microspheres were used to encapsulate plasmid DNA containing the bacterial ß-galactosidase (LacZ) gene under the control of either the cytomegalovirus (CMV) immediate-early promoter or the Rous sarcoma virus (RSV) early promoter. Mice inoculated orally with microspheres containing plasmid DNA expressed LacZ in the intestine, spleen and liver. Inoculation of mice with microspheres containing both the plasmid DNA and bovine adenovirus type 3 (BAd3) resulted in a significant increase in LacZ expression compared to those inoculated with microspheres containing only the plasmid DNA. Our results suggest that adenoviruses are capable of augumenting transgene expression by plasmid DNA both in vitro and in vivo.

Original languageEnglish (US)
Pages (from-to)153-156
Number of pages4
JournalCanadian Journal of Veterinary Research
Volume63
Issue number2
StatePublished - Apr 1999
Externally publishedYes

Fingerprint

biodegradability
alginates
Microspheres
plasmids
Plasmids
DNA
Adenoviridae
promoter regions
Galactosidases
Rous sarcoma virus
galactosidases
Cytomegalovirus
Bacterial DNA
Lac Operon
sodium alginate
mice
Transgenes
transgenes
Intestines
Polysaccharides

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Aggarwal, N., HogenEsch, H., Guo, P., North, A., Suckow, M., & Mittal, S. K. (1999). Biodegradable alginate microspheres as a delivery system for naked DNA. Canadian Journal of Veterinary Research, 63(2), 153-156.

Biodegradable alginate microspheres as a delivery system for naked DNA. / Aggarwal, Neeraj; HogenEsch, Harm; Guo, Peixuan; North, Adam; Suckow, Mark; Mittal, Suresh K.

In: Canadian Journal of Veterinary Research, Vol. 63, No. 2, 04.1999, p. 153-156.

Research output: Contribution to journalArticle

Aggarwal, N, HogenEsch, H, Guo, P, North, A, Suckow, M & Mittal, SK 1999, 'Biodegradable alginate microspheres as a delivery system for naked DNA', Canadian Journal of Veterinary Research, vol. 63, no. 2, pp. 153-156.
Aggarwal, Neeraj ; HogenEsch, Harm ; Guo, Peixuan ; North, Adam ; Suckow, Mark ; Mittal, Suresh K. / Biodegradable alginate microspheres as a delivery system for naked DNA. In: Canadian Journal of Veterinary Research. 1999 ; Vol. 63, No. 2. pp. 153-156.
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