Biodegradable nanofibrous drug delivery systems

effects of metronidazole and ciprofloxacin on periodontopathogens and commensal oral bacteria

Marco C. Bottino, Rodrigo A. Arthur, R. Aaron Waeiss, Krzysztof Kamocki, Karen S. Gregson, Richard Gregory

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Objectives: The purposes of this study were to fabricate biodegradable polydioxanone (PDS II®) electrospun periodontal drug delivery systems (hereafter referred to as matrices) containing either metronidazole (MET) or ciprofloxacin (CIP) and to investigate the effects of antibiotic incorporation on both periodontopathogens and commensal oral bacteria.

Materials and methods: Fibrous matrices were processed from PDS polymer solution by electrospinning. Antibiotic-containing PDS solutions were prepared to obtain four distinct groups: 5 wt.% MET, 25 wt.% MET, 5 wt.% CIP, and 25 wt.% CIP. Pure PDS was used as a control. High-performance liquid chromatography (HPLC) was done to evaluate MET and CIP release. Dual-species biofilms formed by Lactobacillus casei (Lc) and Streptococcus salivarius (Ss) were grown on the surface of all electrospun matrices. After 4 days of biofilm growth, the viability of bacteria on biofilms was assessed. Additionally, antimicrobial properties were evaluated against periodontopathogens Fusobacterium nucleatum (Fn) and Aggregatibacter actinomycetemcomitans (Aa) using agar diffusion assay.

Results: A three-dimensional interconnected porous network was observed in the different fabricated matrices. Pure PDS showed the highest fiber diameter mean (1,158 ± 402 nm) followed in a descending order by groups 5 wt.% MET (1,108 ± 383 nm), 25 wt.% MET (944 ± 392 nm), 5 wt.% CIP (871 ± 309 nm), and 25 wt.% CIP (765 ± 288 nm). HPLC demonstrated that groups containing higher amounts (25 wt.%) of incorporated drugs released more over time, while those with lower levels (5 wt.%) the least. No inhibitory effect of the tested antibiotics was detected on biofilm formation by the tested commensal oral bacteria. Meanwhile, CIP-containing matrices inhibited growth of Fn and Aa.

Conclusion: CIP-containing matrices led to a significant inhibition of periodontopathogens without negatively impairing the growth of periodontal beneficial bacteria.

Clinical relevance: Based on the proven in vitro inhibition of periodontitis-related bacteria, future in vivo research using relevant animal models is needed to confirm the effectiveness of these drug delivery systems.

Original languageEnglish
Pages (from-to)2151-2158
Number of pages8
JournalClinical Oral Investigations
Volume18
Issue number9
DOIs
StatePublished - Nov 16 2014

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Metronidazole
Drug Delivery Systems
Ciprofloxacin
Bacteria
Biofilms
Fusobacterium nucleatum
Aggregatibacter actinomycetemcomitans
Anti-Bacterial Agents
Growth
Polydioxanone
High Pressure Liquid Chromatography
Microbial Viability
Lactobacillus casei
Periodontitis
Agar
Polymers
Animal Models
Research
Pharmaceutical Preparations

Keywords

  • Antibiotics
  • Bacteria
  • Biofilm
  • Drug delivery
  • Electrospinning
  • Periodontitis

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Biodegradable nanofibrous drug delivery systems : effects of metronidazole and ciprofloxacin on periodontopathogens and commensal oral bacteria. / Bottino, Marco C.; Arthur, Rodrigo A.; Waeiss, R. Aaron; Kamocki, Krzysztof; Gregson, Karen S.; Gregory, Richard.

In: Clinical Oral Investigations, Vol. 18, No. 9, 16.11.2014, p. 2151-2158.

Research output: Contribution to journalArticle

Bottino, Marco C. ; Arthur, Rodrigo A. ; Waeiss, R. Aaron ; Kamocki, Krzysztof ; Gregson, Karen S. ; Gregory, Richard. / Biodegradable nanofibrous drug delivery systems : effects of metronidazole and ciprofloxacin on periodontopathogens and commensal oral bacteria. In: Clinical Oral Investigations. 2014 ; Vol. 18, No. 9. pp. 2151-2158.
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AU - Gregson, Karen S.

AU - Gregory, Richard

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N2 - Objectives: The purposes of this study were to fabricate biodegradable polydioxanone (PDS II®) electrospun periodontal drug delivery systems (hereafter referred to as matrices) containing either metronidazole (MET) or ciprofloxacin (CIP) and to investigate the effects of antibiotic incorporation on both periodontopathogens and commensal oral bacteria.Materials and methods: Fibrous matrices were processed from PDS polymer solution by electrospinning. Antibiotic-containing PDS solutions were prepared to obtain four distinct groups: 5 wt.% MET, 25 wt.% MET, 5 wt.% CIP, and 25 wt.% CIP. Pure PDS was used as a control. High-performance liquid chromatography (HPLC) was done to evaluate MET and CIP release. Dual-species biofilms formed by Lactobacillus casei (Lc) and Streptococcus salivarius (Ss) were grown on the surface of all electrospun matrices. After 4 days of biofilm growth, the viability of bacteria on biofilms was assessed. Additionally, antimicrobial properties were evaluated against periodontopathogens Fusobacterium nucleatum (Fn) and Aggregatibacter actinomycetemcomitans (Aa) using agar diffusion assay.Results: A three-dimensional interconnected porous network was observed in the different fabricated matrices. Pure PDS showed the highest fiber diameter mean (1,158 ± 402 nm) followed in a descending order by groups 5 wt.% MET (1,108 ± 383 nm), 25 wt.% MET (944 ± 392 nm), 5 wt.% CIP (871 ± 309 nm), and 25 wt.% CIP (765 ± 288 nm). HPLC demonstrated that groups containing higher amounts (25 wt.%) of incorporated drugs released more over time, while those with lower levels (5 wt.%) the least. No inhibitory effect of the tested antibiotics was detected on biofilm formation by the tested commensal oral bacteria. Meanwhile, CIP-containing matrices inhibited growth of Fn and Aa.Conclusion: CIP-containing matrices led to a significant inhibition of periodontopathogens without negatively impairing the growth of periodontal beneficial bacteria.Clinical relevance: Based on the proven in vitro inhibition of periodontitis-related bacteria, future in vivo research using relevant animal models is needed to confirm the effectiveness of these drug delivery systems.

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