Biodynamic imaging for phenotypic profiling of three-dimensional tissue culture

Hao Sun, Daniel Merrill, Ran An, John Turek, Daniela Matei, David D. Nolte

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Three-dimensional (3-D) tissue culture represents a more biologically relevant environment for testing new drugs compared to conventional two-dimensional cancer cell culture models. Biodynamic imaging is a high-content 3-D optical imaging technology based on low-coherence interferometry and digital holography that uses dynamic speckle as high-content image contrast to probe deep inside 3-D tissue. Speckle contrast is shown to be a scaling function of the acquisition time relative to the persistence time of intracellular transport and hence provides a measure of cellular activity. Cellular responses of 3-D multicellular spheroids to paclitaxel are compared among three different growth techniques: rotating bioreactor (BR), hanging-drop (HD), and nonadherent (U-bottom, UB) plate spheroids, compared with ex vivo living tissues. HD spheroids have the most homogeneous tissue, whereas BR spheroids display large sample-to-sample variability as well as spatial heterogeneity. The responses of BR-grown tumor spheroids to paclitaxel are more similar to those of ex vivo biopsies than the responses of spheroids grown using HD or plate methods. The rate of mitosis inhibition by application of taxol is measured through tissue dynamics spectroscopic imaging, demonstrating the ability to monitor antimitotic chemotherapy. These results illustrate the potential use of low-coherence digital holography for 3-D pharmaceutical screening applications.

Original languageEnglish (US)
Article number016007
JournalJournal of Biomedical Optics
Volume22
Issue number1
DOIs
StatePublished - Jan 1 2017
Externally publishedYes

Fingerprint

biodynamics
Tissue culture
spheroids
Paclitaxel
Bioreactors
Tissue
Imaging techniques
bioreactors
Holography
Speckle
Antimitotic Agents
holography
Chemotherapy
Biopsy
Cell culture
Interferometry
Pharmaceutical Preparations
Drug products
mitosis
Tumors

Keywords

  • biodynamic imaging
  • biological relevance
  • biomedical imaging
  • cellular dynamics
  • digital holography
  • drug development
  • drug discovery
  • dynamic light scattering
  • high-content imaging
  • low-coherence interferometry
  • pharmaceutical
  • physiological effects of paclitaxel
  • speckle
  • Three-dimensional tissue growth

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Biomedical Engineering

Cite this

Biodynamic imaging for phenotypic profiling of three-dimensional tissue culture. / Sun, Hao; Merrill, Daniel; An, Ran; Turek, John; Matei, Daniela; Nolte, David D.

In: Journal of Biomedical Optics, Vol. 22, No. 1, 016007, 01.01.2017.

Research output: Contribution to journalArticle

Sun, Hao ; Merrill, Daniel ; An, Ran ; Turek, John ; Matei, Daniela ; Nolte, David D. / Biodynamic imaging for phenotypic profiling of three-dimensional tissue culture. In: Journal of Biomedical Optics. 2017 ; Vol. 22, No. 1.
@article{444f5d1f9034400ba54216aa47889ee3,
title = "Biodynamic imaging for phenotypic profiling of three-dimensional tissue culture",
abstract = "Three-dimensional (3-D) tissue culture represents a more biologically relevant environment for testing new drugs compared to conventional two-dimensional cancer cell culture models. Biodynamic imaging is a high-content 3-D optical imaging technology based on low-coherence interferometry and digital holography that uses dynamic speckle as high-content image contrast to probe deep inside 3-D tissue. Speckle contrast is shown to be a scaling function of the acquisition time relative to the persistence time of intracellular transport and hence provides a measure of cellular activity. Cellular responses of 3-D multicellular spheroids to paclitaxel are compared among three different growth techniques: rotating bioreactor (BR), hanging-drop (HD), and nonadherent (U-bottom, UB) plate spheroids, compared with ex vivo living tissues. HD spheroids have the most homogeneous tissue, whereas BR spheroids display large sample-to-sample variability as well as spatial heterogeneity. The responses of BR-grown tumor spheroids to paclitaxel are more similar to those of ex vivo biopsies than the responses of spheroids grown using HD or plate methods. The rate of mitosis inhibition by application of taxol is measured through tissue dynamics spectroscopic imaging, demonstrating the ability to monitor antimitotic chemotherapy. These results illustrate the potential use of low-coherence digital holography for 3-D pharmaceutical screening applications.",
keywords = "biodynamic imaging, biological relevance, biomedical imaging, cellular dynamics, digital holography, drug development, drug discovery, dynamic light scattering, high-content imaging, low-coherence interferometry, pharmaceutical, physiological effects of paclitaxel, speckle, Three-dimensional tissue growth",
author = "Hao Sun and Daniel Merrill and Ran An and John Turek and Daniela Matei and Nolte, {David D.}",
year = "2017",
month = "1",
day = "1",
doi = "10.1117/1.JBO.22.1.016007",
language = "English (US)",
volume = "22",
journal = "Journal of Biomedical Optics",
issn = "1083-3668",
publisher = "SPIE",
number = "1",

}

TY - JOUR

T1 - Biodynamic imaging for phenotypic profiling of three-dimensional tissue culture

AU - Sun, Hao

AU - Merrill, Daniel

AU - An, Ran

AU - Turek, John

AU - Matei, Daniela

AU - Nolte, David D.

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Three-dimensional (3-D) tissue culture represents a more biologically relevant environment for testing new drugs compared to conventional two-dimensional cancer cell culture models. Biodynamic imaging is a high-content 3-D optical imaging technology based on low-coherence interferometry and digital holography that uses dynamic speckle as high-content image contrast to probe deep inside 3-D tissue. Speckle contrast is shown to be a scaling function of the acquisition time relative to the persistence time of intracellular transport and hence provides a measure of cellular activity. Cellular responses of 3-D multicellular spheroids to paclitaxel are compared among three different growth techniques: rotating bioreactor (BR), hanging-drop (HD), and nonadherent (U-bottom, UB) plate spheroids, compared with ex vivo living tissues. HD spheroids have the most homogeneous tissue, whereas BR spheroids display large sample-to-sample variability as well as spatial heterogeneity. The responses of BR-grown tumor spheroids to paclitaxel are more similar to those of ex vivo biopsies than the responses of spheroids grown using HD or plate methods. The rate of mitosis inhibition by application of taxol is measured through tissue dynamics spectroscopic imaging, demonstrating the ability to monitor antimitotic chemotherapy. These results illustrate the potential use of low-coherence digital holography for 3-D pharmaceutical screening applications.

AB - Three-dimensional (3-D) tissue culture represents a more biologically relevant environment for testing new drugs compared to conventional two-dimensional cancer cell culture models. Biodynamic imaging is a high-content 3-D optical imaging technology based on low-coherence interferometry and digital holography that uses dynamic speckle as high-content image contrast to probe deep inside 3-D tissue. Speckle contrast is shown to be a scaling function of the acquisition time relative to the persistence time of intracellular transport and hence provides a measure of cellular activity. Cellular responses of 3-D multicellular spheroids to paclitaxel are compared among three different growth techniques: rotating bioreactor (BR), hanging-drop (HD), and nonadherent (U-bottom, UB) plate spheroids, compared with ex vivo living tissues. HD spheroids have the most homogeneous tissue, whereas BR spheroids display large sample-to-sample variability as well as spatial heterogeneity. The responses of BR-grown tumor spheroids to paclitaxel are more similar to those of ex vivo biopsies than the responses of spheroids grown using HD or plate methods. The rate of mitosis inhibition by application of taxol is measured through tissue dynamics spectroscopic imaging, demonstrating the ability to monitor antimitotic chemotherapy. These results illustrate the potential use of low-coherence digital holography for 3-D pharmaceutical screening applications.

KW - biodynamic imaging

KW - biological relevance

KW - biomedical imaging

KW - cellular dynamics

KW - digital holography

KW - drug development

KW - drug discovery

KW - dynamic light scattering

KW - high-content imaging

KW - low-coherence interferometry

KW - pharmaceutical

KW - physiological effects of paclitaxel

KW - speckle

KW - Three-dimensional tissue growth

UR - http://www.scopus.com/inward/record.url?scp=85009288403&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85009288403&partnerID=8YFLogxK

U2 - 10.1117/1.JBO.22.1.016007

DO - 10.1117/1.JBO.22.1.016007

M3 - Article

C2 - 28301634

AN - SCOPUS:85009288403

VL - 22

JO - Journal of Biomedical Optics

JF - Journal of Biomedical Optics

SN - 1083-3668

IS - 1

M1 - 016007

ER -