Biomimetic approach for root caries prevention using a proanthocyanidin- rich agent

S. Pavan, Q. Xie, A. T. Hara, A. K. Bedran-Russo

Research output: Contribution to journalArticle

27 Scopus citations


The aim of this study was to evaluate the effects of a proanthocyanidin- rich grape seed extract (GSE) on the in vitro demineralization of root dentine. Root fragments were obtained from sound human teeth. The fragments were randomly assigned to different treatments solutions: GSE, fluoride (F), GSE+F and distilled water (control). Samples were treated daily for 30 min and subjected to a pH cycling artificial caries protocol using demineralization cycles (2.2 mM CaCl 2×H 2O, 2.2 mM KH 2PO 4, 50 mM acetic acid, pH 4.3) for 6 h and remineralization cycles (20 mM HEPES, 2.25 mM CaCl 2×H 2O, 1.35 mM KH 2PO 4, 130 mM KCl, pH 7.0) for 17.5 h. Mineral loss (ΔZ) and lesion depth (LD) were determined after 18 days of treatment/pH cycling, by transverse microradiography. GSE was able to minimize ΔZ and LD compared with the control group (p < 0.0001). The GSE+F and F groups showed the lowest values of ΔZ and LD (p < 0.05), with no statistically significant differences between them (p = 0.554 and p = 0.726, respectively). A biomimetic approach to strengthen root dentine using GSE results in decreased rates of root demineralization and may be used in conjunction with F to prevent root caries.

Original languageEnglish (US)
Pages (from-to)443-447
Number of pages5
JournalCaries research
Issue number5
StatePublished - Nov 1 2011


  • Grape seed extract
  • Proanthocyanidin
  • Remineralization
  • Root caries

ASJC Scopus subject areas

  • Dentistry(all)

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