Biophotonic hydrogen peroxide production by antibodies, T cells, and T-cell membranes

Gabi Nindl Waite, Lee R. Waite, Ellen F. Hughes, Walter X. Balcavage

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Rapidly accumulating evidence indicates that inflammatory T cells sensitively respond to their redox environment by activating signal transduction pathways. The hypothesis that T-cell receptors have the potential to catalytically transform singlet oxygen into H2O2 attracted our attention since the biophysical regulation of this process would provide a new tool for therapeutically directing T cells down a preferred signaling pathway. Light-dependent production of H2O2 was first described in antibodies, and we reproduced these findings. Using a real-time H2O2 sensor we extended them by showing that the reaction proceeds in a biphasic way with a short-lived phase that is fast compared to the slow second phase of the reaction. We then showed that Jurkat T cells biophotonically produce about 30 nM H2O2/min/mg protein when pretreated with NaN3. This activity was concentrated 4 to 5 times in T-cell membrane preparations. The implications of these observations for the development of new therapeutic tools for inflammatory diseases are discussed.

Original languageEnglish
Pages (from-to)1110-1117
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume338
Issue number2
DOIs
StatePublished - Dec 16 2005

Fingerprint

T-cells
Cell membranes
Hydrogen Peroxide
Antibody Formation
Cell Membrane
T-Lymphocytes
Antibodies
Biophysical Phenomena
Sodium Azide
Signal transduction
Singlet Oxygen
Jurkat Cells
T-Cell Antigen Receptor
Oxidation-Reduction
Signal Transduction
Light
Sensors
Proteins
Therapeutics

Keywords

  • O
  • HO
  • Photosensitizer
  • Reactive oxygen species
  • Real-time sensor
  • ROS
  • T lymphocytes
  • Ultraviolet light

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Biophotonic hydrogen peroxide production by antibodies, T cells, and T-cell membranes. / Waite, Gabi Nindl; Waite, Lee R.; Hughes, Ellen F.; Balcavage, Walter X.

In: Biochemical and Biophysical Research Communications, Vol. 338, No. 2, 16.12.2005, p. 1110-1117.

Research output: Contribution to journalArticle

Waite, Gabi Nindl ; Waite, Lee R. ; Hughes, Ellen F. ; Balcavage, Walter X. / Biophotonic hydrogen peroxide production by antibodies, T cells, and T-cell membranes. In: Biochemical and Biophysical Research Communications. 2005 ; Vol. 338, No. 2. pp. 1110-1117.
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