Biotin labeling and quantitation of cell-surface proteins.

D. N. Turvy, Janice Blum

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Cell surface receptors, such as transferrin receptors and MHC molecules, are internalized into the endocytic pathway and recycled to the plasma membrane. Previous assays used to measure endocytosis and recycling were cumbersome and often required radioactive reagents. This unit describes protocols that employ the combination of a cleavable biotin reagent to label surface molecules and a capture ELISA to detect these molecules allowing for rapid and safe quantitation of cell surface protein expression, endocytosis, and recycling.

Original languageEnglish
JournalCurrent protocols in immunology / edited by John E. Coligan ... [et al.]
VolumeChapter 18
StatePublished - May 2001

Fingerprint

Recycling
Biotin
Endocytosis
Membrane Proteins
Transferrin Receptors
Cell Surface Receptors
Enzyme-Linked Immunosorbent Assay
Cell Membrane

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Biotin labeling and quantitation of cell-surface proteins. / Turvy, D. N.; Blum, Janice.

In: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 18, 05.2001.

Research output: Contribution to journalArticle

@article{8e1deedee5744db9b0fc8636e34b73e7,
title = "Biotin labeling and quantitation of cell-surface proteins.",
abstract = "Cell surface receptors, such as transferrin receptors and MHC molecules, are internalized into the endocytic pathway and recycled to the plasma membrane. Previous assays used to measure endocytosis and recycling were cumbersome and often required radioactive reagents. This unit describes protocols that employ the combination of a cleavable biotin reagent to label surface molecules and a capture ELISA to detect these molecules allowing for rapid and safe quantitation of cell surface protein expression, endocytosis, and recycling.",
author = "Turvy, {D. N.} and Janice Blum",
year = "2001",
month = "5",
language = "English",
volume = "Chapter 18",
journal = "Current Protocols in Immunology",
issn = "1934-3671",
publisher = "John Wiley and Sons Inc.",

}

TY - JOUR

T1 - Biotin labeling and quantitation of cell-surface proteins.

AU - Turvy, D. N.

AU - Blum, Janice

PY - 2001/5

Y1 - 2001/5

N2 - Cell surface receptors, such as transferrin receptors and MHC molecules, are internalized into the endocytic pathway and recycled to the plasma membrane. Previous assays used to measure endocytosis and recycling were cumbersome and often required radioactive reagents. This unit describes protocols that employ the combination of a cleavable biotin reagent to label surface molecules and a capture ELISA to detect these molecules allowing for rapid and safe quantitation of cell surface protein expression, endocytosis, and recycling.

AB - Cell surface receptors, such as transferrin receptors and MHC molecules, are internalized into the endocytic pathway and recycled to the plasma membrane. Previous assays used to measure endocytosis and recycling were cumbersome and often required radioactive reagents. This unit describes protocols that employ the combination of a cleavable biotin reagent to label surface molecules and a capture ELISA to detect these molecules allowing for rapid and safe quantitation of cell surface protein expression, endocytosis, and recycling.

UR - http://www.scopus.com/inward/record.url?scp=44649084521&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=44649084521&partnerID=8YFLogxK

M3 - Article

C2 - 18432749

AN - SCOPUS:44649084521

VL - Chapter 18

JO - Current Protocols in Immunology

JF - Current Protocols in Immunology

SN - 1934-3671

ER -