Biphasic bisperoxovanadium administration and Schwann cell transplantation for repair after cervical contusive spinal cord injury

Chandler Walker, Xiaofei Wang, Carli Bullis, Naikui Liu, Qingbo Lu, Colin Fry, Lingxiao Deng, Xiao-Ming Xu

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Schwann cells (SCs) hold promise for spinal cord injury (SCI) repair; however, there are limitations for its use as a lone treatment. We showed that acute inhibition of the phosphatase and tensin homolog deleted on chromosome ten (PTEN) by bisperoxovanadium (bpV) was neuroprotective and enhanced function following cervical hemicontusion SCI. We hypothesized that combining acute bpV therapy and delayed SC engraftment would further improve neuroprotection and recovery after cervical SCI. Adult female Sprague-Dawley (SD) rats were randomly sorted into 5 groups: sham, vehicle, bpV, SC transplantation, and bpV+SC transplantation. SCs were isolated from adult green fluorescent protein (GFP)-expressing SD rats (GFP-SCs). 200μg/kg bpV(pic) was administered intraperitoneally (IP) twice daily for 7days post-SCI in bpV-treated groups. GFP-SCs (1×106 in 5μl medium) were transplanted into the lesion epicenter at the 8th day post-SCI. Forelimb function was tested for 10weeks and histology was assessed. bpV alone significantly reduced lesion (by 40%, p<0.05) and cavitation (by 65%, p<0.05) and improved functional recovery (p<0.05) compared to injury alone. The combination promoted similar neuroprotection (p<0.01 vs. injury); however, GFP-SCs alone did not. Both SC-transplanted groups exhibited remarkable long-term SC survival, SMI-31+ axon ingrowth and RECA-1+ vasculature presence in the SC graft; however, bpV+SCs promoted an 89% greater axon-to-lesion ratio than SCs only. We concluded that bpV likely contributed largely to the neuroprotective and functional benefits while SCs facilitated considerable host-tissue interaction and modification. The combination of the two shows promise as an attractive strategy to enhance recovery after SCI.

Original languageEnglish
Pages (from-to)163-172
Number of pages10
JournalExperimental Neurology
Volume264
DOIs
StatePublished - Feb 1 2015

Fingerprint

Schwann Cells
Cell Transplantation
Spinal Cord Injuries
Green Fluorescent Proteins
bisperoxovanadium
Cervical Cord
Sprague Dawley Rats
Axons
Spinal Cord Regeneration
Forelimb
Wounds and Injuries
Phosphoric Monoester Hydrolases
Cell Survival
Histology

Keywords

  • BpV
  • Neuroprotection
  • PTEN
  • Schwann cell transplantation
  • Spinal cord injury

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience

Cite this

Biphasic bisperoxovanadium administration and Schwann cell transplantation for repair after cervical contusive spinal cord injury. / Walker, Chandler; Wang, Xiaofei; Bullis, Carli; Liu, Naikui; Lu, Qingbo; Fry, Colin; Deng, Lingxiao; Xu, Xiao-Ming.

In: Experimental Neurology, Vol. 264, 01.02.2015, p. 163-172.

Research output: Contribution to journalArticle

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