Bisphosphonates do not alter the rate of secondary mineralization

Robyn K. Fuchs, Meghan E. Faillace, Matthew Allen, Roger J. Phipps, Lisa M. Miller, David Burr

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Bisphosphonates function to reduce bone turnover, which consequently increases the mean degree of tissue mineralization at an organ level. However, it is not clear if bisphosphonates alter the length of time required for an individual bone-modeling unit (BMU) to fully mineralize. We have recently demonstrated that it takes ~. 350. days (d) for normal, untreated cortical bone to fully mineralize. The aim of this study was to determine the rate at which newly formed trabecular BMUs become fully mineralized in rabbits treated for up to 414. d with clinical doses of either risedronate (RIS) or alendronate (ALN). Thirty-six, 4-month old virgin female New Zealand white rabbits were allocated to RIS (n=12; 2.4μg/kg body weight), ALN (n=12; 2.4μg/kg body weight), or volume-matched saline controls (CON; n=12). Fluorochrome labels were administered at specific time intervals to quantify the rate and level of mineralization of trabecular bone from the femoral neck (FN) by Fourier transform infrared microspectroscopy (FTIRM). The organic (collagen) and inorganic (phosphate and carbonate) IR spectral characteristics of trabecular bone from undecalcified 4 micron thick tissue sections were quantified from fluorescently labels regions that had mineralized for 1, 8, 18, 35, 70, 105, 140, 210, 280, and 385. d (4 rabbits per time point and treatment group). All groups exhibited a rapid increase in mineralization over the first 18. days, the period of primary mineralization, with no significant differences between treatments. Mineralization continued to increase, at a slower rate up, to 385. days (secondary mineralization), and was not different among treatments. There were no significant differences between treatments for the rate of mineralization within an individual BMU; however, ALN and RIS both increased global tissue mineralization as demonstrated by areal bone mineral density from DXA. We conclude that increases in tissue mineralization that occur following a period of bisphosphonate treatment is a function of the suppressed rate of remodeling that allows for a greater number of BMUs to obtain a greater degree of mineralization.

Original languageEnglish
Pages (from-to)701-705
Number of pages5
JournalBone
Volume49
Issue number4
DOIs
StatePublished - Oct 2011

Fingerprint

Diphosphonates
Alendronate
Rabbits
Body Weight
Bone and Bones
Bone Remodeling
Carbonates
Femur Neck
Fourier Analysis
Fluorescent Dyes
Bone Density
Collagen
Phosphates
Risedronate Sodium
Cancellous Bone

Keywords

  • Animal model
  • Bisphosphonates
  • FTIRM
  • Mineralization
  • Trabecular bone

ASJC Scopus subject areas

  • Physiology
  • Endocrinology, Diabetes and Metabolism
  • Histology

Cite this

Fuchs, R. K., Faillace, M. E., Allen, M., Phipps, R. J., Miller, L. M., & Burr, D. (2011). Bisphosphonates do not alter the rate of secondary mineralization. Bone, 49(4), 701-705. https://doi.org/10.1016/j.bone.2011.05.009

Bisphosphonates do not alter the rate of secondary mineralization. / Fuchs, Robyn K.; Faillace, Meghan E.; Allen, Matthew; Phipps, Roger J.; Miller, Lisa M.; Burr, David.

In: Bone, Vol. 49, No. 4, 10.2011, p. 701-705.

Research output: Contribution to journalArticle

Fuchs, RK, Faillace, ME, Allen, M, Phipps, RJ, Miller, LM & Burr, D 2011, 'Bisphosphonates do not alter the rate of secondary mineralization', Bone, vol. 49, no. 4, pp. 701-705. https://doi.org/10.1016/j.bone.2011.05.009
Fuchs RK, Faillace ME, Allen M, Phipps RJ, Miller LM, Burr D. Bisphosphonates do not alter the rate of secondary mineralization. Bone. 2011 Oct;49(4):701-705. https://doi.org/10.1016/j.bone.2011.05.009
Fuchs, Robyn K. ; Faillace, Meghan E. ; Allen, Matthew ; Phipps, Roger J. ; Miller, Lisa M. ; Burr, David. / Bisphosphonates do not alter the rate of secondary mineralization. In: Bone. 2011 ; Vol. 49, No. 4. pp. 701-705.
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