Bivariate genome-wide association meta-analysis of pediatric musculoskeletal traits reveals pleiotropic effects at the SREBF1/TOM1L2 locus

Carolina Medina-Gomez, John P. Kemp, Niki L. Dimou, Eskil Kreiner, Alessandra Chesi, Babette S. Zemel, Klaus Bønnelykke, Cindy G. Boer, Tarunveer S. Ahluwalia, Hans Bisgaard, Evangelos Evangelou, Denise H.M. Heppe, Lynda Bonewald, Jeffrey P. Gorski, Mohsen Ghanbari, Serkalem Demissie, Gustavo Duque, Matthew T. Maurano, Douglas P. Kiel, Yi Hsiang Hsu & 14 others Bram C.J. Van Der Eerden, Cheryl Ackert-Bicknell, Sjur Reppe, Kaare M. Gautvik, Truls Raastad, David Karasik, Jeroen Van De Peppel, Vincent W.V. Jaddoe, André G. Uitterlinden, Jonathan H. Tobias, Struan F.A. Grant, Pantelis G. Bagos, David M. Evans, Fernando Rivadeneira

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Bone mineral density is known to be a heritable, polygenic trait whereas genetic variants contributing to lean mass variation remain largely unknown. We estimated the shared SNP heritability and performed a bivariate GWAS meta-analysis of total-body lean mass (TB-LM) and total-body less head bone mineral density (TBLH-BMD) regions in 10,414 children. The estimated SNP heritability is 43% (95% CI: 34-52%) for TBLH-BMD, and 39% (95% CI: 30-48%) for TB-LM, with a shared genetic component of 43% (95% CI: 29-56%). We identify variants with pleiotropic effects in eight loci, including seven established bone mineral density loci: WNT4, GALNT3, MEPE, CPED1/WNT16, TNFSF11, RIN3, and PPP6R3/LRP5. Variants in the TOM1L2/SREBF1 locus exert opposing effects TB-LM and TBLH-BMD, and have a stronger association with the former trait. We show that SREBF1 is expressed in murine and human osteoblasts, as well as in human muscle tissue. This is the first bivariate GWAS meta-analysis to demonstrate genetic factors with pleiotropic effects on bone mineral density and lean mass.

Original languageEnglish (US)
Article number121
JournalNature Communications
Volume8
Issue number1
DOIs
StatePublished - Dec 1 2017

Fingerprint

Pediatrics
genome
Genome-Wide Association Study
loci
Bone Density
bones
Minerals
Meta-Analysis
Bone
Genes
minerals
Head
Single Nucleotide Polymorphism
Multifactorial Inheritance
osteoblasts
Osteoblasts
muscles
Muscle
Tissue
Muscles

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Bivariate genome-wide association meta-analysis of pediatric musculoskeletal traits reveals pleiotropic effects at the SREBF1/TOM1L2 locus. / Medina-Gomez, Carolina; Kemp, John P.; Dimou, Niki L.; Kreiner, Eskil; Chesi, Alessandra; Zemel, Babette S.; Bønnelykke, Klaus; Boer, Cindy G.; Ahluwalia, Tarunveer S.; Bisgaard, Hans; Evangelou, Evangelos; Heppe, Denise H.M.; Bonewald, Lynda; Gorski, Jeffrey P.; Ghanbari, Mohsen; Demissie, Serkalem; Duque, Gustavo; Maurano, Matthew T.; Kiel, Douglas P.; Hsu, Yi Hsiang; Van Der Eerden, Bram C.J.; Ackert-Bicknell, Cheryl; Reppe, Sjur; Gautvik, Kaare M.; Raastad, Truls; Karasik, David; Van De Peppel, Jeroen; Jaddoe, Vincent W.V.; Uitterlinden, André G.; Tobias, Jonathan H.; Grant, Struan F.A.; Bagos, Pantelis G.; Evans, David M.; Rivadeneira, Fernando.

In: Nature Communications, Vol. 8, No. 1, 121, 01.12.2017.

Research output: Contribution to journalArticle

Medina-Gomez, C, Kemp, JP, Dimou, NL, Kreiner, E, Chesi, A, Zemel, BS, Bønnelykke, K, Boer, CG, Ahluwalia, TS, Bisgaard, H, Evangelou, E, Heppe, DHM, Bonewald, L, Gorski, JP, Ghanbari, M, Demissie, S, Duque, G, Maurano, MT, Kiel, DP, Hsu, YH, Van Der Eerden, BCJ, Ackert-Bicknell, C, Reppe, S, Gautvik, KM, Raastad, T, Karasik, D, Van De Peppel, J, Jaddoe, VWV, Uitterlinden, AG, Tobias, JH, Grant, SFA, Bagos, PG, Evans, DM & Rivadeneira, F 2017, 'Bivariate genome-wide association meta-analysis of pediatric musculoskeletal traits reveals pleiotropic effects at the SREBF1/TOM1L2 locus', Nature Communications, vol. 8, no. 1, 121. https://doi.org/10.1038/s41467-017-00108-3
Medina-Gomez, Carolina ; Kemp, John P. ; Dimou, Niki L. ; Kreiner, Eskil ; Chesi, Alessandra ; Zemel, Babette S. ; Bønnelykke, Klaus ; Boer, Cindy G. ; Ahluwalia, Tarunveer S. ; Bisgaard, Hans ; Evangelou, Evangelos ; Heppe, Denise H.M. ; Bonewald, Lynda ; Gorski, Jeffrey P. ; Ghanbari, Mohsen ; Demissie, Serkalem ; Duque, Gustavo ; Maurano, Matthew T. ; Kiel, Douglas P. ; Hsu, Yi Hsiang ; Van Der Eerden, Bram C.J. ; Ackert-Bicknell, Cheryl ; Reppe, Sjur ; Gautvik, Kaare M. ; Raastad, Truls ; Karasik, David ; Van De Peppel, Jeroen ; Jaddoe, Vincent W.V. ; Uitterlinden, André G. ; Tobias, Jonathan H. ; Grant, Struan F.A. ; Bagos, Pantelis G. ; Evans, David M. ; Rivadeneira, Fernando. / Bivariate genome-wide association meta-analysis of pediatric musculoskeletal traits reveals pleiotropic effects at the SREBF1/TOM1L2 locus. In: Nature Communications. 2017 ; Vol. 8, No. 1.
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abstract = "Bone mineral density is known to be a heritable, polygenic trait whereas genetic variants contributing to lean mass variation remain largely unknown. We estimated the shared SNP heritability and performed a bivariate GWAS meta-analysis of total-body lean mass (TB-LM) and total-body less head bone mineral density (TBLH-BMD) regions in 10,414 children. The estimated SNP heritability is 43{\%} (95{\%} CI: 34-52{\%}) for TBLH-BMD, and 39{\%} (95{\%} CI: 30-48{\%}) for TB-LM, with a shared genetic component of 43{\%} (95{\%} CI: 29-56{\%}). We identify variants with pleiotropic effects in eight loci, including seven established bone mineral density loci: WNT4, GALNT3, MEPE, CPED1/WNT16, TNFSF11, RIN3, and PPP6R3/LRP5. Variants in the TOM1L2/SREBF1 locus exert opposing effects TB-LM and TBLH-BMD, and have a stronger association with the former trait. We show that SREBF1 is expressed in murine and human osteoblasts, as well as in human muscle tissue. This is the first bivariate GWAS meta-analysis to demonstrate genetic factors with pleiotropic effects on bone mineral density and lean mass.",
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AU - Medina-Gomez, Carolina

AU - Kemp, John P.

AU - Dimou, Niki L.

AU - Kreiner, Eskil

AU - Chesi, Alessandra

AU - Zemel, Babette S.

AU - Bønnelykke, Klaus

AU - Boer, Cindy G.

AU - Ahluwalia, Tarunveer S.

AU - Bisgaard, Hans

AU - Evangelou, Evangelos

AU - Heppe, Denise H.M.

AU - Bonewald, Lynda

AU - Gorski, Jeffrey P.

AU - Ghanbari, Mohsen

AU - Demissie, Serkalem

AU - Duque, Gustavo

AU - Maurano, Matthew T.

AU - Kiel, Douglas P.

AU - Hsu, Yi Hsiang

AU - Van Der Eerden, Bram C.J.

AU - Ackert-Bicknell, Cheryl

AU - Reppe, Sjur

AU - Gautvik, Kaare M.

AU - Raastad, Truls

AU - Karasik, David

AU - Van De Peppel, Jeroen

AU - Jaddoe, Vincent W.V.

AU - Uitterlinden, André G.

AU - Tobias, Jonathan H.

AU - Grant, Struan F.A.

AU - Bagos, Pantelis G.

AU - Evans, David M.

AU - Rivadeneira, Fernando

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