Bleeding the laboratory mouse

Not all methods are equal

Jonathan Hoggatt, Amber F. Hoggatt, Tiffany A. Tate, Jeffrey Fortman, Louis Pelus

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The laboratory mouse is the model most frequently used in hematologic studies and assessment of blood parameters across a broad range of disciplines. Often, analysis of blood occurs in a nonterminal manner. However, the small body size of the mouse limits collection based on volume, frequency, and accessible sites. Commonly used sites in the mouse include the retro-orbital sinus, facial vein, tail vein, saphenous vein, and heart. The method of blood acquisition varies considerably across laboratories and is often not reported in detail. In this study, we report significant alterations in blood parameters, particularly of total white blood cells, specific populations of dendritic cells and myeloid-derived suppressor cells, and hematopoietic progenitor cells, as a result of site and manner of sampling. Intriguingly, warming of mice prior to tail bleeding was found to significantly alter blood values. Our findings suggest that the same method should be used across an entire study, that mice should be warmed prior to tail bleeds to make levels uniform, and that accurate description of bleeding methods in publications should be provided to allow for interpretation of comparative reports and inter- and intralaboratory experimental variability.

Original languageEnglish (US)
Pages (from-to)132-137
Number of pages6
JournalExperimental Hematology
Volume44
Issue number2
DOIs
StatePublished - Feb 1 2016

Fingerprint

Hemorrhage
Tail
Veins
Saphenous Vein
Body Size
Hematopoietic Stem Cells
Dendritic Cells
Publications
Leukocytes
Population

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Hematology

Cite this

Hoggatt, J., Hoggatt, A. F., Tate, T. A., Fortman, J., & Pelus, L. (2016). Bleeding the laboratory mouse: Not all methods are equal. Experimental Hematology, 44(2), 132-137. https://doi.org/10.1016/j.exphem.2015.10.008

Bleeding the laboratory mouse : Not all methods are equal. / Hoggatt, Jonathan; Hoggatt, Amber F.; Tate, Tiffany A.; Fortman, Jeffrey; Pelus, Louis.

In: Experimental Hematology, Vol. 44, No. 2, 01.02.2016, p. 132-137.

Research output: Contribution to journalArticle

Hoggatt, J, Hoggatt, AF, Tate, TA, Fortman, J & Pelus, L 2016, 'Bleeding the laboratory mouse: Not all methods are equal', Experimental Hematology, vol. 44, no. 2, pp. 132-137. https://doi.org/10.1016/j.exphem.2015.10.008
Hoggatt J, Hoggatt AF, Tate TA, Fortman J, Pelus L. Bleeding the laboratory mouse: Not all methods are equal. Experimental Hematology. 2016 Feb 1;44(2):132-137. https://doi.org/10.1016/j.exphem.2015.10.008
Hoggatt, Jonathan ; Hoggatt, Amber F. ; Tate, Tiffany A. ; Fortman, Jeffrey ; Pelus, Louis. / Bleeding the laboratory mouse : Not all methods are equal. In: Experimental Hematology. 2016 ; Vol. 44, No. 2. pp. 132-137.
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