Blockade of interleukin-27 signaling reduces GVHD in mice by augmenting Treg reconstitution and stabilizing Foxp3 expression

Ludovic Belle, Kimberle Agle, Vivian Zhou, Cheng Yin-Yuan, Richard Komorowski, Daniel Eastwood, Brent Logan, Jie Sun, Nico Ghilardi, Daniel Cua, Calvin B. Williams, Melanie Gaignage, Reece Marillier, Jacques van Snick, William R. Drobyski

Research output: Contribution to journalArticle

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Abstract

Reestablishment of competent regulatory pathways has emerged as a strategy to reduce the severity of graft-versus-host disease (GVHD), and recalibrate the effector and regulatory arms of the immune system. However, clinically feasible, cost-effective strategies that do not require extensive ex vivo cellular manipulation have remained elusive. In the current study, we demonstrate that inhibition of the interleukin-27p28 (IL-27p28) signaling pathway through antibody blockade or genetic ablation prevented lethal GVHD in multiple murine transplant models. Moreover, protection from GVHD was attributable to augmented global reconstitution of CD4+ natural regulatory T cells (nTregs), CD4+ induced Tregs (iTregs), and CD8+ iTregs, and was more potent than temporally concordant blockade of IL-6 signaling. Inhibition of IL-27p28 also enhanced the suppressive capacity of adoptively transferred CD4+ nTregs by increasing the stability of Foxp3 expression. Notably, blockade of IL-27p28 signaling reduced T-cell-derived-IL-10 production in conventional T cells; however, there was no corresponding effect in CD4+ or CD8+ Tregs, indicating that IL-27 inhibition had differential effects on IL-10 production and preserved a mechanistic pathway by which Tregs are known to suppress GVHD. Targeting of IL-27 therefore represents a novel strategy for the in vivo expansion of Tregs and subsequent prevention of GVHD without the requirement for ex vivo cellular manipulation, and provides additional support for the critical proinflammatory role that members of the IL-6 and IL-12 cytokine families play in GVHD biology.

Original languageEnglish (US)
Pages (from-to)2068-2082
Number of pages15
JournalBlood
Volume128
Issue number16
DOIs
StatePublished - Oct 20 2016

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Interleukin-27
Graft vs Host Disease
Grafts
T-cells
Interleukins
Interleukin-10
Interleukin-6
T-Lymphocytes
Transplants
Immune system
Regulatory T-Lymphocytes
Interleukin-12
Ablation
Immune System
Cytokines
Costs and Cost Analysis
Antibodies

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Belle, L., Agle, K., Zhou, V., Yin-Yuan, C., Komorowski, R., Eastwood, D., ... Drobyski, W. R. (2016). Blockade of interleukin-27 signaling reduces GVHD in mice by augmenting Treg reconstitution and stabilizing Foxp3 expression. Blood, 128(16), 2068-2082. https://doi.org/10.1182/blood-2016-02-698241

Blockade of interleukin-27 signaling reduces GVHD in mice by augmenting Treg reconstitution and stabilizing Foxp3 expression. / Belle, Ludovic; Agle, Kimberle; Zhou, Vivian; Yin-Yuan, Cheng; Komorowski, Richard; Eastwood, Daniel; Logan, Brent; Sun, Jie; Ghilardi, Nico; Cua, Daniel; Williams, Calvin B.; Gaignage, Melanie; Marillier, Reece; van Snick, Jacques; Drobyski, William R.

In: Blood, Vol. 128, No. 16, 20.10.2016, p. 2068-2082.

Research output: Contribution to journalArticle

Belle, L, Agle, K, Zhou, V, Yin-Yuan, C, Komorowski, R, Eastwood, D, Logan, B, Sun, J, Ghilardi, N, Cua, D, Williams, CB, Gaignage, M, Marillier, R, van Snick, J & Drobyski, WR 2016, 'Blockade of interleukin-27 signaling reduces GVHD in mice by augmenting Treg reconstitution and stabilizing Foxp3 expression', Blood, vol. 128, no. 16, pp. 2068-2082. https://doi.org/10.1182/blood-2016-02-698241
Belle, Ludovic ; Agle, Kimberle ; Zhou, Vivian ; Yin-Yuan, Cheng ; Komorowski, Richard ; Eastwood, Daniel ; Logan, Brent ; Sun, Jie ; Ghilardi, Nico ; Cua, Daniel ; Williams, Calvin B. ; Gaignage, Melanie ; Marillier, Reece ; van Snick, Jacques ; Drobyski, William R. / Blockade of interleukin-27 signaling reduces GVHD in mice by augmenting Treg reconstitution and stabilizing Foxp3 expression. In: Blood. 2016 ; Vol. 128, No. 16. pp. 2068-2082.
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AU - Belle, Ludovic

AU - Agle, Kimberle

AU - Zhou, Vivian

AU - Yin-Yuan, Cheng

AU - Komorowski, Richard

AU - Eastwood, Daniel

AU - Logan, Brent

AU - Sun, Jie

AU - Ghilardi, Nico

AU - Cua, Daniel

AU - Williams, Calvin B.

AU - Gaignage, Melanie

AU - Marillier, Reece

AU - van Snick, Jacques

AU - Drobyski, William R.

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