Blockade of NADPH oxidase restores vasoreparative function in diabetic CD34 + cells

Yagna P R Jarajapu, Sergio Caballero, Amrisha Verma, Takahiko Nakagawa, Margaret C. Lo, Qiuhong Li, Maria B. Grant

Research output: Contribution to journalArticle

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Abstract

Purpose. The vasodegenerative phase of diabetic retinopathy is likely caused by endothelial dysfunction and reduced endothelial repair. Migration of endothelial progenitor cells (EPCs) into areas of vascular injury is critical to vascular repair. This key function, often defective in diabetes, is largely mediated by nitric oxide (NO), which is known to be inactivated by superoxide produced by NADPH oxidase. The authors tested the hypothesis that either increasing eNOS expression or inhibiting NADPH oxidase would restore the reparative function in diabetic EPCs. Methods. Peripheral blood was obtained from healthy (n = 27) and diabetic (n = 31) persons, and CD34 + cells were isolated. Expression and activation of eNOS and NADPH oxidase and intracellular levels of NO, superoxide, and peroxynitrite were evaluated. cGMP production and migration to SDF-1α were also determined. Reparative function was evaluated in a mouse model of retinal ischemia-reperfusion injury. Results. Diabetic EPCs demonstrate reduced eNOS expression and decreased NO bioavailability and migration in response to SDF-1α. Increasing eNOS expression in diabetic cells by AVE3085 resulted in increased peroxynitrite levels and, therefore, did not enhance NO-mediated functions in vitro and in vivo. Expression of Nox2, NADPH oxidase activity, and superoxide levels were higher in diabetic than in nondiabetic EPCs. Pretreatment with apocynin or gp91ds-tat increased NO bioavailability without increasing eNOS activity in response to SDF-1α. Ex vivo NADPH oxidase inhibition in diabetic cells restored migratory function in vitro and enhanced their homing to ischemic retinal vasculature in vivo. Conclusions. The NADPH oxidase system is a promising target for correcting vasoreparative dysfunction in diabetic EPCs.

Original languageEnglish (US)
Pages (from-to)5093-5104
Number of pages12
JournalInvestigative Ophthalmology and Visual Science
Volume52
Issue number8
DOIs
StatePublished - Jul 2011
Externally publishedYes

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NADPH Oxidase
Nitric Oxide
Superoxides
Peroxynitrous Acid
Biological Availability
Vascular System Injuries
Diabetic Retinopathy
Reperfusion Injury
Blood Vessels
Endothelial Progenitor Cells

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience
  • Medicine(all)

Cite this

Jarajapu, Y. P. R., Caballero, S., Verma, A., Nakagawa, T., Lo, M. C., Li, Q., & Grant, M. B. (2011). Blockade of NADPH oxidase restores vasoreparative function in diabetic CD34 + cells. Investigative Ophthalmology and Visual Science, 52(8), 5093-5104. https://doi.org/10.1167/iovs.10-70911

Blockade of NADPH oxidase restores vasoreparative function in diabetic CD34 + cells. / Jarajapu, Yagna P R; Caballero, Sergio; Verma, Amrisha; Nakagawa, Takahiko; Lo, Margaret C.; Li, Qiuhong; Grant, Maria B.

In: Investigative Ophthalmology and Visual Science, Vol. 52, No. 8, 07.2011, p. 5093-5104.

Research output: Contribution to journalArticle

Jarajapu, YPR, Caballero, S, Verma, A, Nakagawa, T, Lo, MC, Li, Q & Grant, MB 2011, 'Blockade of NADPH oxidase restores vasoreparative function in diabetic CD34 + cells', Investigative Ophthalmology and Visual Science, vol. 52, no. 8, pp. 5093-5104. https://doi.org/10.1167/iovs.10-70911
Jarajapu, Yagna P R ; Caballero, Sergio ; Verma, Amrisha ; Nakagawa, Takahiko ; Lo, Margaret C. ; Li, Qiuhong ; Grant, Maria B. / Blockade of NADPH oxidase restores vasoreparative function in diabetic CD34 + cells. In: Investigative Ophthalmology and Visual Science. 2011 ; Vol. 52, No. 8. pp. 5093-5104.
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AU - Lo, Margaret C.

AU - Li, Qiuhong

AU - Grant, Maria B.

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N2 - Purpose. The vasodegenerative phase of diabetic retinopathy is likely caused by endothelial dysfunction and reduced endothelial repair. Migration of endothelial progenitor cells (EPCs) into areas of vascular injury is critical to vascular repair. This key function, often defective in diabetes, is largely mediated by nitric oxide (NO), which is known to be inactivated by superoxide produced by NADPH oxidase. The authors tested the hypothesis that either increasing eNOS expression or inhibiting NADPH oxidase would restore the reparative function in diabetic EPCs. Methods. Peripheral blood was obtained from healthy (n = 27) and diabetic (n = 31) persons, and CD34 + cells were isolated. Expression and activation of eNOS and NADPH oxidase and intracellular levels of NO, superoxide, and peroxynitrite were evaluated. cGMP production and migration to SDF-1α were also determined. Reparative function was evaluated in a mouse model of retinal ischemia-reperfusion injury. Results. Diabetic EPCs demonstrate reduced eNOS expression and decreased NO bioavailability and migration in response to SDF-1α. Increasing eNOS expression in diabetic cells by AVE3085 resulted in increased peroxynitrite levels and, therefore, did not enhance NO-mediated functions in vitro and in vivo. Expression of Nox2, NADPH oxidase activity, and superoxide levels were higher in diabetic than in nondiabetic EPCs. Pretreatment with apocynin or gp91ds-tat increased NO bioavailability without increasing eNOS activity in response to SDF-1α. Ex vivo NADPH oxidase inhibition in diabetic cells restored migratory function in vitro and enhanced their homing to ischemic retinal vasculature in vivo. Conclusions. The NADPH oxidase system is a promising target for correcting vasoreparative dysfunction in diabetic EPCs.

AB - Purpose. The vasodegenerative phase of diabetic retinopathy is likely caused by endothelial dysfunction and reduced endothelial repair. Migration of endothelial progenitor cells (EPCs) into areas of vascular injury is critical to vascular repair. This key function, often defective in diabetes, is largely mediated by nitric oxide (NO), which is known to be inactivated by superoxide produced by NADPH oxidase. The authors tested the hypothesis that either increasing eNOS expression or inhibiting NADPH oxidase would restore the reparative function in diabetic EPCs. Methods. Peripheral blood was obtained from healthy (n = 27) and diabetic (n = 31) persons, and CD34 + cells were isolated. Expression and activation of eNOS and NADPH oxidase and intracellular levels of NO, superoxide, and peroxynitrite were evaluated. cGMP production and migration to SDF-1α were also determined. Reparative function was evaluated in a mouse model of retinal ischemia-reperfusion injury. Results. Diabetic EPCs demonstrate reduced eNOS expression and decreased NO bioavailability and migration in response to SDF-1α. Increasing eNOS expression in diabetic cells by AVE3085 resulted in increased peroxynitrite levels and, therefore, did not enhance NO-mediated functions in vitro and in vivo. Expression of Nox2, NADPH oxidase activity, and superoxide levels were higher in diabetic than in nondiabetic EPCs. Pretreatment with apocynin or gp91ds-tat increased NO bioavailability without increasing eNOS activity in response to SDF-1α. Ex vivo NADPH oxidase inhibition in diabetic cells restored migratory function in vitro and enhanced their homing to ischemic retinal vasculature in vivo. Conclusions. The NADPH oxidase system is a promising target for correcting vasoreparative dysfunction in diabetic EPCs.

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