Ruminococcus gnavus is a Gram positive, nonspore-forming obligate anaerobe normally found in the human alimentary tract. In culture, this organism constitutively produces a 1-3 α-galactosidase. We fractionated and characterized this enzyme demonstrating hydrolysis of the B epitope on erythrocyte membranes and seroconversion to H epitope (blood type O). Since the enzyme yield was low, cell suspension studies could not be performed. Instead, hydrolysis of the B membrane epitope was studied with an ELISA. A highly purified enzyme product was analyzed for characteristics such as pH, ionic strength, and temperature optimum. Activity in red cell preservative solutions and in the presence of type B plasma was also demonstrated. Ruminococcus gnavus a 1-3 α-galactosidase has potential application in the enzymatic conversion of type B to O packed red blood cell units.
|Original language||English (US)|
|Number of pages||12|
|Journal||Artificial Cells, Blood Substitutes, and Immobilization Biotechnology|
|State||Published - Jul 7 2004|
- B epitope
ASJC Scopus subject areas
- Biomedical Engineering