Blood group B degrading activity of Ruminococcus gnavus α-galactosidase

D. Jane Hata, Daniel Smith

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Ruminococcus gnavus is a Gram positive, nonspore-forming obligate anaerobe normally found in the human alimentary tract. In culture, this organism constitutively produces a 1-3 α-galactosidase. We fractionated and characterized this enzyme demonstrating hydrolysis of the B epitope on erythrocyte membranes and seroconversion to H epitope (blood type O). Since the enzyme yield was low, cell suspension studies could not be performed. Instead, hydrolysis of the B membrane epitope was studied with an ELISA. A highly purified enzyme product was analyzed for characteristics such as pH, ionic strength, and temperature optimum. Activity in red cell preservative solutions and in the presence of type B plasma was also demonstrated. Ruminococcus gnavus a 1-3 α-galactosidase has potential application in the enzymatic conversion of type B to O packed red blood cell units.

Original languageEnglish (US)
Pages (from-to)263-274
Number of pages12
JournalArtificial Cells, Blood Substitutes, and Immobilization Biotechnology
Volume32
Issue number2
DOIs
StatePublished - 2004
Externally publishedYes

Fingerprint

Ruminococcus
Galactosidases
Epitopes
Blood Group Antigens
Blood
Enzymes
Hydrolysis
Cells
Membranes
Erythrocyte Membrane
Ionic strength
Osmolar Concentration
Suspensions
Erythrocytes
Enzyme-Linked Immunosorbent Assay
Plasmas
Temperature

Keywords

  • α-galactosidase
  • B epitope

ASJC Scopus subject areas

  • Biomedical Engineering
  • Hematology
  • Biotechnology
  • Biomaterials

Cite this

Blood group B degrading activity of Ruminococcus gnavus α-galactosidase. / Hata, D. Jane; Smith, Daniel.

In: Artificial Cells, Blood Substitutes, and Immobilization Biotechnology, Vol. 32, No. 2, 2004, p. 263-274.

Research output: Contribution to journalArticle

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