Blood vessel wall-derived endothelial colony-forming cells enhance fracture repair and bone regeneration

Kaarthik S. Chandrasekhar, Hongkang Zhou, Pingyu Zeng, Daniel Alge, Wenyao Li, Brandt A. Finney, Mervin Yoder, Jiliang Li

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Endochondral bone formation requires new blood vessel formation, and endothelial progenitor cells (EPCs) may play a role in this process. Endothelial colonyforming cells (ECFCs), one subtype of EPCs, isolated from the microvasculature of rat lungs, exhibited cell surface antigen markers and gene products characteristic of endothelial cells and displayed high proliferative potential and an ability to form vessel-like network structures in vitro. The aim of this study was to evaluate whether ECFCs facilitate bone healing during fracture repair and stimulate bone regeneration. When type I collagen sponge containing ECFCs were surgically wrapped around the fractured femurs of rats, newly formed bone mineral at the site of fracture was 13% greater (P = 0.01) and energy to failure was 46% greater (P = 0.01) compared to sponge-wrapped fractures without ECFCs. When ECFCs in type I collagen sponge were surgically implanted into the bone defective area, more new vessels formed locally in comparison with sponge-alone controls and new bone tissues were seen. Further, co-implantation of ECFCs and hydroxyapatite/tricalcium phosphate (HA/TCP) scaffolds at the bone defective sites stimulated more new bone tissues than HA/TCP scaffold alone. These results show that cell therapy with vessel wall-derived ECFCs can induce new vessel formation, stimulate new bone formation, and facilitate bone repair and could be a useful approach to treat nonunion fractures and bone defects.

Original languageEnglish
Pages (from-to)347-357
Number of pages11
JournalCalcified Tissue International
Volume89
Issue number5
DOIs
StatePublished - Nov 2011

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Bone Regeneration
Blood Vessels
Endothelial Cells
Bone and Bones
Porifera
Durapatite
Collagen Type I
Osteogenesis
Fracture Healing
Bone Fractures
Surface Antigens
Cell- and Tissue-Based Therapy
Microvessels
Femur
Minerals
Lung

Keywords

  • Bone formation
  • Cell therapy
  • Endothelial progenitor cell
  • Fracture repair
  • In vivo
  • Mechanical testing

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Blood vessel wall-derived endothelial colony-forming cells enhance fracture repair and bone regeneration. / Chandrasekhar, Kaarthik S.; Zhou, Hongkang; Zeng, Pingyu; Alge, Daniel; Li, Wenyao; Finney, Brandt A.; Yoder, Mervin; Li, Jiliang.

In: Calcified Tissue International, Vol. 89, No. 5, 11.2011, p. 347-357.

Research output: Contribution to journalArticle

Chandrasekhar, Kaarthik S. ; Zhou, Hongkang ; Zeng, Pingyu ; Alge, Daniel ; Li, Wenyao ; Finney, Brandt A. ; Yoder, Mervin ; Li, Jiliang. / Blood vessel wall-derived endothelial colony-forming cells enhance fracture repair and bone regeneration. In: Calcified Tissue International. 2011 ; Vol. 89, No. 5. pp. 347-357.
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