Blood volume and red cell space in tissues of the rainbow trout, Salmo gairdneri

D. W. Duff, D. Fitzgerald, D. Kullman, D. W. Lipke, J. Ward, Kenneth Olson

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

1. 1. Whole body blood volume and red cell space of 22 tissues were measured in unanesthetized rainbow trout at 4, 12, 30, 60, 150 and 240 min after dorsal aortic injection of 51Cr-labeled red blood cells. 2. 2. Apparent blood volume decreased during the initial 30 min after injection and increased thereafter. At 240 min the blood volume was 33.5 ± 3.1 ml/kg body wt. 3. 3. Tissue red cell space varied as a function of the interval between labeled red cell injection and tissue collection. Red cell space was highest in spleen followed by heart, kidney and liver. Lowest red cell spaces were found in stomach and red and white skeletal muscle. 4. 4. Variability in blood volume and tissue red cell space over time suggests that caution should be exercised in the design of experiments that employ indicator dilution measurements to measure vascular volumes.

Original languageEnglish
Pages (from-to)393-398
Number of pages6
JournalComparative Biochemistry and Physiology -- Part A: Physiology
Volume87
Issue number2
DOIs
StatePublished - 1987

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Oncorhynchus mykiss
Blood Volume
Blood
Cells
Tissue
Injections
Blood Vessels
Stomach
Skeletal Muscle
Liver
Design of experiments
Spleen
Dilution
Erythrocytes
Muscle
Kidney

ASJC Scopus subject areas

  • Physiology
  • Molecular Biology
  • Biochemistry

Cite this

Blood volume and red cell space in tissues of the rainbow trout, Salmo gairdneri. / Duff, D. W.; Fitzgerald, D.; Kullman, D.; Lipke, D. W.; Ward, J.; Olson, Kenneth.

In: Comparative Biochemistry and Physiology -- Part A: Physiology, Vol. 87, No. 2, 1987, p. 393-398.

Research output: Contribution to journalArticle

Duff, D. W. ; Fitzgerald, D. ; Kullman, D. ; Lipke, D. W. ; Ward, J. ; Olson, Kenneth. / Blood volume and red cell space in tissues of the rainbow trout, Salmo gairdneri. In: Comparative Biochemistry and Physiology -- Part A: Physiology. 1987 ; Vol. 87, No. 2. pp. 393-398.
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