Blue light-induced reactivity of retinal age pigment

In vitro generation of oxygen-reactive species

Malgorzata Róz̀anowska, John Jarvis-Evans, Witold Korytowski, Mike E. Boulton, Janice M. Burke, Tadeusz Sarna

Research output: Contribution to journalArticle

320 Citations (Scopus)

Abstract

Exposure of the eye to intense light, particularly blue light, can cause irreversible, oxygen-dependent damage to the retina. However, no key chromophores that trigger such photooxidative processes have been identified yet. We have found that illumination of human retinal pigment epithelium (RPE) cells with light induces significant uptake of oxygen that is both wavelength- and age-dependent. Analysis of photoreactivity of RPE cells and their age pigment lipofuscin indicates that the observed photoreactivity in RPE cells is primarily due to the presence of lipofuscin, which, under aerobic conditions, generates several oxygen-reactive species including singlet oxygen, superoxide anion, and hydrogen peroxide. We have also found that lipofuscin-photosensitized aerobic reactions lead to enhanced lipid peroxidation as measured by accumulation of lipid hydroperoxides and malondialdehyde in illuminated pigment granules. Hydrogen peroxide is only a minor product of aerobic photoexcitation of lipofuscin. We postulate that lipofuscin is a potential photosensitizer that may increase the risk of retinal photodamage and contribute to the development of age-related maculopathy.

Original languageEnglish (US)
Pages (from-to)18825-18830
Number of pages6
JournalJournal of Biological Chemistry
Volume270
Issue number32
StatePublished - Aug 11 1995
Externally publishedYes

Fingerprint

Lipofuscin
Retinal Pigments
Pigments
Reactive Oxygen Species
Retinal Pigment Epithelium
Light
Photoreactivity
Hydrogen Peroxide
Oxygen
Singlet Oxygen
Photosensitizing Agents
Photoexcitation
Lipid Peroxides
Macular Degeneration
Chromophores
Malondialdehyde
Lighting
Superoxides
Lipid Peroxidation
Retina

ASJC Scopus subject areas

  • Biochemistry

Cite this

Róz̀anowska, M., Jarvis-Evans, J., Korytowski, W., Boulton, M. E., Burke, J. M., & Sarna, T. (1995). Blue light-induced reactivity of retinal age pigment: In vitro generation of oxygen-reactive species. Journal of Biological Chemistry, 270(32), 18825-18830.

Blue light-induced reactivity of retinal age pigment : In vitro generation of oxygen-reactive species. / Róz̀anowska, Malgorzata; Jarvis-Evans, John; Korytowski, Witold; Boulton, Mike E.; Burke, Janice M.; Sarna, Tadeusz.

In: Journal of Biological Chemistry, Vol. 270, No. 32, 11.08.1995, p. 18825-18830.

Research output: Contribution to journalArticle

Róz̀anowska, M, Jarvis-Evans, J, Korytowski, W, Boulton, ME, Burke, JM & Sarna, T 1995, 'Blue light-induced reactivity of retinal age pigment: In vitro generation of oxygen-reactive species', Journal of Biological Chemistry, vol. 270, no. 32, pp. 18825-18830.
Róz̀anowska M, Jarvis-Evans J, Korytowski W, Boulton ME, Burke JM, Sarna T. Blue light-induced reactivity of retinal age pigment: In vitro generation of oxygen-reactive species. Journal of Biological Chemistry. 1995 Aug 11;270(32):18825-18830.
Róz̀anowska, Malgorzata ; Jarvis-Evans, John ; Korytowski, Witold ; Boulton, Mike E. ; Burke, Janice M. ; Sarna, Tadeusz. / Blue light-induced reactivity of retinal age pigment : In vitro generation of oxygen-reactive species. In: Journal of Biological Chemistry. 1995 ; Vol. 270, No. 32. pp. 18825-18830.
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