Blue light-induced singlet oxygen generation by retinal lipofuscin in non-polar media

Małgorzata Rózanowska, Jurina Wessels, Mike Boulton, Janice M. Burke, Michael A J Rodgers, T. George Truscott, Tadeusz Sarna

Research output: Contribution to journalArticle

159 Citations (Scopus)

Abstract

Accumulation of lipofuscin (LF) is a prominent feature of aging in the human retinal pigment epithelium (RPE) cells. This age pigment exhibits substantial photoreactivity, which may increase the risk of retinal photodamage and contribute to age-related maculopathy. In a previous study, we detected singlet oxygen generation by lipofuscin granules excited with blue light. In this paper we investigated the ability of hydrophobic components of lipofuscin to photogenerate singlet oxygen in non-polar environments. Singlet oxygen was detected directly by monitoring its characteristic phosphorescence at ca 1270 nm. The action spectrum of singlet oxygen formation indicated that this process was strongly wavelength- dependent and its efficiency decreased with increasing wavelength by a factor of ten, comparing 420 nm and 520 nm. The quantum yield of singlet oxygen increased with increasing concentration of oxygen. Using laser flash photolysis we studied the possible mechanism of singlet oxygen formation. The observed transient, with a broad absorption spectrum peaking at around 440 nm, was identified as a triplet with lifetime ca 11 μs. It was quenched by both molecular oxygen and β-carotene with concomitant formation of a β- carotene triplet state. These results indicate the potential role of hydrophobic components of lipofuscin in blue light-induced damage to the RPE.

Original languageEnglish (US)
Pages (from-to)1107-1112
Number of pages6
JournalFree Radical Biology and Medicine
Volume24
Issue number7-8
DOIs
StatePublished - May 1998
Externally publishedYes

Fingerprint

Lipofuscin
Singlet Oxygen
Light
Retinal Pigments
Retinal Pigment Epithelium
Carotenoids
Photoreactivity
Oxygen
Wavelength
Phosphorescence
Molecular oxygen
Photolysis
Macular Degeneration
Quantum yield
Pigments
Absorption spectra
Lasers
Aging of materials
Monitoring

Keywords

  • Action spectrum
  • Age-related maculopathy
  • Blue light
  • Extractable chromophores
  • Free radical
  • Phototoxicity
  • Retinal lipofuscin
  • Singlet oxygen

ASJC Scopus subject areas

  • Medicine(all)
  • Toxicology
  • Clinical Biochemistry

Cite this

Rózanowska, M., Wessels, J., Boulton, M., Burke, J. M., Rodgers, M. A. J., Truscott, T. G., & Sarna, T. (1998). Blue light-induced singlet oxygen generation by retinal lipofuscin in non-polar media. Free Radical Biology and Medicine, 24(7-8), 1107-1112. https://doi.org/10.1016/S0891-5849(97)00395-X

Blue light-induced singlet oxygen generation by retinal lipofuscin in non-polar media. / Rózanowska, Małgorzata; Wessels, Jurina; Boulton, Mike; Burke, Janice M.; Rodgers, Michael A J; Truscott, T. George; Sarna, Tadeusz.

In: Free Radical Biology and Medicine, Vol. 24, No. 7-8, 05.1998, p. 1107-1112.

Research output: Contribution to journalArticle

Rózanowska, M, Wessels, J, Boulton, M, Burke, JM, Rodgers, MAJ, Truscott, TG & Sarna, T 1998, 'Blue light-induced singlet oxygen generation by retinal lipofuscin in non-polar media', Free Radical Biology and Medicine, vol. 24, no. 7-8, pp. 1107-1112. https://doi.org/10.1016/S0891-5849(97)00395-X
Rózanowska M, Wessels J, Boulton M, Burke JM, Rodgers MAJ, Truscott TG et al. Blue light-induced singlet oxygen generation by retinal lipofuscin in non-polar media. Free Radical Biology and Medicine. 1998 May;24(7-8):1107-1112. https://doi.org/10.1016/S0891-5849(97)00395-X
Rózanowska, Małgorzata ; Wessels, Jurina ; Boulton, Mike ; Burke, Janice M. ; Rodgers, Michael A J ; Truscott, T. George ; Sarna, Tadeusz. / Blue light-induced singlet oxygen generation by retinal lipofuscin in non-polar media. In: Free Radical Biology and Medicine. 1998 ; Vol. 24, No. 7-8. pp. 1107-1112.
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