Bmi1 promotes erythroid development through regulating ribosome biogenesis

Rui Gao, Sisi Chen, Michihiro Kobayashi, Hao Yu, Yingchi Zhang, Yang Wan, Sara K. Young, Anthony Soltis, Ming Yu, Sasidhar Vemula, Ernest Fraenkel, Alan Cantor, Yevgeniy Antipin, Yang Xu, Mervin Yoder, Ronald Wek, Steven R. Ellis, Reuben Kapur, Xiaofan Zhu, Yan Liu

Research output: Contribution to journalArticle

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Abstract

While Polycomb group protein Bmi1 is important for stem cell maintenance, its role in lineage commitment is largely unknown. We have identified Bmi1 as a novel regulator of erythroid development. Bmi1 is highly expressed in mouse erythroid progenitor cells and its deficiency impairs erythroid differentiation. BMI1 is also important for human erythroid development. Furthermore, we discovered that loss of Bmi1 in erythroid progenitor cells results in decreased transcription of multiple ribosomal protein genes and impaired ribosome biogenesis. Bmi1 deficiency stabilizes p53 protein, leading to upregulation of p21 expression and subsequent G0/G1 cell cycle arrest. Genetic inhibition of p53 activity rescues the erythroid defects seen in the Bmi1 null mice, demonstrating that a p53-dependent mechanism underlies the pathophysiology of the anemia. Mechanistically, Bmi1 is associated with multiple ribosomal protein genes and may positively regulate their expression in erythroid progenitor cells. Thus, Bmi1 promotes erythroid development, at least in part through regulating ribosome biogenesis. Ribosomopathies are human disorders of ribosome dysfunction, including Diamond-Blackfan anemia (DBA) and 5q- syndrome, in which genetic abnormalities cause impaired ribosome biogenesis, resulting in specific clinical phenotypes. We observed that BMI1 expression in human hematopoietic stem and progenitor cells from patients with DBA is correlated with the expression of some ribosomal protein genes, suggesting that BMI1 deficiency may play a pathological role in DBA and other ribosomopathies. Stem Cells 2015;33:925-938

Original languageEnglish
Pages (from-to)925-938
Number of pages14
JournalStem Cells
Volume33
Issue number3
DOIs
StatePublished - Mar 1 2015

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Diamond-Blackfan Anemia
Ribosomes
Erythroid Precursor Cells
Ribosomal Proteins
Hematopoietic Stem Cells
Stem Cells
Polycomb-Group Proteins
Genes
G1 Phase Cell Cycle Checkpoints
Human Development
Anemia
Up-Regulation
Maintenance
Phenotype
Proteins

Keywords

  • Bmi1
  • Erythroid differentiation
  • p53
  • Ribosome biogenesis
  • Ribosomopathies

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Molecular Medicine

Cite this

Gao, R., Chen, S., Kobayashi, M., Yu, H., Zhang, Y., Wan, Y., ... Liu, Y. (2015). Bmi1 promotes erythroid development through regulating ribosome biogenesis. Stem Cells, 33(3), 925-938. https://doi.org/10.1002/stem.1896

Bmi1 promotes erythroid development through regulating ribosome biogenesis. / Gao, Rui; Chen, Sisi; Kobayashi, Michihiro; Yu, Hao; Zhang, Yingchi; Wan, Yang; Young, Sara K.; Soltis, Anthony; Yu, Ming; Vemula, Sasidhar; Fraenkel, Ernest; Cantor, Alan; Antipin, Yevgeniy; Xu, Yang; Yoder, Mervin; Wek, Ronald; Ellis, Steven R.; Kapur, Reuben; Zhu, Xiaofan; Liu, Yan.

In: Stem Cells, Vol. 33, No. 3, 01.03.2015, p. 925-938.

Research output: Contribution to journalArticle

Gao, R, Chen, S, Kobayashi, M, Yu, H, Zhang, Y, Wan, Y, Young, SK, Soltis, A, Yu, M, Vemula, S, Fraenkel, E, Cantor, A, Antipin, Y, Xu, Y, Yoder, M, Wek, R, Ellis, SR, Kapur, R, Zhu, X & Liu, Y 2015, 'Bmi1 promotes erythroid development through regulating ribosome biogenesis', Stem Cells, vol. 33, no. 3, pp. 925-938. https://doi.org/10.1002/stem.1896
Gao R, Chen S, Kobayashi M, Yu H, Zhang Y, Wan Y et al. Bmi1 promotes erythroid development through regulating ribosome biogenesis. Stem Cells. 2015 Mar 1;33(3):925-938. https://doi.org/10.1002/stem.1896
Gao, Rui ; Chen, Sisi ; Kobayashi, Michihiro ; Yu, Hao ; Zhang, Yingchi ; Wan, Yang ; Young, Sara K. ; Soltis, Anthony ; Yu, Ming ; Vemula, Sasidhar ; Fraenkel, Ernest ; Cantor, Alan ; Antipin, Yevgeniy ; Xu, Yang ; Yoder, Mervin ; Wek, Ronald ; Ellis, Steven R. ; Kapur, Reuben ; Zhu, Xiaofan ; Liu, Yan. / Bmi1 promotes erythroid development through regulating ribosome biogenesis. In: Stem Cells. 2015 ; Vol. 33, No. 3. pp. 925-938.
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