BMP-9-transduced prefabricated muscular flaps for the treatment of bony defects.

Mohamed M. Abdelaal, Sunil S. Tholpady, Jessica D. Kessler, Raymond F. Morgan, Roy C. Ogle

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Autologous bone grafting techniques involve the use of tissues that need to be extracted from healthy sites. This can lead to significant donor site morbidity that causes a one-site defect to become a two-site defect. Bone grafts can be especially difficult to manipulate, because bone is a relatively nonmoldable tissue. Furthermore, the inability of a bone graft to contain a transplantable vascular supply also limits the possible size that such a bone graft can be. Because of these limitations, a graft that was moldable with a vascular supply would possess significant advantages in reconstructive applications. In this research, gene therapy techniques were used to create such a graft. An adenovirus expressing BMP-9 was injected into the latissimus dorsi of a nude animal to cause bony differentiation of that muscle. Differentiation of the muscle to cartilage in bone was measured by reverse transcription polymerase chain reaction and immunohistochemistry to determine the optimal time of flap elevation. After injection of the BMP-9 virus, the animals were biopsied weekly over a 3-week period. Both bone and cartilage markers were discovered in these tissues over the study period. Optimal flap elevation time was established to be 2 weeks after injection of the virus.

Original languageEnglish (US)
JournalJournal of Craniofacial Surgery
Volume15
Issue number5
StatePublished - Sep 2004
Externally publishedYes

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Growth Differentiation Factor 2
Bone and Bones
Transplants
Cartilage
Blood Vessels
Therapeutics
Viruses
Muscles
Injections
Superficial Back Muscles
Bone Transplantation
Adenoviridae
Genetic Therapy
Reverse Transcription
Immunohistochemistry
Morbidity
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Surgery

Cite this

Abdelaal, M. M., Tholpady, S. S., Kessler, J. D., Morgan, R. F., & Ogle, R. C. (2004). BMP-9-transduced prefabricated muscular flaps for the treatment of bony defects. Journal of Craniofacial Surgery, 15(5).

BMP-9-transduced prefabricated muscular flaps for the treatment of bony defects. / Abdelaal, Mohamed M.; Tholpady, Sunil S.; Kessler, Jessica D.; Morgan, Raymond F.; Ogle, Roy C.

In: Journal of Craniofacial Surgery, Vol. 15, No. 5, 09.2004.

Research output: Contribution to journalArticle

Abdelaal, MM, Tholpady, SS, Kessler, JD, Morgan, RF & Ogle, RC 2004, 'BMP-9-transduced prefabricated muscular flaps for the treatment of bony defects.', Journal of Craniofacial Surgery, vol. 15, no. 5.
Abdelaal MM, Tholpady SS, Kessler JD, Morgan RF, Ogle RC. BMP-9-transduced prefabricated muscular flaps for the treatment of bony defects. Journal of Craniofacial Surgery. 2004 Sep;15(5).
Abdelaal, Mohamed M. ; Tholpady, Sunil S. ; Kessler, Jessica D. ; Morgan, Raymond F. ; Ogle, Roy C. / BMP-9-transduced prefabricated muscular flaps for the treatment of bony defects. In: Journal of Craniofacial Surgery. 2004 ; Vol. 15, No. 5.
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