Body Surface Area Prediction in Normal, Hypermuscular, and Obese Mice

Michael C. Cheung, Paul B. Spalding, Juan C. Gutierrez, Wayne Balkan, Nicholas Namias, Leonidas Koniaris, Teresa Zimmers

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Background: Accurate determination of body surface area (BSA) in experimental animals is essential for modeling effects of burn injury or drug metabolism. Two-dimensional surface area is related to three-dimensional body volume, which in turn can be estimated from body mass. The Meeh equation relates body surface area to the two-thirds power of body mass, through a constant, k, which must be determined empirically by species and size. We found older values of k overestimated BSA in certain mice; thus we determined empirically k for various strains of normal, obese, and hypermuscular mice. Materials and methods: BSA was computed from digitally scanned pelts and nonlinear regression analysis was used to determine the best-fit k. Results: The empirically determined k for C57BL/6J mice of 9.82 was not significantly different from other inbred and outbred mouse strains of normal body composition. However, mean k of the nearly spheroid, obese leprdb/db mice (k = 8.29) was significantly lower than for normals, as were values for dumbbell-shaped, hypermuscular mice with either targeted deletion of the myostatin gene (Mstn) (k = 8.48) or with skeletal muscle specific expression of a dominant negative myostatin receptor (Acvr2b) (k = 8.80). Conclusions: Hypermuscular and obese mice differ substantially from normals in shape and density, resulting in considerably altered k values. This suggests Meeh constants should be determined empirically for animals of altered body composition. Use of these new, improved Meeh constants will allow greater accuracy in experimental models of burn injury and pharmacokinetics.

Original languageEnglish (US)
Pages (from-to)326-331
Number of pages6
JournalJournal of Surgical Research
Volume153
Issue number2
DOIs
StatePublished - May 15 2009
Externally publishedYes

Fingerprint

Obese Mice
Body Surface Area
Myostatin
Body Composition
Inbred Strains Mice
Gene Deletion
Wounds and Injuries
Inbred C57BL Mouse
Skeletal Muscle
Theoretical Models
Pharmacokinetics
Regression Analysis
Pharmaceutical Preparations

Keywords

  • allometry
  • body composition
  • body weights and measure
  • burn
  • genetically modified mice
  • Meeh constant
  • pharmacokinetics
  • skin

ASJC Scopus subject areas

  • Surgery

Cite this

Body Surface Area Prediction in Normal, Hypermuscular, and Obese Mice. / Cheung, Michael C.; Spalding, Paul B.; Gutierrez, Juan C.; Balkan, Wayne; Namias, Nicholas; Koniaris, Leonidas; Zimmers, Teresa.

In: Journal of Surgical Research, Vol. 153, No. 2, 15.05.2009, p. 326-331.

Research output: Contribution to journalArticle

Cheung, Michael C. ; Spalding, Paul B. ; Gutierrez, Juan C. ; Balkan, Wayne ; Namias, Nicholas ; Koniaris, Leonidas ; Zimmers, Teresa. / Body Surface Area Prediction in Normal, Hypermuscular, and Obese Mice. In: Journal of Surgical Research. 2009 ; Vol. 153, No. 2. pp. 326-331.
@article{2909d507209b4130b63047a3195210e3,
title = "Body Surface Area Prediction in Normal, Hypermuscular, and Obese Mice",
abstract = "Background: Accurate determination of body surface area (BSA) in experimental animals is essential for modeling effects of burn injury or drug metabolism. Two-dimensional surface area is related to three-dimensional body volume, which in turn can be estimated from body mass. The Meeh equation relates body surface area to the two-thirds power of body mass, through a constant, k, which must be determined empirically by species and size. We found older values of k overestimated BSA in certain mice; thus we determined empirically k for various strains of normal, obese, and hypermuscular mice. Materials and methods: BSA was computed from digitally scanned pelts and nonlinear regression analysis was used to determine the best-fit k. Results: The empirically determined k for C57BL/6J mice of 9.82 was not significantly different from other inbred and outbred mouse strains of normal body composition. However, mean k of the nearly spheroid, obese leprdb/db mice (k = 8.29) was significantly lower than for normals, as were values for dumbbell-shaped, hypermuscular mice with either targeted deletion of the myostatin gene (Mstn) (k = 8.48) or with skeletal muscle specific expression of a dominant negative myostatin receptor (Acvr2b) (k = 8.80). Conclusions: Hypermuscular and obese mice differ substantially from normals in shape and density, resulting in considerably altered k values. This suggests Meeh constants should be determined empirically for animals of altered body composition. Use of these new, improved Meeh constants will allow greater accuracy in experimental models of burn injury and pharmacokinetics.",
keywords = "allometry, body composition, body weights and measure, burn, genetically modified mice, Meeh constant, pharmacokinetics, skin",
author = "Cheung, {Michael C.} and Spalding, {Paul B.} and Gutierrez, {Juan C.} and Wayne Balkan and Nicholas Namias and Leonidas Koniaris and Teresa Zimmers",
year = "2009",
month = "5",
day = "15",
doi = "10.1016/j.jss.2008.05.002",
language = "English (US)",
volume = "153",
pages = "326--331",
journal = "Journal of Surgical Research",
issn = "0022-4804",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Body Surface Area Prediction in Normal, Hypermuscular, and Obese Mice

AU - Cheung, Michael C.

AU - Spalding, Paul B.

AU - Gutierrez, Juan C.

AU - Balkan, Wayne

AU - Namias, Nicholas

AU - Koniaris, Leonidas

AU - Zimmers, Teresa

PY - 2009/5/15

Y1 - 2009/5/15

N2 - Background: Accurate determination of body surface area (BSA) in experimental animals is essential for modeling effects of burn injury or drug metabolism. Two-dimensional surface area is related to three-dimensional body volume, which in turn can be estimated from body mass. The Meeh equation relates body surface area to the two-thirds power of body mass, through a constant, k, which must be determined empirically by species and size. We found older values of k overestimated BSA in certain mice; thus we determined empirically k for various strains of normal, obese, and hypermuscular mice. Materials and methods: BSA was computed from digitally scanned pelts and nonlinear regression analysis was used to determine the best-fit k. Results: The empirically determined k for C57BL/6J mice of 9.82 was not significantly different from other inbred and outbred mouse strains of normal body composition. However, mean k of the nearly spheroid, obese leprdb/db mice (k = 8.29) was significantly lower than for normals, as were values for dumbbell-shaped, hypermuscular mice with either targeted deletion of the myostatin gene (Mstn) (k = 8.48) or with skeletal muscle specific expression of a dominant negative myostatin receptor (Acvr2b) (k = 8.80). Conclusions: Hypermuscular and obese mice differ substantially from normals in shape and density, resulting in considerably altered k values. This suggests Meeh constants should be determined empirically for animals of altered body composition. Use of these new, improved Meeh constants will allow greater accuracy in experimental models of burn injury and pharmacokinetics.

AB - Background: Accurate determination of body surface area (BSA) in experimental animals is essential for modeling effects of burn injury or drug metabolism. Two-dimensional surface area is related to three-dimensional body volume, which in turn can be estimated from body mass. The Meeh equation relates body surface area to the two-thirds power of body mass, through a constant, k, which must be determined empirically by species and size. We found older values of k overestimated BSA in certain mice; thus we determined empirically k for various strains of normal, obese, and hypermuscular mice. Materials and methods: BSA was computed from digitally scanned pelts and nonlinear regression analysis was used to determine the best-fit k. Results: The empirically determined k for C57BL/6J mice of 9.82 was not significantly different from other inbred and outbred mouse strains of normal body composition. However, mean k of the nearly spheroid, obese leprdb/db mice (k = 8.29) was significantly lower than for normals, as were values for dumbbell-shaped, hypermuscular mice with either targeted deletion of the myostatin gene (Mstn) (k = 8.48) or with skeletal muscle specific expression of a dominant negative myostatin receptor (Acvr2b) (k = 8.80). Conclusions: Hypermuscular and obese mice differ substantially from normals in shape and density, resulting in considerably altered k values. This suggests Meeh constants should be determined empirically for animals of altered body composition. Use of these new, improved Meeh constants will allow greater accuracy in experimental models of burn injury and pharmacokinetics.

KW - allometry

KW - body composition

KW - body weights and measure

KW - burn

KW - genetically modified mice

KW - Meeh constant

KW - pharmacokinetics

KW - skin

UR - http://www.scopus.com/inward/record.url?scp=64849109000&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=64849109000&partnerID=8YFLogxK

U2 - 10.1016/j.jss.2008.05.002

DO - 10.1016/j.jss.2008.05.002

M3 - Article

C2 - 18952236

AN - SCOPUS:64849109000

VL - 153

SP - 326

EP - 331

JO - Journal of Surgical Research

JF - Journal of Surgical Research

SN - 0022-4804

IS - 2

ER -