Bond-selective imaging of deep tissue through the optical window between 1600 and 1850 nm

Pu Wang, Han Wei Wang, Michael Sturek, Ji Xin Cheng

Research output: Contribution to journalArticlepeer-review

62 Scopus citations


We report the employment of an optical window between 1600 nm and 1850 nm for bond-selective deep tissue imaging through harmonic vibrational excitation and acoustic detection of resultant pressure waves. In this window where a local minimum of water absorption resides, we found a 5 times enhancement of photoacoustic signal by first overtone excitation of the methylene group CH2 at 1730 nm, compared to the second overtone excitation at 1210 nm. The enhancement allows 3D mapping of intramuscular fat with improved contrast and of lipid deposition inside an atherosclerotic artery wall in the presence of blood. Moreover, lipid and protein are differentiated based on the first overtone absorption profiles of CH2 and methyl group CH3 in this window.

Original languageEnglish (US)
Pages (from-to)25-32
Number of pages8
JournalJournal of Biophotonics
Issue number1
StatePublished - Jan 2012


  • Atherosclerosis
  • Deep tissue imaging
  • Optical window
  • Photoacoustic imaging

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)
  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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