Bone implants

Processing, embedding, cutting, and histopathology

Nancy W. Troiano, Melissa Kacena

Research output: Contribution to journalArticle

Abstract

The purpose of this review is to discuss the processing, embedding, and cutting/sectioning techniques used for bone implants and their associated histopathology. It is well known that all specimen-handling procedures, such as fixation, infiltration, embedding, and cutting/sectioning, impact the quality of the histological, histochemical, and immunohistochemical analysis of specimens. Bone specimens containing implants are particularly difficult specimens to handle for several reasons. First, specimens and their associated embedding material must be of equivalent hardness for quality sections to be obtained. For bone this means either decalcification and subsequent embedding in paraffin or embedding bone specimens in plastic. Second, the hardness of the implant material must be taken into consideration. In addition to influencing the type of embedding material used, the type of implant and whether the bone is decalcified also dictates which types of equipment must be used for cutting/sectioning specimens. For example, as described in more detail below, metal, ceramic, and glass implants must be cut and ground. This requires specialized equipment such as saw microtomes, diamond saws, and grinders/polishers. Third, implants themselves may be altered by the chemicals used in standard histological processing of tissue specimens. Finally, the overall processing of the specimen and implant can impact the interpretation of the results. These bone implant issues are the focus of this review.

Original languageEnglish (US)
Pages (from-to)253-264
Number of pages12
JournalJournal of Histotechnology
Volume29
Issue number4
StatePublished - Dec 2006
Externally publishedYes

Fingerprint

Bone and Bones
Hardness
Paraffin Embedding
Specimen Handling
Equipment and Supplies
Diamond
Plastics
Metals

Keywords

  • Acrylic
  • Bioactive glass
  • Biodegradable polymers
  • Ceramic
  • Fixation
  • Metal
  • Paraffin
  • Pathology
  • Plastic

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Bone implants : Processing, embedding, cutting, and histopathology. / Troiano, Nancy W.; Kacena, Melissa.

In: Journal of Histotechnology, Vol. 29, No. 4, 12.2006, p. 253-264.

Research output: Contribution to journalArticle

@article{6636c0df145b49c5acb53b471c1d8989,
title = "Bone implants: Processing, embedding, cutting, and histopathology",
abstract = "The purpose of this review is to discuss the processing, embedding, and cutting/sectioning techniques used for bone implants and their associated histopathology. It is well known that all specimen-handling procedures, such as fixation, infiltration, embedding, and cutting/sectioning, impact the quality of the histological, histochemical, and immunohistochemical analysis of specimens. Bone specimens containing implants are particularly difficult specimens to handle for several reasons. First, specimens and their associated embedding material must be of equivalent hardness for quality sections to be obtained. For bone this means either decalcification and subsequent embedding in paraffin or embedding bone specimens in plastic. Second, the hardness of the implant material must be taken into consideration. In addition to influencing the type of embedding material used, the type of implant and whether the bone is decalcified also dictates which types of equipment must be used for cutting/sectioning specimens. For example, as described in more detail below, metal, ceramic, and glass implants must be cut and ground. This requires specialized equipment such as saw microtomes, diamond saws, and grinders/polishers. Third, implants themselves may be altered by the chemicals used in standard histological processing of tissue specimens. Finally, the overall processing of the specimen and implant can impact the interpretation of the results. These bone implant issues are the focus of this review.",
keywords = "Acrylic, Bioactive glass, Biodegradable polymers, Ceramic, Fixation, Metal, Paraffin, Pathology, Plastic",
author = "Troiano, {Nancy W.} and Melissa Kacena",
year = "2006",
month = "12",
language = "English (US)",
volume = "29",
pages = "253--264",
journal = "Journal of Histotechnology",
issn = "0147-8885",
publisher = "Maney Publishing",
number = "4",

}

TY - JOUR

T1 - Bone implants

T2 - Processing, embedding, cutting, and histopathology

AU - Troiano, Nancy W.

AU - Kacena, Melissa

PY - 2006/12

Y1 - 2006/12

N2 - The purpose of this review is to discuss the processing, embedding, and cutting/sectioning techniques used for bone implants and their associated histopathology. It is well known that all specimen-handling procedures, such as fixation, infiltration, embedding, and cutting/sectioning, impact the quality of the histological, histochemical, and immunohistochemical analysis of specimens. Bone specimens containing implants are particularly difficult specimens to handle for several reasons. First, specimens and their associated embedding material must be of equivalent hardness for quality sections to be obtained. For bone this means either decalcification and subsequent embedding in paraffin or embedding bone specimens in plastic. Second, the hardness of the implant material must be taken into consideration. In addition to influencing the type of embedding material used, the type of implant and whether the bone is decalcified also dictates which types of equipment must be used for cutting/sectioning specimens. For example, as described in more detail below, metal, ceramic, and glass implants must be cut and ground. This requires specialized equipment such as saw microtomes, diamond saws, and grinders/polishers. Third, implants themselves may be altered by the chemicals used in standard histological processing of tissue specimens. Finally, the overall processing of the specimen and implant can impact the interpretation of the results. These bone implant issues are the focus of this review.

AB - The purpose of this review is to discuss the processing, embedding, and cutting/sectioning techniques used for bone implants and their associated histopathology. It is well known that all specimen-handling procedures, such as fixation, infiltration, embedding, and cutting/sectioning, impact the quality of the histological, histochemical, and immunohistochemical analysis of specimens. Bone specimens containing implants are particularly difficult specimens to handle for several reasons. First, specimens and their associated embedding material must be of equivalent hardness for quality sections to be obtained. For bone this means either decalcification and subsequent embedding in paraffin or embedding bone specimens in plastic. Second, the hardness of the implant material must be taken into consideration. In addition to influencing the type of embedding material used, the type of implant and whether the bone is decalcified also dictates which types of equipment must be used for cutting/sectioning specimens. For example, as described in more detail below, metal, ceramic, and glass implants must be cut and ground. This requires specialized equipment such as saw microtomes, diamond saws, and grinders/polishers. Third, implants themselves may be altered by the chemicals used in standard histological processing of tissue specimens. Finally, the overall processing of the specimen and implant can impact the interpretation of the results. These bone implant issues are the focus of this review.

KW - Acrylic

KW - Bioactive glass

KW - Biodegradable polymers

KW - Ceramic

KW - Fixation

KW - Metal

KW - Paraffin

KW - Pathology

KW - Plastic

UR - http://www.scopus.com/inward/record.url?scp=34548695413&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34548695413&partnerID=8YFLogxK

M3 - Article

VL - 29

SP - 253

EP - 264

JO - Journal of Histotechnology

JF - Journal of Histotechnology

SN - 0147-8885

IS - 4

ER -